Utilization of &lt;i&gt;Cypermethrin&lt;/i&gt; by bacteria isolated from irrigated soils

Shamsuddeen, U; Inuwa, AB

doi:10.4314/bajopas.v6i2.4

Cited by 3 publications

(3 citation statements)

References 4 publications

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“…Lozano and Dussán [ 55 ] also described the potential of Lysinibacillus sphaericus to be used in bioremediation of heavy metals. Sphingobacterium and Streptococcus , also only detected in resistant mosquitoes in this study, are bacterial genera known to degrade pyrethroid insecticides such as cypermethrin [ 56 – 58 ]. Bacteria belonging to the genera Streptococcus and Rubrobacter have been categorized as core microbiota of the digestive system of Anopheles culicifacies [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Western Kenyan Anopheles gambiae showing intense permethrin resistance harbour distinct microbiota

Omoke

Kipsum

Otieno

et al. 2021

Malar J

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Background Insecticide resistance poses a growing challenge to malaria vector control in Kenya and around the world. Following evidence of associations between the mosquito microbiota and insecticide resistance, the microbiota of Anopheles gambiae sensu stricto (s.s.) from Tulukuyi village, Bungoma, Kenya, with differing permethrin resistance profiles were comparatively characterized. Methods Using the CDC bottle bioassay, 133 2–3 day-old, virgin, non-blood fed female F1 progeny of field-caught An. gambiae s.s. were exposed to five times (107.5 µg/ml) the discriminating dose of permethrin. Post bioassay, 50 resistant and 50 susceptible mosquitoes were subsequently screened for kdr East and West mutations, and individually processed for microbial analysis using high throughput sequencing targeting the universal bacterial and archaeal 16S rRNA gene. Results 47 % of the samples tested (n = 133) were resistant, and of the 100 selected for further processing, 99 % were positive for kdr East and 1 % for kdr West. Overall, 84 bacterial taxa were detected across all mosquito samples, with 36 of these shared between resistant and susceptible mosquitoes. A total of 20 bacterial taxa were unique to the resistant mosquitoes and 28 were unique to the susceptible mosquitoes. There were significant differences in bacterial composition between resistant and susceptible individuals (PERMANOVA, pseudo-F = 2.33, P = 0.001), with presence of Sphingobacterium, Lysinibacillus and Streptococcus (all known pyrethroid-degrading taxa), and the radiotolerant Rubrobacter, being significantly associated with resistant mosquitoes. On the other hand, the presence of Myxococcus, was significantly associated with susceptible mosquitoes. Conclusions This is the first report of distinct microbiota in An. gambiae s.s. associated with intense pyrethroid resistance. The findings highlight differentially abundant bacterial taxa between resistant and susceptible mosquitoes, and further suggest a microbe-mediated mechanism of insecticide resistance in mosquitoes. These results also indicate fixation of the kdr East mutation in this mosquito population, precluding further analysis of its associations with the mosquito microbiota, but presenting the hypothesis that any microbe-mediated mechanism of insecticide resistance would be likely of a metabolic nature. Overall, this study lays initial groundwork for understanding microbe-mediated mechanisms of insecticide resistance in African mosquito vectors of malaria, and potentially identifying novel microbial markers of insecticide resistance that could supplement existing vector surveillance tools.

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Section: Discussionmentioning

confidence: 99%

Western Kenyan Anopheles gambiae showing intense permethrin resistance harbour distinct microbiota

Omoke

Kipsum

Otieno

et al. 2021

Malar J

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“…According to this author, the use of insecticide molecules and less aggressive formulations must be pursued for all those using such technologies to increase food and energy production, without compromising sustainability of the agricultural ecosystems. Different results were found by Shamsuddeen and Inuwa (2013) which reported slow growth of bacteria Pseudomonas aeruginosa up to four hours after application of Cipermetrine and after this period there was rapid growth, showing that this bacteria used the Cipermetrine as carbon source up to certain limits, which thereby stimulating their growth. This way, demostrating that can serve as a tool for environmental mitigation.…”

Section: Resultsmentioning

confidence: 69%

Impact of xenobiotics on microbial activity in soil cultivated with forage cactus Opuntia ficus-indica

Rios¹,

Oliveira²,

Oliveira³

et al. 2016

Afr. J. Agric. Res.

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Changes on soil microbial activity may be triggered by different management approaches and the study of the effects of such changes on xenobiotics, of non-target populations, may represent a valuable strategy to evaluate their environmental risk potential. The objective of the present study was to evaluate the effect of Phytosanitary control measures over microbial activity and genetic variability of bacteria in soil cultivated under the forage cactus Opuntia ficus-indica. The experiment was performed at Caetés region in Pernambuco, Brazil. Three days after the application of the xenobiotics (water (control); detergent + sodium hypochlorite; Neem oil; Methomyl and Thiamethoxam + Lambdacyhalotrin), soil samples (0-20 cm) were collected and transported to laboratory. Respirometry, density of bacterial population and morphologic and genetic variability of bacteria were evaluated with molecular techniques, using BOX-PCR in a completely randomized statistical design. Regarding to respirometry, the amount of CO 2 released from the soil samples was greater within the plots where the insecticide Thiamethoxam + Lambda-cyhalotrin was applied, when compared with control. Soil treatments with only water and water + sodium hypochlorite showed the highest population densities (0.96 and 0.94 × 10 2 CFU.g-1 , respectively). Concerning to morphological characteristics (color), there was prevalence of white color colonies, with a little visual phenotypic variability. However, the use of molecular techniques revealed high genetic variability among the white colored colonies evaluated, demonstrating the importance of more detailed studies on the effects of xenobiotics on soil microbiota prior to its use of recommendation.

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“…Lozano and Dussán [55] also described the potential of Lysinibacillus sphaericus to be used in bioremediation of heavy metals. Sphingobacterium and Streptococcus, also only detected in resistant mosquitoes in this study, are bacterial genera known to degrade pyrethroid insecticides such as cypermethrin [56–58]. Bacteria belonging to the genera Streptococcus and Rubrobacter have been categorized as core microbiota of the digestive system of Anopheles culicifacies [59].…”

Section: Discussionmentioning

confidence: 99%

Western Kenyan Anopheles gambiae s.s. showing intense permethrin resistance harbor distinct microbiota

Omoke

Kipsum

Otieno

et al. 2020

Preprint

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BackgroundInsecticide resistance poses a growing challenge to malaria vector control in Kenya and around the world. Following evidence of associations between the mosquito microbiota and insecticide resistance, we comparatively characterized the microbiota of An. gambiae s.s. from Tulukuyi village, Bungoma, Kenya, with differing permethrin resistance profiles.MethodsUsing the CDC bottle bioassay, 133 2-3 day-old, virgin, non-blood fed female F1 progeny of field-caught An. gambiae s.s. were exposed to five times (107.5μg/ml) the discriminating dose of permethrin. Post bioassay, 50 resistant and 50 susceptible mosquitoes were subsequently screened for kdr East and West mutations, and individually processed for microbial analysis using high throughput sequencing targeting the universal bacterial and archaeal 16S rRNA gene.Results47% of the samples tested (n=133) were resistant, and of the 100 selected for further processing, 99% were positive for kdr East and 1% for kdr West. Overall, 84 bacterial taxa were detected across all mosquito samples, with 36 of these shared between resistant and susceptible mosquitoes. A total of 20 were unique to the resistant mosquitoes and 28 were unique to the susceptible mosquitoes. There were significant differences in bacterial composition between resistant and susceptible individuals (F=2.33, P=0.001), with presence of Sphingobacterium, Lysinibacillus and Streptococcus (all known pyrethroid-degrading taxa), and the radiotolerant Rubrobacter, being significantly associated with resistant mosquitoes. On the other hand, the presence of Myxococcus, was significantly associated with susceptible mosquitoes.ConclusionThis is the first report of distinct microbiota in An. gambiae s.s. associated with intense pyrethroid resistance. The findings highlight differentially abundant bacterial taxa between resistant and susceptible mosquitoes, and further suggest a microbe-mediated mechanism of insecticide resistance in mosquitoes. Our results also indicate fixation of the kdr East mutation in this mosquito population, precluding further analysis of its associations with the mosquito microbiota, but presenting the hypothesis that any microbe-mediated mechanism of insecticide resistance would be likely of a metabolic nature. Overall, this study lays initial groundwork for understanding microbe-mediated mechanisms of insecticide resistance in African malaria vectors, and potentially identifying novel microbial markers of insecticide resistance that could supplement existing vector surveillance tools.

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Utilization of <i>Cypermethrin</i> by bacteria isolated from irrigated soils

Cited by 3 publications

References 4 publications

Western Kenyan Anopheles gambiae showing intense permethrin resistance harbour distinct microbiota

Western Kenyan Anopheles gambiae showing intense permethrin resistance harbour distinct microbiota

Impact of xenobiotics on microbial activity in soil cultivated with forage cactus Opuntia ficus-indica

Western Kenyan Anopheles gambiae s.s. showing intense permethrin resistance harbor distinct microbiota

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