Utilizing pyrene-degrading endophytic bacteria to reduce the risk of plant pyrene contamination

Sun, Kai; Liu, Juan; Li, Jin; Gao, Yanzheng

doi:10.1007/s11104-013-1875-x

Cited by 61 publications

(33 citation statements)

References 46 publications

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“…Plants provide residency and nutrients for bacteria. In exchange, endophytic bacteria can improve plant growth by fixing nitrogen, increasing nutrient uptake, improving plant tolerance of PAH, activating plant enzyme, hormone, siderophores, and other organics (Saïdi et al 2013;Sun et al 2013;Teng et al 2011). Plantsendophyte associations rely on very close beneficial interactions in PAH phytoremediation.…”

Section: Discussionmentioning

confidence: 99%

Study of phenanthrene utilizing bacterial consortia associated with cowpea (Vigna unguiculata) root nodules

Sun

Crowley

Wei

2015

World J Microbiol Biotechnol

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Many legumes have been selected as model plants to degrade organic contaminants with their special associated rhizosphere microbes in soil. However, the function of root nodules during microbe-assisted phytoremediation is not clear. A pot study was conducted to examine phenanthrene (PHE) utilizing bacteria associated with root nodules and the effects of cowpea root nodules on phytoremediation in two different types of soils (freshly contaminated soil and aged contaminated soil). Cowpea nodules in freshly-contaminated soil showed less damage in comparison to the aged-contaminated soil, both morphologically and ultra-structurally by scanning electron microscopy. The study of polycyclic aromatic hydrocarbon (PAH) attenuation conducted by high performance liquid chromatography revealed that more PAH was eliminated from liquid culture around nodulated roots than nodule-free roots. PAH sublimation and denaturation gradient gel electrophoresis were applied to analyze the capability and diversity of PAH degrading bacteria from the following four parts of rhizo-microzone: bulk soil, root surface, nodule surface and nodule inside. The results indicated that the surface and inside of cowpea root nodules were colonized with bacterial consortia that utilized PHE. Our results demonstrated that root nodules not only fixed nitrogen, but also enriched PAH-utilizing microorganisms both inside and outside of the nodules. Legume nodules may have biotechnological values for PAH degradation.

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Section: Discussionmentioning

confidence: 99%

Study of phenanthrene utilizing bacterial consortia associated with cowpea (Vigna unguiculata) root nodules

Sun

Crowley

Wei

2015

World J Microbiol Biotechnol

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“…The quantification was done with a five point's calibration plot (1,5,10,20, 50 mgL-1) of the aliphatic hydrocarbon ranges from C8 to C40. Quantitation for each carbon number was done by summarizing the area for each carbon number at the respective retention time and correlated with the area for individual carbon number of the calibration standards.…”

Section: Extraction Of the Soil For Tph Analysismentioning

confidence: 99%

“…However, plants have shown the ability to take up most of these PAHs and their toxic metabolites. This forms the major entry route of the compound into the food chain and the effects is been felt in almost every part of the ecosystem [5].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Plants at Petroleum Contaminated Site for Phytoremediation

Anyasi¹,

Atagana²

2017

International Conference of Recent Trends in Environmental Science and Engineering

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-The study is aimed at identifying native plants growing around petroleum hydrocarbon contaminated site in Umuahia, Nigeria that has the ability to phytoremediate the contaminant from the soil. A total of 28 native plants in the area were sampled. Most of the plants demonstrated the ability to grow in a high concentration of the total petroleum hydrocarbon which is an indication that they can be used for phytoremediation of a petroleum hydrocarbon contaminated soil. Chromolaena odorata, Aspilla africana, Chloris babata, Pasparlum vaginatum, Bryophylum pinnatum, Paspalum scrobiculatum, Spondias mombim, Euragrostis atrovilens, Cyperus rotundus, Uvaria chamae all demonstrated phytoremediation characteristics in the contaminated site. Among those characteristics was the ability to survive the toxic nature of the contamination and to uptake part of the contamination. Pearson correlation demonstrated a significant and positive relations (r = 0.64, p = 0.003) (p ˂ 0.05) between the soil and root TPH. Using bioaccumulation factor as a module for phytoremediation resulted in Linear regression between TPH concentration in the soil and root tissue of Y = 1.8696x + 608.55. From this study, chromolaena odorata, Aspilla Africana and Uvaria chamae were selected as candidate plants for phytoremediation of PAHs.

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“…Endophytic bacteria can enhance plant growth, reduce plant disease, and stimulate plant resistance to harsh external environments (Ryan et al 2008;Glick 2014). Notably, certain endophytic bacteria can prevent/reduce the accumulation of organic pollutants in plants (Germaine et al 2009;Sun et al 2014b). For example, the inoculation of pea plants (Pisum sativum L.) with a Pseudomonas putida strain prevented 2,4-dichlorophenoxyacetic acid (2,4-D) accumulation (Germaine et al 2006).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, when ryegrass (Lolium multiflorum Lam) were colonized with endophytic Staphylococcus sp. strain BJ06 (isolated from Alopecurus aequalis Sobol), the concentrations of pyrene in ryegrass roots and shoots decreased, and were 31 and 44 % lower than those for the endophyte-free (non-inoculated) treatment (Sun et al 2014b). Determining an optimal plant colonization method is important for reducing the risk of plant organic contamination.…”

Section: Introductionmentioning

confidence: 99%

Inoculating plants with the endophytic bacterium Pseudomonas sp. Ph6-gfp to reduce phenanthrene contamination

Sun

Liu

Gao

et al. 2015

Environ Sci Pollut Res

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Plant organic contamination poses a serious threat to the safety of agricultural products and human health worldwide, and the association of endophytic bacteria with host plants may decrease organic pollutants in planta. In this study, we firstly determined the growth response and biofilm formation of endophytic Pseudomonas sp. Ph6-gfp, and then systematically evaluated the performance of different plant colonization methods (seed soaking (SS), root soaking (RS), leaf painting (LP)) for circumventing the risk of plant phenanthrene (PHE) contamination. After inoculation for 48 h, strain Ph6-gfp grew efficiently with PHE, oxalic acid, or malic acid as the sole sources of carbon and energy. Moreover, strain Ph6-gfp could form robust biofilms in LB medium. In greenhouse hydroponic experiments, strain Ph6-gfp could actively colonize inoculated plants internally, and plants colonized with Ph6-gfp showed a higher capacity for PHE removal. Compared with the Ph6-gfp-free treatment, the accumulations of PHE in Ph6-gfp-colonized plants via SS, RS, and LP were 20.1, 33.1, and 7.1 %, respectively, lower. Our results indicate that inoculating plants with Ph6-gfp could lower the risk of plant PHE contamination. RS was most efficient for improving PHE removal in whole plant bodies by increasing the cell numbers of Ph6-gfp in plant roots. The findings in this study provide an optimized method to strain Ph6-gfp reduce plant PAH residues, which may be applied to agricultural production in PAH-contaminated soil.

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Utilizing pyrene-degrading endophytic bacteria to reduce the risk of plant pyrene contamination

Cited by 61 publications

References 46 publications

Study of phenanthrene utilizing bacterial consortia associated with cowpea (Vigna unguiculata) root nodules

Study of phenanthrene utilizing bacterial consortia associated with cowpea (Vigna unguiculata) root nodules

Assessment of Plants at Petroleum Contaminated Site for Phytoremediation

Inoculating plants with the endophytic bacterium Pseudomonas sp. Ph6-gfp to reduce phenanthrene contamination

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