Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium

Busman, Mark

doi:10.3390/jof3030043

Cited by 11 publications

(3 citation statements)

References 18 publications

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“…Our analysis did not show the production by A1021B of 8-O-methylbostrycoidin, a polyketide pigment which has been reported in association with FA [48]. Furthermore, no trichothecenes were detected in any cultivation condition.…”

Section: Metabolome Analysiscontrasting

confidence: 79%

The Shifting Mycotoxin Profiles of Endophytic Fusarium Strains: A Case Study

et al. 2019

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Fusarium species are known to establish manifold interactions with wild and crop plants ranging from pathogenicity to endophytism. One of the key factors involved in the regulation of such relationships is represented by the production of secondary metabolites. These include several mycotoxins, which can accumulate in foodstuffs causing severe health problems to humans and animals. In the present study, an endophytic isolate (A1021B), preliminarily ascribed to the Fusarium incarnatum-equiseti species complex (FIESC), was subjected to biochemical and molecular characterization. The metabolomic analysis of axenic cultures of A1021B detected up to 206 compounds, whose production was significantly affected by the medium composition. Among the most representative products, fusaric acid (FA), its derivatives fusarinol and 9,10-dehydro-FA, culmorin and bikaverin were detected. These results were in contrast with previous assessments reporting FIESC members as trichothecene rather than FA producers. However, molecular analysis provided a conclusive indication that A1021B actually belongs to the species Fusarium babinda. These findings highlight the importance of phylogenetic analyses of Fusarium species to avoid misleading identifications, and the opportunity to extend databases with the outcome of metabolomic investigations of strains from natural contexts. The possible contribution of endophytic strains in the differentiation of lineages with an uneven mycotoxin assortment is discussed in view of its ensuing impact on crop productions.

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Section: Metabolome Analysiscontrasting

confidence: 79%

The Shifting Mycotoxin Profiles of Endophytic Fusarium Strains: A Case Study

et al. 2019

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“…HPLC-MS analyses were conducted in positive electrospray ionization mode to detect mycotoxins. HPLC-MS comparisons of ion mass and elution time with purified standards were used to identify the metabolites (Busman et al, 2012;Busman, 2017). Limits of quantitation for aurofusarin, BEA, enniatin B (ENNB), enniatin B1 (ENNB1), enniatin A (ENNA), and enniatin A1 (ENNA1) were 1 ng/µl.…”

Section: Mycotoxin Analysismentioning

confidence: 99%

Five‐year survey uncovers extensive diversity and temporal fluctuations among fusarium head blight pathogens of wheat and barley in Brazil

Pereira¹,

Ward

Ponte

et al. 2020

Plant Pathology

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We conducted a five‐year survey (2011–2015) of barley and wheat fields in Paraná state, Brazil, obtaining 754 Fusarium isolates from spikes with fusarium head blight (FHB)‐symptoms. Multilocus genotyping and TEF‐1α gene sequence analyses confirmed the dominance of the F. graminearum species complex (FGSC, 75.7%), but F. poae (11.5%), as well as F. avenaceum and related members of the F. tricinctum species complex (FTSC, 8.1%) appeared as substantial contributors to FHB. Within the FGSC, F. graminearum of the 15‐ADON genotype was dominant (63%), followed by F. meridionale of the NIV genotype (23.1%), F. cortaderiae of the NIV (7%) or 3‐ADON (2.6%) genotypes, and F. austroamericanum (3.8%) of the 3‐ADON genotype. Substantial variation in pathogen composition was observed across years, with F. poae and F. meridionale frequencies significantly elevated in some years. Most F. poae strains produced DAS, diANIV, and butenolide, but not neosolaniol, T‐2, or HT‐2. All FTSC species produced moniliformin. Enniatin production was widespread among FTSC species, with the single F. acuminatum strain found to be the strongest producer of enniatins. Our findings confirm FGSC as a major contributor to FHB and expand considerably our knowledge of the presence, frequency, and conditions under which other pathogens may emerge, altering the spectrum of toxins that may accumulate in grain.

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“…HPLC-MS analyses were conducted in positive electrospray ionization mode to detect mycotoxins. HPLC-MS comparisons of ion mass and elution time with purified standards were used to identify the metabolites (Busman et al ., 2012;Busman, 2017). Limits of quantitation for aurofusarin, beauvericin, enniatin B, enniatin B1, enniatin A and enniatin A1 were 1 ng/µl.…”

Section: Mycotoxin Analysismentioning

confidence: 98%

Five-year Survey Uncovers Extensive Diversity and Temporal Fluctuations Among Fusarium Head Blight Pathogens of Wheat and Barley in Brazil

Pereira¹,

Ward²,

Ponte³

et al. 2020

Preprint

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We conducted a five-year survey (2011–2015) of barley and wheat fields in Paraná state, Brazil, analyzing 754 Fusarium isolates from Fusarium head blight (FHB)-symptomatic spikes. Multilocus genotyping and TEF-1α gene sequence analyses confirmed the dominance of the F. graminearum species complex (75.7%), but F. poae (11.5%) as well as F. avenaceum and related members of the F. tricinctum species complex (FTSC, 8.1%) appeared as substantial contributors to FHB. Within the FGSC, F. graminearum of the 15-ADON genotype was dominant (63%), followed by F. meridionale of the NIV genotype (23.1%), F. cortaderiae of the NIV (7%) or 3-ADON (2.6%) genotypes, and F. austroamericanum (3.8%) of the 3-ADON genotype. Substantial variation in pathogen composition was observed across years, with F. poae and F. meridionale frequencies significantly elevated in some years. Most F. poae strains produced DAS, diANIV and butenolide, but not Neosolaniol, T-2 or HT-2. All FTSC species produced moniliformin. Enniatin production was widespread among FTSC species, with the single F. acuminatum strain found to be the strongest producer of enniatins. Our findings confirm FGSC as a major contributor to FHB and expand considerably our knowledge of the presence, frequency and conditions under which other pathogens may emerge altering the spectrum of toxins that may accumulate in grain.

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Utilization of High Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry for Characterization of 8-O-methylbostrycoidin Production by Species of the Fungus Fusarium

Cited by 11 publications

References 18 publications

The Shifting Mycotoxin Profiles of Endophytic Fusarium Strains: A Case Study

The Shifting Mycotoxin Profiles of Endophytic Fusarium Strains: A Case Study

Five‐year survey uncovers extensive diversity and temporal fluctuations among fusarium head blight pathogens of wheat and barley in Brazil

Five-year Survey Uncovers Extensive Diversity and Temporal Fluctuations Among Fusarium Head Blight Pathogens of Wheat and Barley in Brazil

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