Utilization of polyvinyl butyral-zirconium alkoxide hybrid hollow tube as an enzyme immobilization carrier

Rice straw enhancing catalysis of Pseudomonas fluorescens lipase for synthesis of citronellyl acetate

“…To understand the nonaqueous stability of immobilized PFL, the RSP-PFL and RSF-PFL bioreactors were used to catalyze the transesterification of citronellol and vinyl acetate at 37 ℃ and 160 rpm for 8 h, by which the catalysis was repeated for six times via replacing reaction solution with new reactants and the relative activities of them were expressed by comparing every conversion with the conversion after the first use, which was taken as 100% relative activity [8]. After the sixth time (Fig.…”

“…In addition, the other immobilized lipases have been studied for synthesis of citronellyl acetate. Novozyme 435 usually was regarded as a better commercial immobilized lipase in synthesis of citronellyl acetate [10], but the high disturbance rate 600 rpm inevitably consumed a lot of energy and Table 1 Comparing RSP-PFL and RSF-PFL with several lipase-catalyzed reaction systems for synthesis of citronellyl acetate: composition of reaction systems, reaction conditions and reuses a Enzymes studied in this paper b native PFL super to other commercial lipases reported for the synthesis of citronellyl acetate [9] c Candida antarctica lipase B immobilized on acrylic resin [10] d Candida antarctica lipase immobilized on polyvinyl butyral-zirconium alkoxide hybrid hollow tube [8] e a biomass-bond lipase from psychrophilic Chrysosporium pannorum A-1 effectively produced by fungal submerged culture at 20 °C [62] f other compositions g the optimum conversion obtained at the condition different from that in the reuses of biomass-CPL [62] h the stirring condition by magnetic stirrer [62] i no relevant experimental; j relative activity changed from the initial 100% to the percentage value after the nth reuse k the average attenuation rate of relative activity…”

“…RSP-PFL a) the use of toluene as solvent also increased the difficulty in separation of product (Table 1). Also, the immobilized lipase was ever prepared by entrapping Candida antarctica lipase (CAL) in polyvinyl butyral-zirconium alkoxide hybrid hollow tube [8], which was called PVB-CAL by us (Table 1). PVB-CAL was used for synthesis of citronellyl acetate via esterification of citronellol and acetic acid, and the results showed that it had desired nonaqueous stability judged by the small attenuation rate of relative activity, being around 0.3% h −1 approximately equal to that of RSP-PFL and RSF-PFL.…”

“…If citronellyl acetate is synthesized by chemical catalysis, although there is increase in the yield, the high temperature and pressure usually are required, which result in generation of toxic byproduct polluted to environment, and correspondingly the product is difficultly purified [5]. Therefore, it is critical to find a more efficient, green and low-cost synthesis method, for instance enzymatic method [6,7], to prepare citronellyl acetate via esterification between citronellol and acetic acid [8] or transesterification between citronellol and vinyl acetate [9,10]. Enzymatic method is an environmentally friendly way, and can be used to produce products with high purity equal to or higher than that of natural extraction products, having high economic value [11].…”