Utilizing Wild Species for Marker-Assisted Selection of Crop Timing and Quality Traits in Petunia

Tychonievich, Joseph; Wangchu, L.; Barry, Cornelius S.; Warner, Ryan M.

doi:10.17660/actahortic.2013.1000.65

Cited by 4 publications

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“…For example, 24 SSRs were polymorphic between P. integrifolia and P. axillaris , 28 were polymorphic between P. axillaris and P. exserta , and 24 were polymorphic between P. integrifolia and P. exserta. This success rate was higher than for our previous results developing SSR markers from P. axillaris ESTs [ 66 ].…”

Section: Resultsmentioning

confidence: 64%

Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.

et al. 2015

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BackgroundPetunia (Petunia × hybrida), derived from a hybrid between P. axillaris and P. integrifolia, is one of the most economically important bedding plant crops and Petunia spp. serve as model systems for investigating the mechanisms underlying diverse mating systems and pollination syndromes. In addition, we have previously described genetic variation and quantitative trait loci (QTL) related to petunia development rate and morphology, which represent important breeding targets for the floriculture industry to improve crop production and performance. Despite the importance of petunia as a crop, the floriculture industry has been slow to adopt marker assisted selection to facilitate breeding strategies and there remains a limited availability of sequences and molecular markers from the genus compared to other economically important members of the Solanaceae family such as tomato, potato and pepper.ResultsHere we report the de novo assembly, annotation and characterization of transcriptomes from P. axillaris, P. exserta and P. integrifolia. Each transcriptome assembly was derived from five tissue libraries (callus, 3-week old seedlings, shoot apices, flowers of mixed developmental stages, and trichomes). A total of 74,573, 54,913, and 104,739 assembled transcripts were recovered from P. axillaris, P. exserta and P. integrifolia, respectively and following removal of multiple isoforms, 32,994 P. axillaris, 30,225 P. exserta, and 33,540 P. integrifolia high quality representative transcripts were extracted for annotation and expression analysis. The transcriptome data was mined for single nucleotide polymorphisms (SNP) and simple sequence repeat (SSR) markers, yielding 89,007 high quality SNPs and 2949 SSRs, respectively. 15,701 SNPs were computationally converted into user-friendly cleaved amplified polymorphic sequence (CAPS) markers and a subset of SNP and CAPS markers were experimentally verified. CAPS markers developed from plastochron-related homologous transcripts from P. axillaris were mapped in an interspecific Petunia population and evaluated for co-localization with QTL for development rate.ConclusionsThe high quality of the three Petunia spp. transcriptomes coupled with the utility of the SNP data will serve as a resource for further exploration of genetic diversity within the genus and will facilitate efforts to develop genetic and physical maps to aid the identification of QTL associated with traits of interest.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1931-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 64%

Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.

et al. 2015

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“…Despite the advantages of molecular markers, within ornamental breeding they are only used routinely for cultivar development in economically important crops such as rose, carnation, chrysanthemum, petunia, and lily (Onozaki et al, 2004;Von Malek et al, 2000;Su et al, 2019;Tychonievich et al, 2011;Van Tuyl et al, 2018). High levels of heterozygosity, different genetic resources controlling the traits, involvement of inter-and intraspecific crosses and genome complexities across the world all limit the usefulness of molecular markers across wider germplasms in ornamental species.…”

Section: Marker Assisted Breeding and Genomics Technologymentioning

confidence: 99%

Ornamental plant breeding: entering a new era?

Huylenbroeck

Bhattarai

2022

Ornam. Hortic.

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During the last century, plant breeding of crops and ornamentals has become an important business. Mendel’s principles of inheritance of traits laid the foundation of today’s modern genetics. Although intra- and interspecific cross breeding, chromosome doubling and mutation breeding still form the cornerstone of ornamental plant breeding, the speed of plant breeding has significantly increased since Mendel’s time. New genomic tools now offer possibilities for advanced precision breeding and selection towards more difficult to assess or quantitative traits. Today’s ornamental plant breeding is a complicated exercise, characterized by new and rapidly-changing challenges. Partnerships between industry and research will be essential to implement available technologies in minor crops.

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Molecular Breeding in Ornamental Crops: Current Trends and Future Prospects in the Genomic Era

Husain,

Pandey,

Singh

et al. 2024

Ornamental Horticulture: Latest Cultivation Practices and Breeding Technologies

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Utilizing Wild Species for Marker-Assisted Selection of Crop Timing and Quality Traits in Petunia

Cited by 4 publications

References 0 publications

Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.

Transcriptome-enabled marker discovery and mapping of plastochron-related genes in Petunia spp.

Ornamental plant breeding: entering a new era?

Molecular Breeding in Ornamental Crops: Current Trends and Future Prospects in the Genomic Era

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