Utilization of isolated marine mussel cells as an in vitro model to assess xenobiotics induced genotoxicity

Zhang, Y.F.; Chen, S.Y.; Qu, Moyuan; Adeleye, Adedayo O.; Di, Yanan

doi:10.1016/j.tiv.2017.05.018

Cited by 21 publications

(5 citation statements)

References 82 publications

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“…Similarly, field studies revealed an increase of DNA damage in haemocytes of zebra mussels inhabiting polluted sites, which correlated with the level of contaminants in water [ 27 ]. In the present study in vivo zinc exposure induced a statistically significant increase of DNA strand breaks compared to controls, confirming data from the literature evaluated in different sentinel species [ 46 , 47 , 48 ], being Zn induced DNA damage also known to occur in marine mussels’ cells [ 18 , 49 , 50 ].…”

Section: Resultssupporting

confidence: 91%

Cellular Responses Induced by Zinc in Zebra Mussel Haemocytes. Loss of DNA Integrity as a Cellular Mechanism to Evaluate the Suitability of Nanocellulose-Based Materials in Nanoremediation

et al. 2021

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Zinc environmental levels are increasing due to human activities, posing a threat to ecosystems and human health. Therefore, new tools able to remediate Zn contamination in freshwater are highly recommended. Specimens of Dreissena polymorpha (zebra mussel) were exposed for 48 h and 7 days to a wide range of ZnCl2 nominal concentrations (1–10–50–100 mg/L), including those environmentally relevant. Cellulose-based nanosponges (CNS) were also tested to assess their safety and suitability for Zn removal from freshwater. Zebra mussels were exposed to 50 mg/L ZnCl2 alone or incubated with 1.25 g/L of CNS (2 h) and then removed by filtration. The effect of Zn decontamination induced by CNS has been verified by the acute toxicity bioassay Microtox®. DNA primary damage was investigated by the Comet assay; micronuclei frequency and nuclear morphological alterations were assessed by Cytome assay in mussels’ haemocytes. The results confirmed the genotoxic effect of ZnCl2 in zebra mussel haemocytes at 48 h and 7-day exposure time. Zinc concentrations were measured in CNS, suggesting that cellulose-based nanosponges were able to remove Zn(II) by reducing its levels in exposure waters and soft tissues of D. polymorpha in agreement with the observed restoration of genetic damage exerted by zinc exposure alone.

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Section: Resultssupporting

confidence: 91%

Cellular Responses Induced by Zinc in Zebra Mussel Haemocytes. Loss of DNA Integrity as a Cellular Mechanism to Evaluate the Suitability of Nanocellulose-Based Materials in Nanoremediation

et al. 2021

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“…In addition, a ten-fold higher frequency of apoptotic cells (APO) was found in ZnCl 2 -exposed mussels' gill cells compared to the controls, and no further differences were observed among mussels exposed after CNS treatment and those exposed to CNS only (Figure 6b). cells of M. galloprovincialis [56,57]. However, upon CNS treatment, the DNA integrity of mussels' gill cells was comparable to that of the controls and the mussels exposed to CNS alone.…”

Section: Cellular Bioassaysmentioning

confidence: 84%

“…ZnCl 2 's ability to induce DNA strand breaks has been documented in the circulating and tissue cells of M. galloprovincialis [56,57]. However, upon CNS treatment, the DNA integrity of mussels' gill cells was comparable to that of the controls and the mussels exposed to CNS alone.…”

Section: Cellular Bioassaysmentioning

confidence: 99%

“…The main pathway of Zn(II) uptake in Mytilus is through the gills, from which it is transported to the digestive system via the hemolymph [47][48][49][50]. Zn-induced biological responses/effects have been already well identified in marine mussels, both at cellular and tissue levels, and described in detail [51][52][53][54][55][56][57]. Being the first organs involved in Zn(II) uptake in bivalves, the gills and mantle are the main targets of Zn toxicity in mussels [58].…”

Section: Introductionmentioning

confidence: 99%

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Effect-Based Approach to Assess Nanostructured Cellulose Sponge Removal Efficacy of Zinc Ions from Seawater to Prevent Ecological Risks

et al. 2020

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To encourage the applicability of nano-adsorbent materials for heavy metal ion removal from seawater and limit any potential side effects for marine organisms, an ecotoxicological evaluation based on a biological effect-based approach is presented. ZnCl2 (10 mg L−1) contaminated artificial seawater (ASW) was treated with newly developed eco-friendly cellulose-based nanosponges (CNS) (1.25 g L−1 for 2 h), and the cellular and tissue responses of marine mussel Mytilus galloprovincialis were measured before and after CNS treatment. A control group (ASW only) and a negative control group (CNS in ASW) were also tested. Methods: A significant recovery of Zn-induced damages in circulating immune and gill cells and mantle edges was observed in mussels exposed after CNS treatment. Genetic and chromosomal damages reversed to control levels in mussels’ gill cells (DNA integrity level, nuclear abnormalities and apoptotic cells) and hemocytes (micronuclei), in which a recovery of lysosomal membrane stability (LMS) was also observed. Damage to syphons, loss of cilia by mantle edge epithelial cells and an increase in mucous cells in ZnCl2-exposed mussels were absent in specimens after CNS treatment, in which the mantle histology resembled that of the controls. No effects were observed in mussels exposed to CNS alone. As further proof of CNS’ ability to remove Zn(II) from ASW, a significant reduction of >90% of Zn levels in ASW after CNS treatment was observed (from 6.006 to 0.510 mg L−1). Ecotoxicological evaluation confirmed the ability of CNS to remove Zn from ASW by showing a full recovery of Zn-induced toxicological responses to the levels of mussels exposed to ASW only (controls). An effect-based approach was thus proven to be useful in order to further support the environmentally safe (ecosafety) application of CNS for heavy metal removal from seawater.

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“…Tissue and xenobiotic specific formation of DNA strand‐breaks and MN was observed in freshly isolated cells from hemolymph, gill, and digestive gland of mussels ( M. galloprovincialis ) after short term in vitro exposure to different concentrations of metals (Zn, Cu, Cd, and Pb) and PCBs. Combined treatment of metal and PCBs mixtures induce formation of DNA damage even at low concentrations, (Zhang et al ). In this study, however, associations between PCBs and GT indicators were not revealed.…”

Section: Discussionmentioning

confidence: 99%

Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

Rybakovas

Arbačiauskas

Markovskienė

et al. 2019

Environ and Mol Mutagen

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European inland waters are under continuous threat of anthropogenic pollution. Determination of background level of biomarker response and subsequent classification of the impact increases the applicability of results. In the current study, we evaluate the range of chemical contamination by measuring the concentrations of metals, polycyclic aromatic hydrocarbons and polychlorinated biphenyls, and the levels of environmental genotoxicity by using the micronuclei and nuclear bud tests in bivalve mussels of the major Lithuanian rivers. Second, we aimed to evaluate the association between chemical contamination and genotoxicity biomarker responses. Finally, we set to determine the background level of genotoxic effects. Such value (summed frequency of MN and NB) was assessed—6‰. On that basis, we develop a scale of potential genotoxic impact and perform ranging sites into five categories. The results clearly indicate the existence of significant differences in the levels of chemical pollution and genotoxicity in different sites. Increased levels of studied parameters were assessed at the areas affected by municipal and industrial effluents, road runoff, combustion products, and in the area contaminated by accidental spillage. On the other hand, downstream decrease of contamination level, presumably associated with biological degradation and photochemical oxidation, were also observed. Genotoxicity parameters were associated with PAH and metal concentrations measured in mussel tissues as well as in sediments. Results also indicate that in situ genotoxicity assessment performed in the areas affected by long‐term contamination of municipal origin might be not sufficiently precise. Study highlights the necessity to combine genotoxicity assessment with chemical analysis. Environ. Mol. Mutagen. 61:338–354, 2020. © 2019 Wiley Periodicals, Inc.

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Utilization of isolated marine mussel cells as an in vitro model to assess xenobiotics induced genotoxicity

Cited by 21 publications

References 82 publications

Cellular Responses Induced by Zinc in Zebra Mussel Haemocytes. Loss of DNA Integrity as a Cellular Mechanism to Evaluate the Suitability of Nanocellulose-Based Materials in Nanoremediation

Cellular Responses Induced by Zinc in Zebra Mussel Haemocytes. Loss of DNA Integrity as a Cellular Mechanism to Evaluate the Suitability of Nanocellulose-Based Materials in Nanoremediation

Effect-Based Approach to Assess Nanostructured Cellulose Sponge Removal Efficacy of Zinc Ions from Seawater to Prevent Ecological Risks

Contamination and genotoxicity biomarker responses in bivalve mussels from the major Lithuanian rivers

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