Utilization of vegetable oil in the production of clavulanic acid by Streptomyces clavuligerusATCC 27064

Maranesi, G. L.; Neto, Álvaro de Baptista; Hokka, Carlos O.; Badino, Alberto Colli

doi:10.1007/s11274-004-2393-z

Cited by 33 publications

(27 citation statements)

References 24 publications

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“…The pH of the medium was adjusted to 6.8 with NaOH 5.0 M before autoclaving at 121°C for 15 min. The inoculum medium used in the cultivations, based on that proposed by Maranesi et al [20], had the following composition (in g/L distilled water): glycerol, 10; soybean flour (SF), 20; K 2 HPO 4 , 1.2; MnCl 2 ·4H 2 O, 0.001; FeSO 4 ·7H 2 O, 0.001; and ZnSO 4 ·7H 2 O, 0.001, pH 6.8. The composition of the production medium was similar to that used for the inoculum, except for the concentrations of SF and glycerol that were varied according to the selected experimental design.…”

Section: Culture Mediamentioning

confidence: 99%

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Viana

Carneiro-Cunha

Araújo

et al. 2009

Appl Biochem Biotechnol

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Clavulanic acid (CA) is a beta-lactam antibiotic, which has a potent beta-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28 degrees C, 30 degrees C, and 32 degrees C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32 degrees C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 g(X)/g(S) and 64.3 mg(P)/g(S), respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%.

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Section: Culture Mediamentioning

confidence: 99%

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Viana

Carneiro-Cunha

Araújo

et al. 2009

Appl Biochem Biotechnol

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“…There is a growing body of literature related to the comparison between airlift and conventional bioreactors for different applications [12][13][14][15][16][17]. Regarding clavulanic acid production, various studies can be found in the literature related to the improvements in the composition of culture media [6,8,[18][19][20], bioreactor operation mode [3,5,7,[21][22][23] and the influence of oxygen supply and agitation condition on CA production by S. clavuligerus in conventional bench scale bioreactor [4]. However, to the best of our knowledge no articles which compare the performances of conventional and non-conventional pneumatic bioreactors on CA production can be found.…”

Section: Introductionmentioning

confidence: 99%

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Cerri

Badino

2012

Bioprocess Biosyst Eng

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In biochemical processes involving filamentous microorganisms, the high shear rate may damage suspended cells leading to viability loss and cell disruption. In this work, the influence of the shear conditions in clavulanic acid (CA) production by Streptomyces clavuligerus was evaluated in a 4-dm(3) conventional stirred tank (STB) and in 6-dm(3) concentric-tube airlift (ALB) bioreactors. Batch cultivations were performed in a STB at 600 and 800 rpm and 0.5 vvm (cultivations B1 and B2) and in ALB at 3.0 and 4.1 vvm (cultivations A1 and A2) to define two initial oxygen transfer conditions in both bioreactors. The average shear rate ([Formula: see text]) of the cultivations was estimated using correlations of recent literature based on experimental data of rheological properties of the broth (consistency index, K, and flow index, n) and operating conditions, impeller speed (N) for STB and superficial gas velocity in the riser (UGR) for ALB. In the same oxygen transfer condition, the [Formula: see text] values for ALB were higher than those obtained in STB. The maximum [Formula: see text] presented a strong correlation with a maximum consistency index (K (max)) of the broth. Close values of maximum CA production were obtained in cultivations A1 and A2 (454 and 442 mg L(-1)) with similar maximum [Formula: see text] values of 4,247 and 4,225 s(-1). In cultivations B1 and B2, the maximum CA production of 269 and 402 mg L(-1) were reached with a maximum [Formula: see text] of 904 and 1,786 s(-1). The results show that high values of average shear rate increase the CA production regardless of the oxygen transfer condition and bioreactor model.

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“…This was then experimentally demonstrated by Ortiz et al, 4) where increased CA titers were observed when the initial concentration of oils was decreased. Maranesi et al 13) also studied the effect of using vegetable oils on the production of CA in S. clavuligerus ATCC 27064. They applied 16, 23, and 30 g/l of soybean oil, and obtained the highest productivity in the medium containing 23 g/l oil.…”

Section: Discussionmentioning

confidence: 99%

“…7H 2 O), and 1 ml/l of antifoam. 13,20) The seed medium was adjusted to pH 7.0 and sterilized at 121 C for 20 min, and the production medium was sterilized at 121 C for 70 min. A spore suspension (0.5 ml), which had been harvested from an agar plate, was added to a 100-ml baffled Erlenmeyer flask containing 20 ml of seed medium.…”

Section: Methodsmentioning

confidence: 99%

“…2) Although there have been many studies suggesting that oil is a preferred carbon source for increasing CA titers, few studies have systematically examined the kinds of fatty acids that are important to CA production and the cell growth of the producer microorganisms. 9,12,13) To date, obtaining high titers of CA through strain improvements of S. clavuligerus has primarily depended on random mutagenesis and genetic engineering of the synthesis pathway of CA. [14][15][16][17][18][19] In this study, we examined the effects of synthetic and vegetable oils on the cell growth and CA production of S. clavuligerus in a 7-liter jar fermentor.…”

mentioning

confidence: 99%

See 1 more Smart Citation

An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid inStreptomyces clavuligerus

Kim¹,

Kim²,

Shin³

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Utilization of vegetable oil in the production of clavulanic acid by Streptomyces clavuligerusATCC 27064

Cited by 33 publications

References 24 publications

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

An Approach to Strain Improvement and Enhanced Production of Clavulanic Acid inStreptomyces clavuligerus

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