Utilization of hydrolysate from saccharified sugarcane bagasse for phosphatases production

Ejaz, Uroosa; Sohail, Muhammad; El‐Bahy, Zeinhom M.; Salim, Mohamed A.

doi:10.1007/s13399-022-02828-z

Cited by 5 publications

(3 citation statements)

References 49 publications

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“…In this study, the biologically pretreated SB was fermented by the thermophilic bacterium N. sedimentimangrovi UE25, which resulted in 4.57 IU mL −1 of endoglucanase and 1.81 IU mL −1 of β ‐glucosidase. This bacterial strain has been reported previously to produce cellulase using chemically pretreated SB; 22,47,48 however, here we report compatibility of biological pretreatment with the consistent cellulase production.…”

Section: Resultssupporting

confidence: 45%

Application of Trametes pubescens for dye removal and biological pretreatment of sugarcane bagasse

Abbas,

Ejaz,

Sohail

et al. 2024

Biofuels Bioprod Bioref

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Sugarcane bagasse (SB) is an abundantly available agrowaste that is used extensively as a substrate for the production of different industrial enzymes. It is a renewable resource, rich in fermentable sugars. However, the presence of lignin makes it difficult for microbes to degrade it and so it is difficult to utilize it. Keeping in mind the role of white rot fungi in lignin degradation, Trametes pubescens MB89 was used in this study for the delignification of SB with the simultaneous production of laccase. The fungus was also used for the degradation of malachite green dye. A Box–Behnken design (BBD) was used to optimize variables affecting solid state fermentation of SB for laccase production. The leftover SB after fermentation was used for cellulase production by the thermophilic bacterium Neobacillus sedimentimangrovi UE25. The BBD depicted optimized conditions as 60% moisture, particle size 100 μ, 10 days’ incubation period, 450 μM CuSO4, and a temperature of 30 °C. The laccase titers of 1271 IU mL−1 were obtained under optimized conditions. Neobacillus sedimentimangrovi UE25 produced 4.574 IU mL−1 of endoglucanase and 1.812 IU mL−1 of β‐glucosidase by utilizing fungal pretreated SB. Scanning electron microscopy and Fourier transform infrared spectroscopy confirmed the impact of fungal cultivation on SB. Pores were observed after microbial pretreatment and there were changes in the morphological characteristics such as the pith and fiber of SB. To test the ability of laccase to break down aromatic dyes, we grew the fungus T. pubescens MB89 in a nutrient‐rich medium supplemented with malachite green dye. The fungus was able to remove 73.68% of the malachite green dye from the medium in 24 h. This shows that T. pubescens MB89 has a range of potential applications for environmental and industrial purposes.

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Section: Resultssupporting

confidence: 45%

Application of Trametes pubescens for dye removal and biological pretreatment of sugarcane bagasse

Abbas,

Ejaz,

Sohail

et al. 2024

Biofuels Bioprod Bioref

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“…Indeed, RSM has been used to optimize production of pectinase and other enzymes from different microbial strains. [ 40,45,46 ]…”

Section: Resultsmentioning

confidence: 99%

“…Indeed, RSM has been used to optimize production of pectinase and other enzymes from different microbial strains. [40,45,46] Pambi and Musonge [47] also emphasized that coefficient of determination (R 2 ) must be deliberated given the fact that it measures the overall performance of a model. Mahapatra and coworkers [48] and Brahmi et al [49] of variable dependence.…”

Section: Statistical Optimization Of Pectinase Productionmentioning

confidence: 99%

Utilization of wild Cressa cretica biomass for pectinase production from a halo‐thermotolerant bacterium

Hassan

Ejaz

Rashid

et al. 2023

Biotechnology Journal

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Halophytes are the native inhabitants of saline environment. Their biomass can be considered as a potential substrate for the production of microbial enzymes. This study was intended at feasible utilization of a halophytic biomass, Cressia cretica, for pectinase production using a halo‐ and thermo‐tolerant bacterium, Bacillus vallismortis MH 10. The data from fractionation of the C. cretica biomass revealed presence of 17% pectin in this wild biomass. Seven different factors (temperature, agitation, pH, inoculum size, peptone concentration, substrate concentration, and incubation time) affecting pectinase production using C. cretica were assessed through a statistical tool, Plackett–Burman design. Consequently, two significant factors (incubation time and peptone concentration) were optimized using the central composite design. The strain produced 20 IU mL−1 of pectinase after 24 h under optimized conditions. The enzyme production kinetics data also confirmed that 24 h is the most suitable cultivation period for pectinase production. Fourier transform infrared spectroscopy and scanning electron microscopy of C. cretica biomass ascertained utilization of pectin and structural changes after fermentation. The purification of pectinase by using DEAE column yielded specific activity and purification fold of 88.26 IU mg−1 and 3.2, respectively. The purified pectinase had a molecular weight of >65 kDa. This study offers prospects of large‐scale production of pectinase by halotolerant strain in the presence of economical and locally grown substrate that makes the enzyme valuable for various industrial operations.

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Unveiling the genomic potential of a novel thermostable glycoside hydrolases producing Neobacillus sedimentimangrovi UE25

Ejaz

Saleem

Rashid

et al. 2023

Antonie van Leeuwenhoek

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Utilization of hydrolysate from saccharified sugarcane bagasse for phosphatases production

Cited by 5 publications

References 49 publications

Application of Trametes pubescens for dye removal and biological pretreatment of sugarcane bagasse

Application of Trametes pubescens for dye removal and biological pretreatment of sugarcane bagasse

Utilization of wild Cressa cretica biomass for pectinase production from a halo‐thermotolerant bacterium

Unveiling the genomic potential of a novel thermostable glycoside hydrolases producing Neobacillus sedimentimangrovi UE25

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