UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

Dalmais, Marion; Schmidt, Julien; Signor, Christine Le; Moussy, F.; Burstin, Judith; Savois, Vincent; Aubert, Grégoire; Brunaud, Véronique; Oliveira, Yannick de; Guichard, Cécile; Thompson, Richard; Bendahmane, Abdelhafid

doi:10.1186/gb-2008-9-2-r43

Cited by 154 publications

(141 citation statements)

References 45 publications

(40 reference statements)

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“…The psfulc-2 mutant was generated from line NGB5839 by EMS mutagenesis 27 . The psfulc-3 mutant was isolated by reverse screening in an EMS-mutagenized TILLING population of cultivar Caméor 28 . The veg2-1 mutant was isolated from fast-neutron mutagenesis of cv.…”

Section: Methodsmentioning

confidence: 99%

“…4b). A third PsFULc mutant allele (psfulc-3), carrying a Q102STOP mutation, was identified in reverse genetic screening of an EMS-mutagenized Targeting-Induced Local Lesions in Genomes (TILLING) population 28 . Like psfulc-2, this mutant also showed a clear veg1 phenotype (Fig.…”

Section: Veg1 Is Required To Make Secondary Inflorescencesmentioning

confidence: 99%

“…DNA isolation and pooling, PCR amplification and mutation detection were performed, as previously described 28 . For primer sequences in this and following sections in Methods see Supplementary Table S1.…”

Section: Tillingmentioning

confidence: 99%

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VEGETATIVE1 is essential for development of the compound inflorescence in pea

et al. 2012

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unravelling the basis of variation in inflorescence architecture is important to understanding how the huge diversity in plant form has been generated. Inflorescences are divided between simple, as in Arabidopsis, with flowers directly formed at the main primary inflorescence axis, and compound, as in legumes, where they are formed at secondary or even higher order axes. The formation of secondary inflorescences predicts a novel genetic function in the development of the compound inflorescences. Here we show that in pea this function is controlled by VEGETATIVE1 (VEG1), whose mutation replaces secondary inflorescences by vegetative branches. We identify VEG1 as an AGL79-like mADs-box gene that specifies secondary inflorescence meristem identity. VEG1 misexpression in meristem identity mutants causes ectopic secondary inflorescence formation, suggesting a model for compound inflorescence development based on antagonistic interactions between VEG1 and genes conferring primary inflorescence and floral identity. our study defines a novel mechanism to generate inflorescence complexity.

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Section: Methodsmentioning

confidence: 99%

Section: Veg1 Is Required To Make Secondary Inflorescencesmentioning

confidence: 99%

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VEGETATIVE1 is essential for development of the compound inflorescence in pea

et al. 2012

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“…In pea, a transcription atlas (RNAseq) and gene-based maps (Bordat et al, 2011) will aid translation of genomic knowledge to practical breeding. Moreover, the pea variety Cameor was used to develop the TILLING mutant population (Dalmais et al, 2008) and to construct a BAC library, both essential tools for positional cloning and pea genome sequencing (reviewed in Smýkal et al, 2012;Smýkal and Konečná, 2014). Increased knowledge of the pea genome has not only a scientific but also a great educational and social impact, owing to seminal work of J. G. Mendel (1865-66) (Schwarzbach et al, 2014).…”

Section: Impact Of Genomics For Crop Legumementioning

confidence: 99%

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

Smýkal

Coyne

Ambrose

et al. 2014

Critical Reviews in Plant Sciences

269

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“…PsTAR2 mutants were identified from an ethyl methanesulfonate mutant collection of 4,800 pea lines using TILLING screening as described by Dalmais et al (2008). Details of all primer sequences are given in Supplemental Table S2.…”

Section: Plant Materialsmentioning

confidence: 99%

Biosynthesis of the Halogenated Auxin, 4-Chloroindole-3-Acetic Acid

et al. 2012

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Seeds of several agriculturally important legumes are rich sources of the only halogenated plant hormone, 4-chloroindole-3-acetic acid. However, the biosynthesis of this auxin is poorly understood. Here, we show that in pea (Pisum sativum) seeds, 4-chloroindole-3-acetic acid is synthesized via the novel intermediate 4-chloroindole-3-pyruvic acid, which is produced from 4-chlorotryptophan by two aminotransferases, TRYPTOPHAN AMINOTRANSFERASE RELATED1 and TRYPTOPHAN AMINOTRANSFERASE RELATED2. We characterize a tar2 mutant, obtained by Targeting Induced Local Lesions in Genomes, the seeds of which contain dramatically reduced 4-chloroindole-3-acetic acid levels as they mature. We also show that the widespread auxin, indole-3-acetic acid, is synthesized by a parallel pathway in pea.

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UTILLdb, a Pisum sativum in silico forward and reverse genetics tool

Abstract: UTILLdb is a database of phenotypic and sequence information on mutant genes from a reference Pisum sativum EMS-mutant population.

Cited by 154 publications

References 45 publications

VEGETATIVE1 is essential for development of the compound inflorescence in pea

VEGETATIVE1 is essential for development of the compound inflorescence in pea

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

Biosynthesis of the Halogenated Auxin, 4-Chloroindole-3-Acetic Acid

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