Utilization of organic substrates during mixotrophic and heterotrophic cultivation of algae

Dvořáková-Hladká, Jiřina

doi:10.1007/bf02930672

Cited by 21 publications

(5 citation statements)

References 17 publications

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“…Both methanogenic bacteria and C . vulgaris are able to metabolize monosaccharides, several disaccharides (including cellobiose) and short‐chain oligosaccharides (Dvořáková‐Hladká 1966), and therefore are good candidates to be cultivated on the products of PASC hydrolysis. We measured the amount of reducing and total sugars in the supernatant of PASC treated with the enzyme cocktail (Figure 4, supernatant C).…”

Section: Resultsmentioning

confidence: 99%

A microalgal‐based preparation with synergistic cellulolytic and detoxifying action towards chemical‐treated lignocellulose

Benedetti

Barera

Longoni

et al. 2020

Plant Biotechnology Journal

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High-temperature bioconversion of lignocellulose into fermentable sugars has drawn attention for efficient production of renewable chemicals and biofuels, because competing microbial activities are inhibited at elevated temperatures and thermostable cell wall degrading enzymes are superior to mesophilic enzymes. Here, we report on the development of a platform to produce four different thermostable cell wall degrading enzymes in the chloroplast of Chlamydomonas reinhardtii. The enzyme blend was composed of the cellobiohydrolase CBM3GH5 from C. saccharolyticus, the b-glucosidase celB from P. furiosus, the endoglucanase B and the endoxylanase XynA from T. neapolitana. In addition, transplastomic microalgae were engineered for the expression of phosphite dehydrogenase D from Pseudomonas stutzeri, allowing for growth in non-axenic media by selective phosphite nutrition. The cellulolytic blend composed of the glycoside hydrolase (GH) domain GH12/GH5/GH1 allowed the conversion of alkaline-treated lignocellulose into glucose with efficiencies ranging from 14% to 17% upon 48h of reaction and an enzyme loading of 0.05% (w/w). Hydrolysates from treated cellulosic materials with extracts of transgenic microalgae boosted both the biogas production by methanogenic bacteria and the mixotrophic growth of the oleaginous microalga Chlorella vulgaris. Notably, microalgal treatment suppressed the detrimental effect of inhibitory byproducts released from the alkaline treatment of biomass, thus allowing for efficient assimilation of lignocellulose-derived sugars by C. vulgaris under mixotrophic growth.

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Section: Resultsmentioning

confidence: 99%

A microalgal‐based preparation with synergistic cellulolytic and detoxifying action towards chemical‐treated lignocellulose

Benedetti

Barera

Longoni

et al. 2020

Plant Biotechnology Journal

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“…Algae are ubiquitous phototrophic organisms with versatile metabolic pathways, which have been well exploited to obtain multiple products through biorefineries 8,9 ; however, the presence of cellulases and cellulolytic activities in these organisms have been poorly described. In 1966, Dvořáková‐Hladká 10 reported the presence of β‐glucosidase activity in S. obliquus , which allows it to grow using cellobiose as a substrate. In 1970, Burczyk and collaborators suggested the presence of extracellular cellulases in S. obliquus due to the observation that the cell walls accumulated in the medium by the release of autospores from the mother cells were deprived of the cellulose layer present in the daughter cells 11 …”

Section: Introductionmentioning

confidence: 99%

Molecular insight into cellulose degradation by the phototrophic green alga Scenedesmus

et al. 2023

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Lignocellulose is the most abundant natural biopolymer on earth and a potential raw material for the production of fuels and chemicals. However, only some organisms such as bacteria and fungi produce enzymes that metabolize this polymer. In this work we have demonstrated the presence of cellulolytic activity in the supernatant of Scenedesmus quadricauda cultures and we identified the presence of extracellular cellulases in the genome of five Scenedesmus species. Scenedesmus is a green alga which grows in both freshwater and saltwater regions as well as in soils, showing highly flexible metabolic properties. Sequence comparison of the different identified cellulases with hydrolytic enzymes from other organisms using multisequence alignments and phylogenetic trees showed that these proteins belong to the families of glycosyl hydrolases 1, 5, 9, and 10. In addition, most of the Scenedesmus cellulases showed greater sequence similarity with those from invertebrates, fungi, bacteria, and other microalgae than with the plant homologs. Furthermore, the data obtained from the three dimensional structure showed that both, their global structure and the main amino acid residues involved in catalysis and substrate binding are well conserved. Based on our results, we propose that different species of Scenedesmus could act as biocatalysts for the hydrolysis of cellulosic biomass produced from sunlight.

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“…However, the presence of cellulases and cellulolytic activity was poorly described in algae. In 1966, Dvořáková-Hladká et al reported the presence of β-glucosidase activity in S. obliquus , which allows it to grow using cellobiose as a substrate 10 . In 1970 Burczyk and col. reported the presence of extracellular cellulases in Scenedesmus obliquusbecause cell walls accumulated in the medium as a result of mother cells autospore release were deprived of the cellulose layer present in daughter cells.…”

Section: Introductionmentioning

confidence: 99%

Molecular insight into cellulose degradation by the phototrophic green alga Scenedesmus.

Barchiesi

Velazquez

Busi

et al. 2022

Preprint

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Lignocellulose is the most abundant natural biopolymer on earth and a potential raw material for the production of fuels and chemicals. However, only some organisms such as bacteria and fungi produce the necessary enzymes to metabolize it. In this work we detected the presence of extracellular cellulases in the genome of five species of Scenedesmus. These microalgae grow in both, freshwater and saltwater regions as well as in soils, displaying highly flexible metabolic properties. The comparison of sequences of the different cellulases with hydrolytic enzymes from other organisms by means of multi-sequence alignments and phylogenetic trees showed that these enzymes belong to the families of glycosyl hydrolases 1, 5, 9 and 10. In addition, most of these presented a greater similarity of sequence with enzymes from invertebrates, fungi, bacteria and other microalgae than with cellulases from plants; and the 3D modeling data obtained showed that both the main structures of the modeled proteins and the main amino acid residues implicated in catalysis and substrate binding are well conserved in Scenedesmus enzymes. We propose that these cellulase-producing phototrophic microorganisms could act as catalysts for the hydrolysis of cellulosic biomass fueled by sunlight.

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Utilization of organic substrates during mixotrophic and heterotrophic cultivation of algae

Cited by 21 publications

References 17 publications

A microalgal‐based preparation with synergistic cellulolytic and detoxifying action towards chemical‐treated lignocellulose

A microalgal‐based preparation with synergistic cellulolytic and detoxifying action towards chemical‐treated lignocellulose

Molecular insight into cellulose degradation by the phototrophic green alga Scenedesmus

Molecular insight into cellulose degradation by the phototrophic green alga Scenedesmus.

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