Utilizing Proteomic Approach to Analyze Potential Antioxidant Proteins in Plant against Irradiation

Abstract

Gamma-ray irradiation is an effective and clean method of sterilization by inactivating microorganisms. It can also be applied to induce anti-oxidants for future application. In this study, the mung bean (Vigna radiata) was exposed to gamma-ray irradiation under the dose of 0, 5 or 10 kGy. With increasing irradiation doses, the concentrations of malondiadehyde decreased while the levels of total flavonoids and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity increased. It has been shown that consuming flavonoids can provide protective effects. In addition, proteomic analysis identified several proteins having anti-oxidant activities in the 5 kGy irradiated group. These proteins are Apocytochrome f, Systemin receptor SR 160, DELLA protein DWARF8, DEAD-box ATP-dependent RNA helicase 9, ζ-carotene desaturase (ZDS), and Floral homeotic protein AGAMOUS. Our findings indicate that plants contain a variety of phytochemicals and antioxidant proteins which may effectively prevent oxidative stress caused by irradiated peroxidation.

Comparative Seed Proteome Profile Reveals No Alternation of Major Allergens in High-Yielding Mung Bean Cultivars

“…Mass spectrometry-based proteomics combined with advanced bioinformatics is a powerful platform not only to determine the seed proteome profile from many legumes − but also to identify, quantify, and demonstrate the conformational changes of many allergenic proteins. Despite being a crop high in nutrients, research has shown that mung beans are also cross-reactive with IgE. ,, Previous findings verified both naso-bronchial sensitization and the allergic reaction to mung bean consumption in clinical samples. ,, To date, only a handful of research papers have been published on the mung bean proteome. ,− However, to the best of our knowledge, no comparative analysis of the mung bean cultivar-specific seed proteome has been performed. The protein composition of mung bean seeds and sprouts was revealed in a recent study, which has enhanced our knowledge of the nutritional state of these plant parts and provided an in-depth understanding of the mechanisms involved in germination.…”