Utilization of Ocimum basilicum extracts for zinc oxide nanoparticles synthesis and their antibacterial activity after a novel combination with phage

Abdelsattar, Abdallah S.; Mohamed, Warda F. I.; Gouda, Shrouk Mohamed; Safwat, Anan; Hakim, Toka A.; El‐Shibiny, Ayman

doi:10.1016/j.matlet.2021.131344

Cited by 16 publications

(9 citation statements)

References 11 publications

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“…Furthermore, the SEM micrographs corresponding to the heatmap showed signs of cell membrane damage which might be the reason behind the antibacterial effect of the formulated Au@ AgNPs. This observation agreed with previous studies that reported cell membrane damage with various types of nanoparticles (Nisar et al 2019;Vazquez-Muñoz et al 2019;Abdelsattar et al 2022). In addition to the cell membrane damage, nanoparticles lead to protein and DNA misfolding and reactive oxygen species (ROS) production were all reported as various mechanisms of antibacterial properties of the nanoparticle (Herbin et al 2022).…”

Section: The Antibacterial Activity Of the Au@agnpssupporting

confidence: 93%

New formula of the green synthesised Au@Ag core@shell nanoparticles using propolis extract presented high antibacterial and anticancer activity

et al. 2022

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Antimicrobial alternatives such as nanoparticles are critically required to tackle bacterial infections, especially with the emerging threat of antibiotic resistance. Therefore, this study aimed to biosynthesize Au–Ag nanoparticles using propolis as a natural reducing agent and investigate their antibacterial activity against antibiotic-resistant Staphylococcus sciuri (S. sciuri), Pseudomonas aeruginosa (P. aeruginosa), and Salmonella enterica Typhimurium (S. enterica), besides demonstrating their anticancer activity in cancer cell lines. The biosynthesized Au@AgNPs were characterized using UV–Vis spectrophotometer, Transmission Electron Microscopy (TEM), Zeta potential, Dynamic Light Scattering (DLS), Fourier Transformation Infrared (FTIR), and Scanning Electron Microscopy (SEM). Moreover, the detection of antibacterial activity was assessed through disc diffusion, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), time-killing curve, and detection of cell membrane integrity via SEM. As a result, the UV–Vis spectrum revealed the formation of Au@AgNPs in a single peak between 533 and 555 nm. Furthermore, FTIR analysis confirmed nanoparticles’ green synthesis due to the presence of carbon functional groups. The formulated Au@AgNPs showed antibacterial activity against both Gram-positive and Gram-negative bacteria. The MIC and the MBC of P. aeruginosa and S. sciuri were 31.25 µg/mL. However, nanoparticles were more effective on S. enterica with MIC of 7.5 µg/mL and MBC of 15.6 µg/mL. Furthermore, the time-killing curve of the three model bacteria with the treatment was effective at 50 µg/mL. Besides, SEM of the tested bacteria indicated unintegrated bacterial cell membranes and damage caused by Au@AgNPs. Regarding the anticancer activity, the results indicated that the biosynthesized Au@AgNPs have a cytotoxic effect on HEPG2 cell lines. In conclusion, this research revealed that the green synthesized Au@AgNPs could be effective antibacterial agents against S. sciuri, P. aeruginosa, and S. enterica and anticancer agents against HEPG2.

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Section: The Antibacterial Activity Of the Au@agnpssupporting

confidence: 93%

New formula of the green synthesised Au@Ag core@shell nanoparticles using propolis extract presented high antibacterial and anticancer activity

et al. 2022

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“…Accordingly, these results indicate the instability of the formulation and forming aggregates as a part of the limitation. However, the values are similar to or higher than the previous formulations [28,40].…”

Section: Characterization Of Zn-nps Using Zeta-potentialsupporting

confidence: 74%

“…The antibacterial activity of ZnO-NPs, AuNPs@ZnO and AgNPs@ZnO preparations were evaluated at different concentrations which are 10 mg/mL, 5 mg/mL, 2.5 mg/mL, and 1.25 mg/mL. All concentrations were tested against Grampositive Staphylococcus sciuri (S. sciuri) (MW415886) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) (OL375153) and Salmonella enterica (NCTC 13,348) using agar disk diffusion method [27,28]. Briefly, bacterial cultures from each bacterium separately were spread on Tryptic Soya Agar (TSA) plates.…”

Section: Disk Diffusion Testmentioning

confidence: 99%

The Promising Antibacterial and Anticancer Activity of Green Synthesized Zinc Nanoparticles in Combination with Silver and Gold Nanoparticles

Abdelsattar

Kamel

Hussein

et al. 2023

J Inorg Organomet Polym

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Zinc nanoparticles (ZnNPs) are showing promising medical applications. However, their cytotoxicity is relatively high. This study aims to use a green synthesis technique based on the natural propolis (honeybees glue) and produce three counterparts of Zinc nanoparticles (ZnO-NPs, AuNPs@ZnO and AgNPs@ZnO). Then, the three would be analyzed for their potential antibacterial activity and cytotoxicity. The study compares the antibacterial activity and cytotoxicity of ZnO-NPs alone to its combination with gold and silver (AuNPs@ZnO and AgNPs@ZnO). The results showed that AgNPs@ZnO had contributed significantly to antibacterial activity against Gram-positive and Gram-negative bacteria. Moreover, AuNPs@ZnO and AgNPs@ZnO showed similar cytotoxicity to ZnO-NPs with higher safety. Furthermore, the Scanning and Transmission Electron Microscopes’ micrographs (SEM and TEM) displayed the biosynthesized ZnNPs that have a spherical shape with sizes ranging from 17 to 70 nm, from 45 to 75 nm, and from 22 to 73 nm for ZnONPs, ZnO-AgNPs, free AgNPs, and ZnO-AuNPs, respectively. In addition, zeta potential values for ZnONPs were 11.4 mV, while ZnO-AgNPs and ZnO-AuNPs have a higher overall charge of 13.6 mV and 23.9 mV, respectively. Furthermore, ZnNPs exhibited antibacterial activity, yet AgNPs@ZnO demonstrated the highest antibacterial action against Gram-negative and Gram-positive bacteria. In addition, cytotoxicity analyses of biosynthesized ZnNPs on three cell lines; breast cancer cell (MCF-7), liver cancer cell (HepG-2) and Human skin fibroblast (HSF) revealed that Zn-NPs in a combination could function as an anticancer agent. Therefore, there are promising medical applications for the biosynthesized ZnNPs in combinations with other metals, such as ZnO-AgNPs, as safe antibacterial and anticancer medicines. Graphical Abstract

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“…Similarly, Islam et al (2019) isolated three new Salmonella phages and found that when 10 000 and 1000 MOI was applied, they reached the Salmonella count that fell below the detection limit in milk (<10 1 CFU/ mL) for 4 °C. Abdelsattar et al (2022) reported a decrease in the number of viable bacteria after 4 h in International Journal of Food Science and Technology 2024 the presence of phages in milk at 4 °C. In addition to the biofilm removal effect of p_S3 phage, it is thought that it has protective activity in foods and can increase the shelf life of milk in the refrigerator.…”

Section: Biological Control Of Food With Phagesmentioning

confidence: 99%

Effect of phage and rhamnolipid on Salmonella Infantis biofilm removal and biological control of phage on food deterioration

Erol,

Kaskatepe

2023

Int J of Food Sci Tech

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SummaryBacteria adherence to the surface and the resulting biofilm have serious harmful effects in food. Biofilm formation in foods can lead to food deterioration and disease transfer easily. Some alternative applications are being tried in removing biofilms. Among these, biosurfactants and bacteriophages are promising owing to their low toxicity, easy availability, and high activity. In our study, the action of phage and rhamnolipid, a biosurfactant, on the biofilm of Salmonella Infantis, which is an important pathogen in food, was examined. Additionally, the efficacy of phages in the biocontrol of foods was tested. It is observed that the phage significantly reduces the bacterial concentration in milk in the first 4h at 4 °C and 25 °C and maintains its effect for 24 h at 4 °C. The p_S3 phage, rhamnolipid (RML), and p_S3 phage+RML mixture on the 48‐h biofilm decreased the biofilm mass by 44.9‐81.8%, 20.8‐81.1%, and 12‐60%, respectively. On a 1‐week‐old biofilm, the p_S3 phage, RML, and p_S3 phage+RML mixture decreased the biofilm mass by 43‐60%, 55‐72%, and 49.7‐61.7%, respectively. It is seen that the p_S3 phage and RML co‐administration on biofilm can be a biosafe application in food production facilities where it is a more effective application in removing the biofilm in long‐term exposure.

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Utilization of Ocimum basilicum extracts for zinc oxide nanoparticles synthesis and their antibacterial activity after a novel combination with phage

Cited by 16 publications

References 11 publications

New formula of the green synthesised Au@Ag core@shell nanoparticles using propolis extract presented high antibacterial and anticancer activity

New formula of the green synthesised Au@Ag core@shell nanoparticles using propolis extract presented high antibacterial and anticancer activity

The Promising Antibacterial and Anticancer Activity of Green Synthesized Zinc Nanoparticles in Combination with Silver and Gold Nanoparticles

Effect of phage and rhamnolipid on Salmonella Infantis biofilm removal and biological control of phage on food deterioration

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