Utilization of frozen–thawed epididymal ram semen to preserve genetic diversity in Scrapie susceptible sheep breeds

Ehling, Christine; Rath, D.; Struckmann, C.; Frenzel, Antje; Schindler, Lothar; Niemann, Heinrich

doi:10.1016/j.theriogenology.2006.07.003

Cited by 37 publications

(32 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…De la misma forma comparando con material espermático colectado de los epidídimos de animales post mortem, como es del ovino, vacuno y ciervo ibérico, los investigadores reportan motilidades a la descongelación del 35 al 70% (Kaabi et al 2003;Ehling et al 2006;Herold et al 2004;Martins et al 2007;Garde y col. 1998;Soler et al 2003;Soler et al 2005;Fernandez-Santos et al 2006), estas motilidades totales son superiores, comparados con las motilidades del reproductor 1 de 24.68% y para el reproductor 2 de 27.31%, encontrados en el presente estudio.…”

Section: Discusión Prueba De Fertilidad In-vitro De Los Espermatozoidunclassified

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Durand¹,

Aragón²,

Guerra³

2016

Rev. Investig. Altoandin. - RIA -

View full text Add to dashboard Cite

KEY WORDS:Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de alpacas (Vicugna pacos)Viability in vitro and invivo of sperm frozen/thawed from alpaca (Vicugna pacos) vas deferens El objetivo del presente estudio fue evaluar la viabilidad in-vitro e in-vivo de los espermatozoides procedente de los conductos deferentes de alpacas. Se utilizaron 2 reproductores como donadores de espermatozoides con desviación del conducto deferente. Los espermatozoides colectados se sometieron a la congelación y descongelación con el dilutor Triladyl®. Durante el procesamiento de los espermatozoides se evaluaron la motilidad y la prueba hipo-osmotica. En la inseminación se evaluó la proporción de gestaciones producidas. Los resultados fueron los siguientes: La motilidad de los espermatozoides para los reproductores 1 y 2 fueron: a los 37°C 65.16 y 63.37% sin diferencia (P>0.05), al enfriamiento (5°C) 52.63 y 48.31% similares (P>0.05) ya la descongelación 24.51 y 33.18% mostraron diferencia (P<0.05). A la prueba hipo-osmótica fueron; a los 37°C 51.55 y 52.98% similares (P>0.05), Al enfriamiento (5°C) 46.67 y 55.93% mostraron diferencia (P<0.05) y a la descongelación 20.06 y 32.18% con diferencia (P<0.05). Las gestaciones fueron: Con espermatozoides frescos diluidos 36.36% (4/11), con espermatozoides descongelados 25.00% (5/20) y con monta 54.54% (6/11), siendo similares (P>0.05). En conclusión los espermatozoides procedentes de los conductos deferentes soportan el congelamiento y a la inseminación artificial producen gestaciones.The aim of this study was to evaluate the in-vitro and in-vivo viability of sperm from the vas deferens alpacas. 2 males as sperm donors were used to offset the vas deferens. The collected sperm cells were subjected to freezing and thawing with dilutor Triladyl®. During processing of sperm motility and hypo-osmotic test they were evaluated. Insemination in the proportion of pregnancies produced was evaluated. The results were as follows: The sperm motility of males 1 and 2 were: at 37 ° C 65.16 and 63.37% with no difference (P> 0.05), cooling (5 ° C) 52.63 and 48.31% similar (P > 0.05) and thawing 24.51 and 33.18% showed no difference (P <0.05). The hypo-osmotic test were; at 37 ° C 51.55 and 52.98 similar percentage (P> 0.05), and cooling 55.93% 46.67 showed no difference (P <0.05) and thawing 20.06 and 32.18% with difference (P <0.05). Pregnancies were diluted with fresh sperm 36.36% (4/11), with defrosted sperm 25.00% (5/20) and 54.54% mounts (6/11), being similar (P> 0.05). In conclusion sperm from the vas deferens withstand freezing and insemination are able to produce pregnancies.

show abstract

Section: Discusión Prueba De Fertilidad In-vitro De Los Espermatozoidunclassified

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Durand¹,

Aragón²,

Guerra³

2016

Rev. Investig. Altoandin. - RIA -

View full text Add to dashboard Cite

show abstract

“…Results in the current study are similar to those achieved by Fournier-Delpech et al (1979) who found no significant difference between pregnancy rates of ejaculated and epididymal ram spermatozoa following laparoscopic intrauterine insemination. Similarly, Ehling et al (2006) andÁ lvarez et al (2012) achieved pregnancy rates as high as 87 and 55.8% respectively, when frozen-thawed epididymal ram spermatozoa were inseminated by intrauterine laparoscopic AI.…”

Section: Cervicalmentioning

confidence: 94%

“…Epididymal spermatozoa are fertile when used for ICSI (human: (Silber et al 1995), IVF (Songsasen & Leibo 1998, Stout et al 2012) and even following intrauterine insemination (Fournier-Delpech et al 1979, Hori et al 2005, Ehling et al 2006, Monteiro et al 2011. Moreover, when inseminated in vivo, epididymal spermatozoa have usually achieved pregnancy rates similar to those of ejaculated spermatozoa (Fournier-Delpech et al 1979, Ehling et al 2006, Monteiro et al 2011. Such normal fertility of spermatozoa which have never come into contact with seminal plasma raises the question of whether exposure to seminal plasma from the accessory sex glands is a biological requirement vital for the in vivo function and fertility of spermatozoa or whether this substance is largely superfluous to reproductive success.…”

Section: Introductionmentioning

confidence: 99%

Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Rickard¹,

Pini²,

Soleilhavoup³

et al. 2014

Reproduction

View full text Add to dashboard Cite

Seminal plasma purportedly plays a critical role in reproduction, but epididymal spermatozoa are capable of fertilisation following deposition in the uterus, calling into question the biological requirement of this substance. Through a combination of direct observation of spermatozoa in utero using probe-based Confocal Laser Endomicroscopy, in vivo assessment of sperm fertility and in vitro analysis of various sperm functional parameters, this study investigated the role of seminal plasma in spermatozoa transit through the cervix of the ewe. Following deposition in the cervical os, epididymal spermatozoa previously exposed to seminal plasma displayed an enhanced ability to traverse the cervix as evidenced by both significantly higher pregnancy rates and numbers of spermatozoa observed at the utero-tubal junction when compared with epididymal spermatozoa not previously exposed to seminal plasma. The beneficial effect of seminal plasma on sperm transport was clearly localised to transit through the cervix as pregnancy rates of spermatozoa deposited directly into the uterus were unaffected by exposure to seminal plasma. This phenomenon was not explained by changes to sperm motion characteristics, as seminal plasma had no effect on the motility, kinematic parameters or mitochondrial membrane potential of spermatozoa. Rather, in vitro testing revealed that seminal plasma improved the ability of epididymal spermatozoa to penetrate cervical mucus recovered from ewes in oestrus. These results demonstrate that the survival and transport of ram spermatozoa through the cervix of the ewe is not linked to their motility or velocity but rather the presence of some cervical penetration trait conferred by exposure to seminal plasma.

show abstract

“…Nowadays concrete examples of application of post-mortem sperm recovery from the epididymis are available for conservation of endangered species [37] and in constructing semen banks [38][39][40]. Preservation of epididymal sperm from accidental dead or slaughtered animals has been reported in several domestic and wild species as boar [41], cattle [42,43], goat [44], ibex [45,46], red deer [47] and sheep [38,48]. However epididymal sperm extraction sometimes is not easily practicable in marginal areas, due to the lack of facilities and expertise near the farming areas of local breeds.…”

Section: Ex Situ Conservation Strategiesmentioning

confidence: 99%

Conservation of endangered animals: From biotechnologies to digital preservation

Pizzi¹,

Caroli²,

Landini³

et al. 2013

View full text Add to dashboard Cite

In the recent years, the number of endangered animals, both referred to livestock and wild species, has grown enormously. The "livestock" term refers to animals domesticated for producing commodities for man such as food, fiber and draught. Livestock biodiversity is integral to our culture, history, environment, and economy. Thousands of livestock breeds have evolved over time to suit particular environments and farming systems. Conservation and analyses of these genetic resources rely on demographic characterization and correct breeding schemes. In addition, molecular genetic studies allow to identify and monitor the genetic diversity within and across breeds and to reconstruct their evolution history. The conservation of livestock variability is also a crucial element in order to preserve and valorise specific nutritional and nutraceutical properties of animal products. Efficient ex situ and in situ conservation strategies, as well as the creation of bio-banks and specific biotechnological and bioinformatics tools for genetic analyses and digital preservation, are obligatory requirements in order to implement an appropriate action for the conservation of animal biodiversity. The main issues concerning different species are summarised, with particular reference to the livestock biodiversity still existing. Some examples of ex situ conservation strategies, which mainly refer to cryoconservation of semen, ova, embryos or tissues, developed in Italy, are presented, and the different actions in defense of Animal Genetic Resources (AnGR) developed within the European Community are illustrated. Interestingly, the same strategies for biological and digital analyses and preservation of livestock biodiversity can be exported to wild endangered animals in order to plan a correct conservation and repopulation of the species. Furthermore, the European Union has set up the guidelines to safeguard the biodiversity and to combat the extinction of animal species, and has made the protection of biodiversity and ecosystems one of the main objectives of the Sixth Environment Action Programme.

show abstract

Utilization of frozen–thawed epididymal ram semen to preserve genetic diversity in Scrapie susceptible sheep breeds

Cited by 37 publications

References 13 publications

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Viabilidad in vitro e in vivo de los espermatozoides congelados/descongelados del conducto deferente de Alpacas (vicugna pacos)

Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Conservation of endangered animals: From biotechnologies to digital preservation

Contact Info

Product

Resources

About