Utilizing primary HLA-G+ extravillous trophoblasts and HLA-G+ EVT-like cell lines to study maternal-fetal interactions

Purification of primary human placental leukocytes to study maternal-fetal interactions

“… Isolate primary HLA-G+ EVT from the same placenta or use established HLA-G+ EVT-like cell lines according to the protocols described by Hamilton et al. 2023 7 . Seed 5 × 10 4 HLA-G+ EVT in 100 μL Cell Culture Medium B on a collagen or fibronectin coated flat bottom 48 well culture plate.…”

“…Isolate primary HLA-G+ EVT from the same placenta or use established HLA-G+ EVT-like cell lines according to the protocols described by Hamilton et al. 2023 7 .…”