Utilization of stomach content DNA to determine diet diversity in piscivorous fishes

Carreón-Martı́nez, Lucı́a B.; Johnson, Timothy B.; Ludsin, Stuart A.; Heath, Daniel D.

doi:10.1111/j.1095-8649.2011.02925.x

Cited by 115 publications

(118 citation statements)

References 38 publications

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“…The laboratory experiments showed increased identification success associated with the use of genetic tools. This pattern is consistent with that reported by Carreon et al (2011) based on three fish species. In most studies, only universal primers are used for genetic determination of prey.…”

Section: Discussionsupporting

confidence: 93%

Morphological identification and DNA barcoding used for diet analysis of gilthead seabream (Sparus aurata) in its expanding northerly range

Avignon¹,

Tastard²,

Weston³

et al. 2017

Aquat. Living Resour.

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-The gilthead seabream, Sparus aurata, is common in the Mediterranean Sea and along the Atlantic coasts of Portugal, Spain and France. Abundance of S. aurata has recently increased along the Brittany coast, showing good adaptation and acclimatisation to northern waters away from its original distribution range. The physiological adaptations (diet, reproduction, growth) of this fish to colder water could even lead to its colonisation of the English Channel. The ability to eat and digest hard prey makes this fish an important consumer of bivalves. The aim of this study was to make a preliminary evaluation of the diet of the gilthead seabream in its northern range of distribution. Prey items from stomach contents of wild adults from various sites along the East Atlantic coast of France to the English Channel were identified morphologically when it was possible, e.g. in presence of decapod appendices, shells of bivalves, or using DNA barcoding. Diet composition was analysed against sites, fish length and month of sampling using the frequency of occurrence (%F) and weight relative proportion (%W). Results showed that the diet of S. aurata was mainly composed of bivalves, malacostracans and gastropods with a huge dominance of Mytilus sp. (%F = 51.5 and %W = 40.2). This first diet analysis of individuals from the northern range of the species distribution showed its ability, as an opportunistic feeder, to find prey in newly colonised ecosystems and its preference for some organisms, especially mussels.

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Section: Discussionsupporting

confidence: 93%

Morphological identification and DNA barcoding used for diet analysis of gilthead seabream (Sparus aurata) in its expanding northerly range

Avignon¹,

Tastard²,

Weston³

et al. 2017

Aquat. Living Resour.

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“…Diet studies of these types have been applied extensively to marine taxa ranging from higher trophic levels (e.g., Deagle et al 2005;Dunn et al 2010;Méheust et al 2015) through marine birds (e.g., Deagle et al 2007;Bowser et al 2013) and fish (e.g., Moran et al 2016) to different invertebrates and lower trophic levels (e.g., Jarman et al 2002;Nejstgaard et al 2003;Blankenship and Yayanos 2005;Leal et al 2014b;Olsen et al 2014;Hu et al 2015;reviewed in Calado and Leal 2015). DNA-based approaches have also yielded breakthrough dietary analyses in complex freshwater systems (e.g., Corse et al 2010;Carreon-Martinez et al 2011;Bartley et al 2015). Most of the studies to date attest to the effectiveness of DNA-based analysis, especially when combined with morphological analysis (e.g., Casper et al 2007;Braley et al 2010;Alonso et al 2014), and they offer new insights in trophic ecology.…”

Section: Clarifying the Links Of Aquatic Food Websmentioning

confidence: 99%

The use of DNA barcodes in food web construction—terrestrial and aquatic ecologists unite!

Roslin

Majaneva

2016

Genome

103

100

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By depicting who eats whom, food webs offer descriptions of how groupings in nature (typically species or populations) are linked to each other. For asking questions on how food webs are built and work, we need descriptions of food webs at different levels of resolution. DNA techniques provide opportunities for highly resolved webs. In this paper, we offer an exposé of how DNA-based techniques, and DNA barcodes in particular, have recently been used to construct food web structure in both terrestrial and aquatic systems. We highlight how such techniques can be applied to simultaneously improve the taxonomic resolution of the nodes of the web (i.e., the species), and the links between them (i.e., who eats whom). We end by proposing how DNA barcodes and DNA information may allow new approaches to the construction of larger interaction webs, and overcome some hurdles to achieving adequate sample size. Most importantly, we propose that the joint adoption and development of these techniques may serve to unite approaches to food web studies in aquatic and terrestrial systems-revealing the extent to which food webs in these environments are structured similarly to or differently from each other, and how they are linked by dispersal.Key words: DNA barcodes, food webs, species delimitation, species identification, trophic links, ecological networks.Résumé : En brossant le tableau de qui mange qui, les réseaux trophiques livrent une description des liens qui unissent des groupes d'espèces ou de populations. Afin de déterminer comment ces réseaux sont constitués et comment ils fonctionnent, il nous faut une description de ces réseaux à différents niveaux de résolution. Les techniques de l'ADN permettent de produire des réseaux trophiques de grande résolution. Dans ce travail, les auteurs décrivent comment les techniques fondées sur l'ADN, en particulier les codes à barres de l'ADN, ont récemment été employées pour étudier la structure des réseaux trophiques chez des systèmes tant terrestres qu'aquatiques. Les auteurs soulignent comment ces techniques peuvent servir à simultanément améliorer la résolution taxonomique des noeuds d'un réseau (i.e. les espèces) ainsi que les relations entre eux (qui mange qui). Les auteurs terminent en proposant des moyens via lesquels les codes à barres et l'information tirée de l'ADN rendent possible de nouvelles approches en vue de la construction de plus grands réseaux d'interaction et permettent de surmonter des difficultés rencontrées dans l'acquisition d'échantillons de taille suffisante. Surtout, les auteurs proposent que l'adoption et le développement conjoints de ces techniques peut contribuer à unifier les approches employées dans l'étude des réseaux trophiques au sein des systèmes aquatiques et terrestres. Cela permettra de révéler l'étendue de la similarité ou de la dissimilarité dans la façon dont sont structurés les réseaux dans ces différents environnements et comment ils sont liés par la dispersion. [Traduit par la Rédaction]

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“…3 conservation concern, individuals can be sacrificed and prey tissue in the guts can be isolated and used for visual or DNA analysis (Carreon-Martinez et al, 2011). However, it…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Faeces of generalist predators as ‘biodiversity capsules’: A new tool for biodiversity assessment in remote and inaccessible habitats

2015

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Utilization of stomach content DNA to determine diet diversity in piscivorous fishes

Cited by 115 publications

References 38 publications

Morphological identification and DNA barcoding used for diet analysis of gilthead seabream (Sparus aurata) in its expanding northerly range

Morphological identification and DNA barcoding used for diet analysis of gilthead seabream (Sparus aurata) in its expanding northerly range

The use of DNA barcodes in food web construction—terrestrial and aquatic ecologists unite!

Faeces of generalist predators as ‘biodiversity capsules’: A new tool for biodiversity assessment in remote and inaccessible habitats

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