Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts

Ribeiro, A. R.; Nascimento, Vladimir Pinheiro do; Cardoso, Marisa; Santos, Luciana Ruschel dos; Rocha, Sílvio Luis da Silveira

doi:10.1590/s1517-83822002000400012

Cited by 9 publications

(8 citation statements)

References 8 publications

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“…In Brazil, Salmonella Enteritidis has been one of the most prevalent serovars isolated in human infections (12) as well as in nonhuman sources, which include broiler carcasses (22), foodstuffs, poultry flocks, environment, sewage, water, animal feed, chiller water, other animals, viscera, stools, unknown sources (24), and raw broilers parts (21 The strains were isolated using the conventional method for detection of Salmonella, recommended by the Brazilian Ministry of Agriculture and Supply (9). Briefly, twenty-five grams of skin and muscle, collected under aseptic conditions, were taken from each broiler carcass and homogenized in 225 mL of Bufferd Peptone Water (BPW)(Merck AG, Darmstadt, Germany), and incubated at 37ºC for 24h (pre-enrichment).…”

mentioning

confidence: 99%

Antibiotic resistance in Salmonella Enteritidis isolated from broiler carcasses

Cardoso¹,

Ribeiro²,

Santos³

et al. 2006

Braz. J. Microbiol.

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Eighty Salmonella Enteritidis strains isolated from broiler carcasses between May 1995 and April 1996 in the State of Rio Grande do Sul, Brazil, were tested for antibiotic susceptibility using the disk diffusion method. Resistance to colistin, novobiocin, erythromycin and tetracycline was observed in 100% of the isolates. The strains showed intermediate resistance at different levels to kanamycin (1.25%), enrofloxacin (3.75%), neomycin (3.75%), fosfomycin (20%), sulphonamides (86.25%) and nitrofurantoin (90%). Resistance to ciprofloxacin, norfloxacin, gentamicin, polymyxin B, sulphametrim and sulphazotrim was not found. Since resistance to antibiotics especially those introduced in the last decades, was detected, it is recommended that their use must be based on the results of resistance tests or minimum inhibitory concentration tests.

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mentioning

confidence: 99%

Antibiotic resistance in Salmonella Enteritidis isolated from broiler carcasses

Cardoso¹,

Ribeiro²,

Santos³

et al. 2006

Braz. J. Microbiol.

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“…Table 1, conventional culture and immunomagnetic separation methods helped isolation of Salmonella bacteria in seven samples. According to the formula described in Boer and Beumer [21] , the sensivities of the methods used are found to be as 71.4% for conventional culture method, and 42.8% for IMS. Based on the data and calculations given above, conventional culture method was found to be 28.6% more sensitive than immunomagnetic separation method.…”

Section: Resultsmentioning

confidence: 99%

“…Anti-Salmonella paramagnetic beads are anti-Salmonella antibodies that bound covalently bacteria surface. The immunomagnetic separation was accomplished starting from 1 mL the pre-enrichment broth was transferred to an Eppendorf tube containing 20 µL of the immunomagnetic microbeads coated with anti-Salmonella [20,21] . The tubes were shaken on a Dynal® MX3 sample mixer (Dynal®, Norway) at room temperature for 10 min.…”

Section: Immunomagnetic Separation Methods (Ims)mentioning

confidence: 99%

Comparison of Different Methods for the Detection of Salmonella spp. in Minced Meat Samples

Ozer¹,

Kimiran-Erdem²

2013

Kafkas Univ Vet Fak Derg

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The presence of Salmonella spp. in minced meat that is consumed in nine different sites of Istanbul is evaluated by using conventional culture (ISO 6579:2002), immunomagnetic separation (IMS) and fluorescent in situ hybridization (FISH) methods. Salmonella spp. was isolated from five of 50 (20%) minced meat with ISO 6579 method, and three of 50 (6%) minced meat using IMS method. Bacteria isolated from both ISO and IMS methods were identified as Salmonella choleraesuis ssp. arizonae and Salmonella spp. The presence of Salmonella spp. was determined from 37 of 50 (74%) minced meat by using FISH method. In the current study, it has been shown that ISO 6579 method was found more to be susceptible than IMS method for determining presence of Salmonella spp., FISH method is the best method to determine the presence of Salmonella spp. Even if the quick determination of the epidemics of international importance occured as a result of the contamination by pathogens derivated from foods, the results of the use of new methods should be supported by the conventional culture method. Keywords: Conventional culture method (ISO 6579 reference method), Fluorescent in situ hybridization (FISH) method,Immunomagnetic separation method, Minced meat, Salmonella Kıyma Örneklerinde Salmonella spp. Tespitinde Farklı Yöntemlerin Karşılaştırılması Özetİstanbul'un dokuz farklı semtinde tüketime sunulan kıyma örneklerinde geleneksel kültür yöntemi (ISO), immünomanyetik ayırma (İMA) ve floresanlı yerinde hibritleme (FISH) yöntemleri kullanılarak Salmonella cinsi bakterilerin varlığı tespit edilmiştir. İncelenen 50 kıyma örneğinin, geleneksel kültür yöntemi ile 5' inden (20%), İMA yöntemi ile 3' ünden (6%) Salmonella cinsi bakteri izole edilmiştir. Gerek geleneksel kültür yöntemi gerekse de İMA yönteminde besiyeri üzerinde üreyen kolonilerden elde edilen izolatlar Salmonella choleraesuis ssp. arizonae ve Salmonella spp. olarak tanımlanmıştır. İncelenen 50 kıyma örneğinin 37'sinde (74%) ise FISH yöntemi ile Salmonella cinsi bakterilerin varlığı tespit edilmiştir. Çalışmamızda Salmonella cinsi bakterilerin varlığını belirlemede geleneksel kültür yönteminin, İMA yöntemine göre daha duyarlı bir yöntem olduğu, FISH yönteminin ise Salmonella cinsi bakterilerin varlığını belirlemede en iyi yöntem olduğu tespit edilmiştir. Gıda kaynaklı patojenlerle kontaminasyon sonucunda ortaya çıkan uluslararası önemdeki salgınların hızlı tespiti ne kadar önemli olsa da, uygulanan yeni yöntemlerin sonuçlarının geleneksel kültür yöntemi ile desteklenmesi gerekmektedir.

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“…In contrast, other studies have reported a poor performance of the method that combines IMS with plating on selective agar for Salmonella detection in chicken samples (13,14). The use of different enrichment protocols before IMS can be the cause of such contrasting results, since it is well known that selective agents of enrichment broths and incubation time and temperature are factors that affect decisively the analysis outcome (6).…”

Section: Resultsmentioning

confidence: 99%

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Conceição¹,

Moreira²,

Ramos³

et al. 2008

Braz. J. Microbiol.

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The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S. Enteritidis were analyzed immediately after contamination and after 24h of refrigeration using three enrichment protocols. The detection limit of the IMS-plating procedure after standardization with pure culture was about 2x10 CFU/mL. The protocol using non-selective enrichment for 6-8h, selective enrichment for 16-18h and a post-enrichment for 4h gave the best results of S. Enteritidis detection by IMS-plating in experimentally contaminated meat. IMSplating using this protocol was compared to the standard culture method for salmonellae detection in naturally contaminated chicken cuts and yielded 100% sensitivity and 94% specificity. The method developed using in house prepared magnetic microespheres for IMS and plating in selective agar was able to diminish by at least one day the time required for detection of Salmonella in chicken products by the conventional culture method.

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Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts

Cited by 9 publications

References 8 publications

Antibiotic resistance in Salmonella Enteritidis isolated from broiler carcasses

Antibiotic resistance in Salmonella Enteritidis isolated from broiler carcasses

Comparison of Different Methods for the Detection of Salmonella spp. in Minced Meat Samples

Detection of salmonella sp in chicken cuts using immunomagnetic separation

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