Utilization of Scenedesmus obliquus biomass as feedstock for biodiesel and other industrially important co-products: An integrated paradigm for microalgal biorefinery

Patnaik, Reeza; Mallick, Nirupama

doi:10.1016/j.algal.2015.09.009

Cited by 42 publications

(12 citation statements)

References 32 publications

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“…Lipid extraction was done following Bligh and Dyer (1959) following which the amount of lipid obtained was expressed as gL –1 . Transesterification of the extracted lipids was done following Mandal et al (2013) and analyzed using GC-MS as per the method detailed in Patnaik and Mallick (2015).…”

Section: Methodsmentioning

confidence: 99%

“…This manuscript being a sequel to the previously published paper by Patnaik and Mallick (2015), the test microalga for this part of the study has been grown under the optimized condition for designing the algal refinery. The optimized condition comprised of S. obliquus grown in N 11 medium supplemented with 0.17% acetate, 0.17% citrate and 0.4 g L –1 nitrate and incubated for a period of 9 days in a temperature controlled culture room.…”

Section: Methodsmentioning

confidence: 99%

“…The optimized condition comprised of S. obliquus grown in N 11 medium supplemented with 0.17% acetate, 0.17% citrate and 0.4 g L –1 nitrate and incubated for a period of 9 days in a temperature controlled culture room. The sequential extraction of all the components from the test microalga other than protein was done as per the methods detailed in Patnaik and Mallick (2015) whereas the protein part was extracted following the method standardized in this manuscript.…”

Section: Methodsmentioning

confidence: 99%

“…In the paper published by Patnaik and Mallick (2015), the green microalga Scenedesmus obliquus was used as a model organism to produce biodiesel and other industrially important co-products such as β-carotene, omega-3 fatty acids, glycerol and bioethanol in a sequential process in the designed algal refinery, but the use of protein present in the same microalgal biomass was not shown. Hence in this research study, for improved valorization of the S. obliquus biomass, the utilization of the leftover microalgal protein has been demonstrated by using the protein-rich algae meal as a replacement of the commercially available fish meal in the diets of the freshwater fishes.…”

Section: Introductionmentioning

confidence: 99%

“…(SAG 276-3a) biomass after which, the extracted protein has been used to formulate a protein-rich algae meal for the freshwater fishes. After completion of the fish feeding experiments, the algal refinery design shown by Patnaik and Mallick (2015) has been further enriched by addition of the protein component of the test microalga in the valorization process, eventually utilizing the major components present in S. obliquus under the optimized condition for production of industrially important products through the designed refinery.…”

Section: Introductionmentioning

confidence: 99%

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Utilization of Scenedesmus obliquus Protein as a Replacement of the Commercially Available Fish Meal Under an Algal Refinery Approach

et al. 2019

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The approach of algal refinery as a method to reduce the cost of algal biodiesel by co-production of various value-added chemicals is the most up-coming strategy suggested for the economic viability of microalgal biodiesel. This concept being relatively new and novel, abundant literature on the subject is not available although fragmented data on some feedstocks are present. The main objective of this research paper is to propose an algal refinery design through utilization of Scenedesmus obliquus biomass for production of various industrially important products. For this purpose, first a protocol was standardized for maximum extraction of protein from S. obliquus biomass. Then, different experiments were conducted for 90 days each to find out the optimum concentration of microalgal protein that can be substituted in the diets of freshwater fishes for their maximum growth. During these experiments eight different growth parameters and seven water quality parameters were tested. Results showed that the standard + whole microalgal biomass + extracted microalgal protein diet (25:25:50) was the best diet for maximum growth of the freshwater fishes. After conducting these experiments, a detailed sequential extraction process for maximum valorization of the S. obliquus biomass or in other words an algal refinery was designed. The detailed sequential process developed, yielded 0.06 g of β-carotene, 10 g of protein, 38 g (43 mL) of biodiesel, 2 g of omega-3 fatty acid, 3 g (2.4 mL) of glycerol and 18 g (23 mL) of bioethanol from 1 Kg wet (≈100 g dry) S. obliquus biomass thus converting 70% of the test microalgal biomass into biodiesel and other value-added products by using an algal refinery approach.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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