Utilization of starch effluent from a textile industry as a fungal growth supplement for enhanced α-amylase production for industrial application

Kalia, Shweta; Bhattacharya, Arghya; Prajapati, Sanjeev Kumar; Pant, Kamal K.

doi:10.1016/j.chemosphere.2021.130554

Cited by 25 publications

(11 citation statements)

References 51 publications

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“…Certain high-value compounds produced in A. niger do not accumulate sufficiently until late in the culture, and some functional proteases even take several days to show detectable activity . Because of the ability of the core–shell droplet to maintain the stable long-term growth of A.…”

Section: Discussionmentioning

confidence: 99%

Core–Shell Droplet-Based Microfluidic Screening System for Filamentous Fungi

Zhang,

Wu,

Song

et al. 2023

ACS Sens.

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Filamentous fungi are competitive hosts for the production of drugs, proteins, and chemicals. However, their utility is limited by screening methods and low throughput. In this work, a universal high-throughput system for optimizing protein production in filamentous fungi was described. Droplet microfluidics was used to encapsulate large mutant strain pools in biocompatible core−shell microdroplets designed to avoid mycelial punctures and thus sustain prolonged culture. The self-assembled split GFP was then used to characterize the secretory capacity of the strains and isolate strains with superior production titers according to the fluorescence signals. The platform was applied to optimize the α-amylase secretion of Aspergillus niger, resulting in the isolation of a strain with 2.02-fold higher secretion capacity. The system allows the analysis of >10 5 single cells per h and will facilitate ultrahigh-throughput screening experiments of filamentous fungi. This method could help identify improved hosts for the large-scale production of biotechnology-relevant proteins. This is a broadly applicable system that can be equally used in other hosts.

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Section: Discussionmentioning

confidence: 99%

Core–Shell Droplet-Based Microfluidic Screening System for Filamentous Fungi

Zhang,

Wu,

Song

et al. 2023

ACS Sens.

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“…Therefore, PW is an interesting raw material to produce sugars by enzymatic hydrolysis, using amylase as the main enzyme. The production of amylase by SsF has been studied on several raw materials such as WB, dried potato peel, dried fruit waste, rice bran, groundnut cake, coconut cake, soybean powder, bagasse, paddy husk, to name a few [ 29 , 30 ]. However, WB has shown the highest yield on amylase activity [ 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…The production of amylase by SsF has been studied on several raw materials such as WB, dried potato peel, dried fruit waste, rice bran, groundnut cake, coconut cake, soybean powder, bagasse, paddy husk, to name a few [ 29 , 30 ]. However, WB has shown the highest yield on amylase activity [ 29 , 30 ]. Amongst others, one of the main factors influencing enzyme production is the microorganism used to ferment the solid.…”

Section: Methodsmentioning

confidence: 99%

“…TMF-2, a strain isolated from dairy product (Collection of cultures of the Faculty of Technology and Metallurgy, University of Belgrade) [ 11 ]. However, Kalia et al obtained 192 U/g of α-amylase with the fungus Trichoderma reesei when WB was supplemented with salt media containing NaNO 3 (2 g/L), K 2 HPO 4 (1 g/L), MgSO 4 ·7H 2 O, (0.5 g/L), KCl (0.5 g/L), yeast extract (2.5 g/L) [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

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Production of lactic acid from pasta wastes using a biorefinery approach

Marzo-Gago

Venus

López‐Gómez

2022

Biotechnol Biofuels

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A total of 398 kt of pasta waste (PW), generated during the production process of pasta, were produced in 2021. Due to its chemical composition and practically zero cost, PW has already been studied as a raw material for the production of lactic acid (LA) through fermentations. The main objective of this article was to improve the economic viability of the process by replacing commercial enzymes, necessary for starch hydrolysis in PW, with raw enzymes also produced from wastes. Enzyme synthesis was achieved through solid-state fermentation (SsF) of wheat bran by Aspergillus awamori or Aspergillus oryzae at various moisture contents. The maximum amylase activity (52 U/g dry solid) was achieved after 2 days of fermentation with A. awamori at 60% of moisture content. After that, the enzymes were used to hydrolyse PW, reaching 76 g/L of total sugars, 65 g/L of glucose and a yield of 0.72 gglu/gds with the enzymes produced by A. awamori. Subsequently, the hydrolysate was fermented into LA using Bacillus coagulans A559, yielding 52 g/L and 49 g/L with and without yeast extract, respectively. Remarkably, compared to the process with commercial enzymes, a higher LA yield was reached when enzymes produced by SsF were added (0.80 gLA/gglu). Furthermore, the productivities between the two processes were similar (around 3.9 g/L/h) which highlights that yeast extract is not necessary when using enzymes produced by SsF.

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“…7 However, the oxidant and alkali desizings require many chemical reagents (strong alkalis and oxidants) to remove the sizes, which produces a large amount of waste water. 8,9 In addition, the reagents used in desizing can destroy the cellulose structure and cause the loss of fiber strength. 10 These methods are gradually being replaced by enzyme desizing.…”

mentioning

confidence: 99%

Differences in the desizability of starches and the mechanism of inhibiting desizing

Zhang

Baek

Yuan

et al. 2022

Textile Research Journal

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The desizing efficiencies of starches are different. However, the reasons and mechanism for this appearance are still unclear. Therefore, desizing efficiencies of starches were measured, and the reasons for their difference were analyzed. Swelling ability and degree of crystallinity of starch films were tested to conform to the relation with desizing. The size residue on the desized yarns was collected to detect the difference in the proportion of nonstarch components and the structure of hydrolyzed starch. In addition, correlation analyses were conducted between the factors affecting hydrolysis and desizing efficiency. It was found that corn starch has the lowest desizing efficiency, and the efficiency of cassava starch was the highest. The largest proportion of protein was the main reason for inhibiting the desizing. Furthermore, a higher proportion of lipid and the lower swelling ability of starch film could also be an obstacle to desizing. Removing the nonstarch components and improving the swelling ability of film may be effective methods to improve the desizing efficiency of starch.

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Utilization of starch effluent from a textile industry as a fungal growth supplement for enhanced α-amylase production for industrial application

Cited by 25 publications

References 51 publications

Core–Shell Droplet-Based Microfluidic Screening System for Filamentous Fungi

Core–Shell Droplet-Based Microfluidic Screening System for Filamentous Fungi

Production of lactic acid from pasta wastes using a biorefinery approach

Differences in the desizability of starches and the mechanism of inhibiting desizing

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