Utilization of foliar fertilizer as an alternative medium for enlargement of Vanda orchid plantlets before acclimatization

Abstract

This study aims to obtain an appropriate and inexpensive alternative media combination for the growth of Vanda hybrid orchids prior to acclimatization. Experiment using Factorial Randomized Block Design. The first factor is the type of media (Vacin -Went and foliar fertilizer ) and the second factor is the addition of organic matter (coconut water composition, sweet corn composition, tomato composition, BAP, NAA and without organic matter or PGR). The results showed that the interaction of treatment on plant height was only seen at 18 weeks after treatment, while the number of leaves could be seen earlier at 16 weeks after treatment. The independent effect of organic matter and PGR on the number of leaves has been seen since the age of the plant 10 weeks after treatment, while the plant height was only seen 16 weeks after treatment. Independent treatment of growing media had no effect on height and number of leaves up to 18 weeks after planting. The use of foliar fertilizer media with the type of organic material composition being tested can be suggested as an alternative growth medium for plantlet growth, not significantly different from vacin-went with BAP media.

Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”

“…Similar results have been reported regarding the success of using of compound fertilizers. For examples, NPK 10-55-10 media plus 10% coconut water for in vitro seed germination of Dendrobium antennatum [8], NPK 32-10-10 NPK medium containing micro nutrients for the growth and development of PLBs of Eulophia graminea orchid [9]. On the other hand, the use of Half-MS strength medium also resulted in the best growth and development of PLBs in Dendrobium var.…”

“…Murashige and Skoog (MS) medium, both in full strength [4][5][6] or in half strength [7] performed as the best medium for different orchids. In addition, compound foliar fertilizers can be used for the propagation of orchid plants, such as Dendrobium antennatum [8] and Vanda orchid plantlets [9]. The results may be comparable to Murashige and Skoog media [10,11].…”

Pertumbuhan Planlet Anggrek Dendrobium (Dendrobium sp) dengan Penambahan Ekstrak Nabati Ke Dalam Media Alternatif Subkultur

“…Menurut (Ghorbani et al, 2021) ekstrak nabati dapat menggatikan senyawa yang terkandung di dalam media kultur jaringan seperti zat perangsang tumbuh, fitohormon serta unsur hara yang dibutuhkan oleh planlet. Menurut (Kasutjianingati et al, 2022) Pada subkultur anggrek yang ditanam secara in vitro, ekstrak nabati dan pupuk daun dapat digunakan sebagai pengganti media Murashige & Skoog dan Vacint & Went. Penelitian ini bertujuan untuk mengetahui dampak penambahan bahan konsentrat nabati yang berbeda ke dalam media alternatif subkultur jaringan pada plantlet anggrek dendrobium.…”

“…Pupuk daun yang memiliki kandungan unsur hara N yang tinggi akan memacu pertambahan tinggi, jumlah daun, jumlah akar dan berat berangkasan basah pada planlet anggrek Dendrobium. Menurut (Kasutjianingati et al, 2022) pertumbuhan plantlet dapat dibantu oleh stimulasi sintesis sitokinin dengan menyediakan N. Menurut (Purnamasari et al, 2020) penggunaan pupuk daun dengan konsentrasi yang tepat sebagai bahan media subkultur memiliki nutrisi yang dapat menambah tinggi planlet anggrek dendrobium. Menurut (Inkiriwang et al, 2016) air kelapa mengandung zat aktif seperti sitokinin yang mempengaruhi metabolisme asam nukleat dan sintesa protein yang mempengaruhi pembelahan sel.…”

Multiplication of Dendrobium Sp Orchid Somatic Embries Using In-Vitro Concentrations of MgSO4 and Myo-Inositol in Murashige and Skoog Media

“…Yusnita (2003) suggests that tissue culture techniques are plant propagation techniques by growing plant parts, whether in the form of cells, tissues or organs under aseptic conditions in-vitro (Lone et al, 2020). This technique is characterized by aseptic culture conditions, the use of artificial culture media with complete nutritional content and ZPT (growth regulators) (Kasutjianingati et al, 2022), as well as culture room conditions with controlled temperature and lighting. The success of plant propagation with tissue culture is largely determined by the media used, one of which is Murashige and Skoog (MS) media.…”