Utilization of methyltrioctylammonium chloride as new ionic liquid in pretreatment of sugarcane bagasse for production of cellulase by novel thermophilic bacteria

Ejaz, Uroosa; Muhammad, Shoaib; Hashmi, Imran Ali; Ali, Firdous Imran; Sohail, Muhammad

doi:10.1016/j.jbiotec.2020.04.013

Cited by 29 publications

(16 citation statements)

References 21 publications

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“…Qadir et al 16 . and Ejaz et al 17 . also reported that alkaline pretreatment results in physical changes in SB structure.…”

Section: Resultsmentioning

confidence: 97%

“…2). 17 The band at 1618 cm −1 in the spectrum of UTB was assigned to CC bond which represents the aromatic structure of lignin, whereas the band at 2364 cm −1 represents CO 2 stretching 15 . The presence of carbohydrate and lignin was further confirmed by looking at the region between 1300 and 1500 cm −1 in the spectrum of UTB that exhibited a high molecular coupling, involving a superposition of several modes of vibration 18 ; while the band at around 1200 cm −1 indicated the presence of hemicellulose and lignin 19 …”

Section: Resultsmentioning

confidence: 99%

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Supporting role of lignin in immobilization of yeast on sugarcane bagasse for continuous pectinase production

Ejaz

Sohail

2020

J Sci Food Agric

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BACKGROUND Lignocellulosic wastes are pretreated prior to their utilization in fermentation processes. Such pretreatment also alters the topological features of the substrates, and therefore the suitability of pretreated waste as immobilization matrix for microbial cells needs investigation. RESULTS In this study, the effect of chemical pretreatment of sugarcane bagasse (SB) for its subsequent utilization as a matrix to immobilize a pectinolytic yeast, Geotrichum candidum AA15, was evaluated using cell retention, concentration of immobilized cells, immobilization efficiency, scanning electron microscopy and Fourier transform infrared spectroscopy of the substrate and pectinase titers obtained after recycling. The results revealed that untreated SB is more efficient for immobilization with higher values of cell retention and pectinase productivity (99.78%) retained for up to six production cycles. It was deduced that removal of lignin by pretreatment negatively influenced the ability of SB to support cell adhesion, as lignin acts as a sealing agent that provides strength to the substrate. CONCLUSIONS The strategy of utilizing SB as immobilization matrix was found effective at the laboratory scale as it improved pectinase production and may be investigated further for large‐scale and cost‐effective production. © 2020 Society of Chemical Industry

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“…Qadir et al 16 . and Ejaz et al 17 . also reported that alkaline pretreatment results in physical changes in SB structure.…”

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Supporting role of lignin in immobilization of yeast on sugarcane bagasse for continuous pectinase production

Ejaz

Sohail

2020

J Sci Food Agric

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“…Moreover, the recyclability and reusability of IL is yet another advantage by which, the higher cost of IL can be compensated. Various techniques have been reported for recycling IL such as rotary evaporation [15], crystallization, liquid extraction, vacuum distillation, ion exchange nanofiltration, adsorption and salting out [40]. Ejaz et al [41] recycled methyltrioctylammonium chloride for six times by evaporating ethanol from it retaining 70% xylanase productivity by recylcled IL pretreated SB.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an ammonium-based IL, methyltrioctylammonium chloride, was used in the current study. It is a water insoluble salt with low melting point and less viscosity [15]. The pretreatment of SB with this IL has been reported recently for the production of cellulases from thermophilic bacteria [15], therefore, it was tested to investigate its effectiveness in subsequent xylan extraction from SB.…”

Section: Introductionmentioning

confidence: 99%

“…It is a water insoluble salt with low melting point and less viscosity [15]. The pretreatment of SB with this IL has been reported recently for the production of cellulases from thermophilic bacteria [15], therefore, it was tested to investigate its effectiveness in subsequent xylan extraction from SB. Considering the important component of lignocellulose (LC), xylan recovery from SB is a strategy to use hemicellulose component with commercial applicability.…”

Section: Introductionmentioning

confidence: 99%

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Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

et al. 2020

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Background Lignin in sugarcane bagasse (SB) hinders its utilization by microorganism, therefore, pretreatment methods are employed to make fermentable components accessible to the microbes. Multivariate analysis of different chemical pretreatment methods can aid to select the most appropriate strategy to valorize a particular biomass. Results Amongst methods tested, the pretreatment by using sodium hydroxide in combination with methyltrioctylammonium chloride, an ionic liquid, (NaOH+IL) was the most significant for xylanase production by Bacillus aestuarii UE25. Investigation of optimal levels of five significant variables by adopting Box-Behnken design (BBD) predicted 20 IU mL− 1 of xylanase and experimentally, a titer of 17.77 IU mL− 1 was obtained which indicated the validity of the model. The production kinetics showed that volumetric productivity of xylanase was much higher after 24 h (833.33 IU L− 1 h− 1) than after 48 h (567.08 IU L− 1 h− 1). The extracted xylan from SB induced more xylanase in the fermentation medium than pretreated SB or commercially purified xylan. Nuclear Magnetic Resonance, Fourier transform infrared spectroscopy and scanning electron microscopy of SB indicated removal of lignin and changes in the structure of SB after NaOH+IL pretreatment and fermentation. Conclusion Combined pretreatment of SB with alkali and methyltrioctylammonium chloride appeared better than other chemical methods for bacterial xylanase production and for the extraction of xylan form SB.

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Screening, characterization, and optimization of the fermentation conditions of a novel cellulase‐producing microorganism from soil of Qinghai–Tibet Plateau

Zhao,

Zhang,

Qin

et al. 2024

Biotech and App Biochem

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Cellulases play an important role in the bioconversion of lignocellulose. Microorganisms found in extreme environments are a potentially rich source of cellulases with unique properties. Due to the uniqueness of the environment, the abundant microbial resources in the Qinghai–Tibet Plateau (QTP) are worth being explored. The aim of this study was to isolate and characterize an acidic, mesophilic cellulase‐producing microorganism from QTP. Moreover, the fermentation conditions for cellulase production were optimized for future application of cellulase in the development of lignocellulose biomass. A novel cellulase‐producing strain, Penicillium oxalicum XC10, was isolated from the soil of QTP. The cellulase produced by XC10 was a mesophilic cellulase that exhibited good acid resistance and some cold‐adaptation properties, with maximum activity at pH 4.0 and 40°C. Cellulase activity was significantly enhanced by Na+ (p < 0.05) and inhibited by Mg2+, Ca2+, Cu2+, and Fe3+ (p < 0.05). After optimization, maximum cellulase activity (8.56 U/mL) was increased nearly 10‐fold. Optimal fermentation conditions included an inoculum size of 3% (v/v) in a mixture of corn straw (40 g/L), peptone (5 g/L), and Mg2+ (4 g/L), at pH 4.0, 33°C, and shaking at 200 rpm. The specific properties of the P. oxalicum XC10 cellulase suggest the enzyme may serve as an excellent candidate for the bioconversion and utilization of lignocellulose biomass generated as agricultural and food‐processing wastes.

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Utilization of methyltrioctylammonium chloride as new ionic liquid in pretreatment of sugarcane bagasse for production of cellulase by novel thermophilic bacteria

Cited by 29 publications

References 21 publications

Supporting role of lignin in immobilization of yeast on sugarcane bagasse for continuous pectinase production

Supporting role of lignin in immobilization of yeast on sugarcane bagasse for continuous pectinase production

Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production

Screening, characterization, and optimization of the fermentation conditions of a novel cellulase‐producing microorganism from soil of Qinghai–Tibet Plateau

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