Utilization of lignocellulosic waste by the edible mushroom, Pleurotus

Hadar, Yitzhak; Kerem, Zohar; Gorodecki, Barbara; Ardon, Orly

doi:10.1007/bf00129083

Cited by 29 publications

(14 citation statements)

References 36 publications

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“…with threonine in the WRF Lentinus tigrinus [44] whereas histidine, aspartic and glutamic acids were main amino acids in the WRF Pleurotus ostreatus mycelium [45]. Nevertheless, amino acid composition remains strain and substrate dependent but also depends on culture conditions [46].…”

Section: N-containing (N) and Unspecific Compounds (Un)mentioning

confidence: 99%

Pyrolysis-GC–MS to assess the fungal pretreatment efficiency for wheat straw anaerobic digestion

Rouches

Dignac

Zhou

et al. 2017

Journal of Analytical and Applied Pyrolysis

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Cost-effective and environment-friendly pretreatments, such as fungal pretreatments, are required for anaerobic digestion of lignocellulosic biomass particularly because the amount of methane produced is often limited by the lignin content. Anaerobic digestibility is estimated using a Biochemical Methane Potential (BMP) test, which lasts several weeks. Since the Py-GC–MS technique is considered to be a rapid method for obtaining information on various organic components, its suitability for the study of fungal pretreatment efficiency in anaerobic digestion was investigated here, to our knowledge for the first time. In this goal, mycelium of the white-rot fungi Polyporus brumalis BRFM 985, untreated wheat straw and straw pretreated with different fungal strains and under different conditions were analysed with Py-GC–MS. In the mycelium pyrolysate, diverse compounds, often considered as unspecific, probably derive from proteins (toluene, etc.). A strong presence of tyrosine and phenylalanine among the fungus amino acids was also suspected. As for pretreated straw samples, a correlation was observed between the amount of fungal biomass determined by qPCR (used as a reference method) and the sum of relative areas of toluene, styrene and ethylbenzene in the pyrograms, showing that it is feasible to estimate the fungal biomass amount on pretreated straws using Py-GC–MS. In addition, the H/L-Py (Holocelluloses/Lignin) ratio, determined by dividing the sums of areas of pyrolysis compounds that have a polysaccharide (PS) and lignin (LIG) origin, was correlated to the BMP values of pretreated straws, thus showing that the pretreatment efficiency can be rapidly estimated with Py-GC–MS in the tested conditions

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Section: N-containing (N) and Unspecific Compounds (Un)mentioning

confidence: 99%

Pyrolysis-GC–MS to assess the fungal pretreatment efficiency for wheat straw anaerobic digestion

Rouches

Dignac

Zhou

et al. 2017

Journal of Analytical and Applied Pyrolysis

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“…In natural substrates, cosubstrates are obtained by enzymatic hydrolysis of polysaccharides (24). However, some fungi (the white-rot fungi) degrade lignin faster than they degrade polysaccharides, and delignified polysaccharides accumulate, thereby upgrading the waste (7,24,31).…”

mentioning

confidence: 99%

Effect of manganese on preferential degradation of lignin by Pleurotus ostreatus during solid-state fermentation

Kerem

Hadar

1995

Appl Environ Microbiol

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Practical utilization of the polysaccharides in the lignocellulosic complex is limited because of the high lignin content of the complex. In this study we focused on the effect of Mn on lignin and cellulose biodegradation during solid-state fermentation by the edible mushroom Pleurotus ostreatus. Preferential degradation of lignin was enhanced by the addition of Mn(II) to cotton stalks at concentrations ranging from 30 to 620 g of Mn per g. This effect was most apparent when we compared mineralization rates of [ 14 C]lignin with mineralization rates of [ 14 C]cellulose. Enhanced selectivity was also observed when we analyzed residual organic matter at the end of the fermentation period by using crude fiber analysis. The cellulose fraction in the original material was 1.8 times larger than the cellulose fraction of lignin. The cellulose/lignin ratio increased during 32 days of solid-state fermentation from 2.5 in the control to 3.3 following the addition of Mn to the medium. The in vitro digestibility value for fermented cotton stalks was 53% of the dry matter. Addition of 600 g of Mn per g to the cotton stalks resulted in a digestibility value of 65.4%. Enhancement of preferential lignin degradation could be the result of either increased activity of the ligninolytic enzymes or production of Mn(III), which might preferentially degrade aromatic structures in the lignocellulosic complex.

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“…La MnP depolimeriza compuestos fenólicos y fenoles metilados en lignina sintética, lo que indica que este sistema podría ser el que se presenta en la depolimerización de lignina in vivo (Kirk & Cullen, 1998). La MnP no es una enzima fuertemente oxidante y en consecuencia incapaz de atacar estructuras recalcitrantes no fenólicas predominantes en la lignina, sin embargo, si puede oxidar las estructuras fenólicas más reactivas (estas conforman aproximadamente el 10% de la lignina), estas divisiones dan origen a un grado limitado de lignolisis a través de división aril y otras vías de degradación, debido a los pequeños radicales que produce y su capacidad de penetrar la lignina, es el facilitador para el ataque posterior de otras enzimas más grandes y con mayor poder oxidante como la lignino peroxidasa (LiP) o Lacasa (Hadar et al, 2012). En este trabajo la enzima MnP en estudio pertenece al organismo Pleurotus ostreatus con el número de acceso GenBank: AAA84397.1 formato tomado del banco de datos de National Center for Biotechnology Information (NCBI) el cual se encuentra constituido por una secuencia de 363 aminoácidos para ser modelada por homología por el programa bioinformático PMV que nos permite generar una imagen ( Fig.…”

Section: Manganeso Peroxidasaunclassified

Herramientas bioinformáticas usadas en el estudio de enzimas fenoloxidasas del género Pleurotus

et al. 2018

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El análisis bioinformático permite relacionar, predecir y analizar un conjunto de proteínas de interés que comparten áreas conservadas en común a través de un computador (in silico). Este análisis bioinformático se realiza a partir de la secuencia de bases de datos, dominios y de regiones clave que conforman la función catalítica de una proteína. La secuencia de aminoácidos proporciona una predicción correspondiente a las regiones conservadas. Posteriormente las secuencias de aminoácidos se acoplan de manera ordenada por su función y estructura, mismas que generan proyecciones espaciales de estructuras terciarias que permiten el reconocimiento de secuencias claves para mutaciones coordinadas. La bioinformática en las enzimas fenoloxidasa como lacasa, manganeso peroxidasa y decolorante peroxidasa permite observar estructuras proteicas, cadenas alfa y beta, así como los iones responsables de la catálisis. Las técnicas in silico juegan un papel importante en áreas como la medicina, la biotecnología y la genómica en beneficio de la salud humana. La técnica de secuenciación de ADN identifica organismos por medio de secuencias de aminoácidos tomadas de bancos de datos para elaborar proteínas molde, alineamiento de secuencias, modelamientos por homología y la validación o identificación de organismos.

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Utilization of lignocellulosic waste by the edible mushroom, Pleurotus

Cited by 29 publications

References 36 publications

Pyrolysis-GC–MS to assess the fungal pretreatment efficiency for wheat straw anaerobic digestion

Pyrolysis-GC–MS to assess the fungal pretreatment efficiency for wheat straw anaerobic digestion

Effect of manganese on preferential degradation of lignin by Pleurotus ostreatus during solid-state fermentation

Herramientas bioinformáticas usadas en el estudio de enzimas fenoloxidasas del género Pleurotus

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