Utilization of food waste hydrolysate for microbial lipid and protein production byRhodosporidium toruloidesY2

Abstract

BACKGROUND Food waste has great potential for biotech processing but most waste is subject to traditional disposal methods, losing its value. To alleviate economic and environmental concerns, the hydrolysate of food waste could be utilized for biolipid and protein production by Rhodosporidium toruloides Y2. RESULTS The biomass, lipid production and protein production reached 32.1 g L−1, 7.3 g L−1 and 7.0 g L−1, respectively. The composition of fatty acids and amino acids indicated that R. toruloides Y2 grown in food waste hydrolysate had the potential to be feedstock for biolipid and microbial protein production. Model prediction using the Logistic, Luedeking‐Piret equation and modified Luedeking‐Piret equation could estimate the growth, lipid production and reducing sugar utilization of R. toruloides Y2 in food waste hydrolysate. CONCLUSION The alternative proposed for converting food waste into microbial biomass is applicable. Food waste hydrolysate is suitable for lipid and protein production by R. toruloides Y2, without any additional nutrition or detoxification procedure. © 2016 Society of Chemical Industry

Platform Study on the Development of a Nondetoxified Rice Straw Hydrolysate to Its Application in Lipid Production from Mortierella alpina

“…A survey of lipid production on switch grass hydrolysate, prepared with a higher solid loading (20% (w/v)) and sulfuric acid concentration (0.936% (v/v)), compared to the present study, by different yeasts of oleaginous Yarrowia clade, revealed the highest lipid content of 37.9% in Yarrowia lipolytica . In food waste acid hydrolysate (1.5% (v/v) sulfuric acid and a 1:2 solids:liquid loading ratio), Rhodosporidium toruloides accumulated 22.9% CDW as lipids . With wheat straw as lignocellulosic feedstock, C. curvatus endured both detoxified and nondetoxified hydrolysate and exhibited a higher lipid content in NDLH (33.5%), versus that observed in DLH (22.1%), which is analogous to current observations .…”

“…34 In food waste acid hydrolysate (1.5% (v/v) sulfuric acid and a 1:2 solids:liquid loading ratio), Rhodosporidium toruloides accumulated 22.9% CDW as lipids. 35 With wheat straw as lignocellulosic feedstock, C. curvatus endured both detoxified and nondetoxified hydrolysate and exhibited a higher lipid content in NDLH (33.5%), versus that observed in DLH (22.1%), which is analogous to current observations. 17 On diluted raw corn cob hydrolysate, Cryptococcus sp.…”

Effect of carbon and nitrogen source concentrations on the growth and lipid accumulation of yeastTrichosporon oleaginosusin continuous and batch culture

“…Considerable savings in microbial lipid production could be achieved by using inexpensive agro and industrial residues for growing microorganisms and lipid accumulation. Oleaginous yeasts could be cultivated on different low‐cost substrates including waste glycerol from biodiesel production, lignocellulosic biomass (hydrolysates from corn stover, corn cobs and wheat straw), raw materials from the food industry (olive pomace oil, stearin, N‐acetylglucosamine from shrimp processing waste), wastewaters (cheese whey, wastewaters from confectionary industries, olive mill wastewater, palm oil mill effluent, and sewage sludge), food waste hydolysate, molasses (sugar cane molasses waste and sugar beet molasses), organic acids and waste spent yeast from the brewing industry . Considerable research work has been done on studying the effect of culture conditions as well as carbon and nitrogen sources impact on the growth and lipid synthesis in oleaginous microorganisms mostly during batch or fed‐batch culture .…”

Lipid Production from Amino Acid Wastes by the Oleaginous Yeast Rhodosporidium toruloides

“…In fact, AAs can be envisioned as organic amines; once the nitrogen atom is released from the AA, the residual carbon skeleton can be readily converted into pyruvate or an intermediate of the citric acid cycle, thus fueling cellular metabolism [13]. Previously, food waste hydrolysates were evaluated for lipid and protein production by R. toruloides Y2 [14]. So far, AA wastes have not been used alone for microbial lipid production, likely because oleaginous microorganisms normally accumulate lipids under nitrogen limitation [15], while the catabolism of AA naturally generates a relatively nitrogen-rich environment.…”