Utilizing HPLC to Analyze the Presence of Anticancerous Compounds Residing from the Isolate FM1005 (Xylaria sp.) Derived from Sinularia densa

Abstract

Marine organism-based natural products derived from endophytic fungi (microorganisms that reside in internal tissues of organisms and produce secondary metabolites) have been proposed as ground-breaking due to their potential to supply novel compounds for drug discovery. This investigation into possible marine-based medical applications derived from isolated compounds entailed a process inclusive of initial antiproliferative assays, the cultivation of targeted strains, and the implementation of compound separation methods for sub-fractional study and UV absorption analysis. Fractional compound analysis was conducted using a High-Performance Liquid Chromatography (HPLC) UV detector to derive probable identifications of compounds that presented antiproliferative activity. Various strains decreased the growth of A2780S (ovarian cancer), and DU-145 (prostate cancer) cell lines to the 20% and 50% viability range, respectively, and showed a degree of activity against MCF-10A (breast cancer) cell lines. Forty-eight compounds fell into the UV absorption rate range of 195-384nm, with Benzene, Rac-BINAP, and L-Histidine being commonly identified. These compound identifications can be utilized for structural analysis, novel compound discovery, and the creation of an anticancer drug that reduces the probability of cancer resurfacing in the patient. Further investigations focusing on pharmacokinetics, the biological mechanisms behind genetically inherited cancers, and the effects of specific compounds against them, such as the potential to induce apoptosis or activate inhibitory mechanisms, need to be undertaken. This research will continue to acknowledge a crucial balance point of environmental utilization, and awareness for potential future uses in the medical field.