Utp8p Is an Essential Intranuclear Component of the Nuclear tRNA Export Machinery of Saccharomyces cerevisiae

Steiner-Mosonyi, Marta; Leslie, Deena M.; Dehghani, Hesam; Aitchison, John D.; Mangroo, Dev

doi:10.1074/jbc.m302779200

Cited by 36 publications

(77 citation statements)

References 60 publications

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“…Examples of cross talk between ribosome assembly and other cellular processes mediated through shared components include replication, rRNA transcription, tRNA export, cell cycle control, and stress response (e.g., Angermayr and Bandlow 2002;Du and Stillman 2002;Steiner-Mosonyi et al 2003;Killian et al 2004;Bernstein et al 2007;Rudra et al 2007;Strub et al 2007;Jwa et al 2008), and this cross talk is more evolved in higher organisms. Because the connection to the cell cycle has been recently and expertly reviewed (Dez and Tollervey 2004;, it will not be covered here.…”

Section: Cross Talk Of Ribosome Biogenesis Through Shared Componentsmentioning

confidence: 99%

Powering through ribosome assembly

Strunk¹,

Karbstein²

2009

RNA

193

168

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Ribosome assembly is required for cell growth in all organisms. Classic in vitro work in bacteria has led to a detailed understanding of the biophysical, thermodynamic, and structural basis for the ordered and correct assembly of ribosomal proteins on ribosomal RNA. Furthermore, it has enabled reconstitution of active subunits from ribosomal RNA and proteins in vitro. Nevertheless, recent work has shown that eukaryotic ribosome assembly requires a large macromolecular machinery in vivo. Many of these assembly factors such as ATPases, GTPases, and kinases hydrolyze nucleotide triphosphates. Because these enzymes are likely regulatory proteins, much work to date has focused on understanding their role in the assembly process. Here, we review these factors, as well as other sources of energy, and their roles in the ribosome assembly process. In addition, we propose roles of energy-releasing enzymes in the assembly process, to explain why energy is used for a process that occurs largely spontaneously in bacteria. Finally, we use literature data to suggest testable models for how these enzymes could be used as targets for regulation of ribosome assembly.

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Section: Cross Talk Of Ribosome Biogenesis Through Shared Componentsmentioning

confidence: 99%

Powering through ribosome assembly

Strunk¹,

Karbstein²

2009

RNA

193

168

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“…The former RNAs were kept in TE [10 mM Tris-HCl, pH 7.5, 1 mM EDTA] and run on a 7.0 M urea/10% w/v polyacrylamide gel in TBE, while the latter RNAs were dissolved in AE [10 mM sodium acetate, pH 4.5, 1.0 mM EDTA] and run on a 10 M urea/10% w/v polyacrylamide gel in 0.10 M NaOAc, pH 5.0. Deacylation of tRNAs was performed in 50 mM Tris-HCl, pH 9.0 at 37°C for 1 h (Steiner-Mosonyi et al 2003). After transfer to charged nylon membranes (Hybond N + , GE Healthcare Biosciences), a particular RNA was decorated with an appropriate digoxigeninlabeled oligonucleotide probe (Roche Diagnostics), and the signal was developed by ECF (GE Healthcare Biosciences) and read with a Storm 860 Image Analyzer (GE Healthcare Biosciences).…”

Section: Rna Analysismentioning

confidence: 99%

The intron of tRNA-Trp_CCA is dispensable for growth and translation of Saccharomyces cerevisiae

Mori¹,

Kajita²,

Endo³

et al. 2011

RNA

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A part of eukaryotic tRNA genes harbor an intron at one nucleotide 39 to the anticodon, so that removal of the intron is an essential processing step for tRNA maturation. While some tRNA introns have important roles in modification of certain nucleotides, essentiality of the tRNA intron in eukaryotes has not been tested extensively. This is partly because most of the eukaryotic genomes have multiple genes encoding an isoacceptor tRNA. Here, we examined whether the intron of tRNA-Trp CCA genes, six copies of which are scattered on the genome of yeast, Saccharomyces cerevisiae, is essential for growth or translation of the yeast in vivo. We devised a procedure to remove all of the tRNA introns from the yeast genome iteratively with marker cassettes containing both positive and negative markers. Using this procedure, we removed all the introns from the six tRNATrp CCA genes, and found that the intronless strain grew normally and expressed tRNA-Trp CCA in an amount similar to that of the wild-type genes. Neither incorporation of 35 S-labeled amino acids into a TCA-insoluble fraction nor the major protein pattern on SDS-PAGE/2D gel were affected by complete removal of the intron, while expression levels of some proteins were marginally affected. Therefore, the tRNA-Trp CCA intron is dispensable for growth and bulk translation of the yeast. This raises the possibility that some mechanism other than selective pressure from translational efficiency maintains the tRNA intron on the yeast genome.

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“…), tRNA-specific exportin (Los1p), CCA transferase also acting on tRNA export (Cca1p), aminoacyl tRNA synthetases (ARS, for example Tys1p, etc. ), nucleolar factor Utp8p and so on [15][16][17][18][19][20][21]. All the facts mentioned above led us to the unexpected notion that tRNAs must be re-imported from the cytoplasm into the nucleus after maturation.…”

Section: Introductionmentioning

confidence: 97%

tRNA, new aspects in intracellular dynamics

Yoshihisa

2006

Cell. Mol. Life Sci.

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The nuclear envelope divides a eukaryotic cell into two compartments, the nucleus and the cytoplasm. Transcription and maturation of RNAs encoded on nuclear chromosomes are carried out in the nucleus, while the proteins coded by these RNAs are translated and processed in the cytoplasm. Cytosolic tRNAs, essential factors for translation, are transcribed in the nucleus and undergo extensive processing before reaching functionality. It had previously been believed that tRNAs have only one-way tickets to pass through the nuclear envelope after maturation in the nucleus, and that the small amounts of mature tRNAs found in the nucleus are biosynthetic intermediates. However, two reports from our lab and Anita Hopper's group recently demonstrated that tRNAs have multi-round commuter tickets to shuttle between the nucleus and the cytoplasm in the yeast Saccharomyces cerevisiae. In fact, various tRNA species, including aminoacylated full-length tRNAs and 3' end-shortened tRNAs, are actively imported into the nucleus under various conditions. These findings force a reconsideration of our view of intracellular dynamics of tRNAs, and re-evaluations of the physiological meanings of the nuclear mature tRNAs.

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Utp8p Is an Essential Intranuclear Component of the Nuclear tRNA Export Machinery of Saccharomyces cerevisiae

Cited by 36 publications

References 60 publications

Powering through ribosome assembly

Powering through ribosome assembly

The intron of tRNA-Trp_CCA is dispensable for growth and translation of Saccharomyces cerevisiae

tRNA, new aspects in intracellular dynamics

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Utp8p Is an Essential Intranuclear Component of the Nuclear tRNA Export Machinery of Saccharomyces cerevisiae

Cited by 36 publications

References 60 publications

Powering through ribosome assembly

Powering through ribosome assembly

The intron of tRNA-TrpCCA is dispensable for growth and translation of Saccharomyces cerevisiae

tRNA, new aspects in intracellular dynamics

Contact Info

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Resources

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The intron of tRNA-Trp_CCA is dispensable for growth and translation of Saccharomyces cerevisiae