Utilization of Extracorporeal Membrane Oxygenation Alleviates Intestinal Ischemia–Reperfusion Injury in Prolonged Hemorrhagic Shock Animal Model

Zhao, Liang; Luo, Lin; Chen, Jinjin; Xiao, Juan; Jia, Weikun; Ye, Xiao

doi:10.1007/s12013-014-0121-3

Cited by 18 publications

(14 citation statements)

References 37 publications

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“…These complications will result in shock, hemorrhage, thrombosis, and hemolysis Ogaki et al, 2014). Therefore, various means should be employed in the treatment of HS, such as ensuring airway ventilation, providing endotracheal intubation and mechanical ventilation, and conducting immediate hemostasis (Ogaki et al, 2014;Zhao et al, 2014). Adiponectin (ADPN) has a pronounced effect on vascular endothelial function; thus, much attention has and should be paid to the relationship between ADPN and HS (Lim et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Correlation between adiponectin and hemorrhagic shock in mice

Gong

Yao

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to explore the relationship between adiponectin (ADPN) and hemorrhagic shock (HS) and the recovery after HS. This is significant for further understanding of the pathophysiological processes of HS and the development of better treatments. In total, 72 male C57BL/6 mice were assigned randomly to three groups: control, HS, and recovery (N = 24). The HS mouse model was constructed by hemorrhage of the carotid artery and recovery was achieved by tail vein injection of Ringer's solution. The level of ADPN in the peripheral blood of mice before and after recovery was detected by enzyme-linked immunosorbent assay. Compared to control, HS mice showed significantly decreased ADPN levels with the extension of HS time while the level of ADPN in recovery mice increased significantly and remained high. The variation of ADPN levels was closely associated with the occurrence of HS in mice and their recovery, suggesting that ADPN might act as a biomarker of inflammation and have potential for the treatment of HS.

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Section: Introductionmentioning

confidence: 99%

Correlation between adiponectin and hemorrhagic shock in mice

Gong

Yao

et al. 2016

Genet. Mol. Res.

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“…In previous experimental I/R model, the observation of intestinal viability loss was usually limited to longterm ischemia (3 hours after injury) [36,37] . After ischemic injury, the study of monitoring intestinal permeability in vitro showed that it took at least 60 minutes to detect the change of intestinal barrier function, which could not quickly judge the change of intestinal viability [3] .…”

Section: Discussionmentioning

confidence: 99%

Photoacoustic Imaging in Evaluating Early Intestinal Ischemia and Reperfusion Injury with Rat Models

Wang¹,

Pan²,

Hua³

et al. 2020

Preprint

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Purpose: It is still a challenge to distinguish whether the damaged intestine is viable or not in the treatment of acute mesenteric ischemia. In this study, photoacoustic imaging (PAI) was used to observe the activity of intestinal tissue after ischemia and reperfusion injury in rats.Methods: With the approval of animal ethics committee our university, in vivo study was carried out. Forty male SD rats were randomly divided into four groups: sham operated (SO) group, 1 h ischemia group, 2 h ischemia group and ischemia-reperfusion (I/R) group. In the ischemia group, superior mesenteric artery (SMA) was isolated and clamped for 1 h (n = 10) and 2 h (n = 10), respectively. In I/R group, after ischemia for 1 hour, the clamp was removed and reperfused for 1 hour (n = 10). The same time interval of SMA was taken as SO group (n = 10). Immediately after the establishment of the animal model, PAI examination was performed. After PAI examination, the small intestine was collected for histopathology.Results: The PAI derived parameters of 2 h ischemia group were significantly different from those of SO group and I/R group (P < 0.05). The levels of Hb, HbR, map760 and map 840 were increased in different degrees in ischemia group, especially in 2 h ischemia group (compared with SO group, P < 0.01). With the prolongation of ischemia time, the injury was aggravated. In immunohistochemistry, compared with SO group, BAX increased significantly in 2 h ischemia group (P < 0.01). Similarly, Caspase-3 was significantly higher than the baseline level (P < 0.05). The level of HIF-1a increased in 2 h ischemia group and I/R group (P < 0.05). TUNEL staining showed that the number of apoptotic positive nuclei in 2 h ischemia group was significantly higher than that in SO group (P < 0.05). HE staining showed that ischemia for 2 hours was the most serious, with obvious mucosal damage, extensive epithelial injury and bleeding.Conclusions: PAI can be used as an effective tool to detect acute intestinal ischemia injury and quantitatively evaluate tissue viability.

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“…During hemorrhagic shock, increased levels of ICAM-1 induce s adhesion and activation of inflammatory cells, which is a key indicator of uncontrolled inflammatory responses and organ dysfunction (32). Interference with ICAM-1 may be beneficial for regaining and/or maintaining the balance of inflammation and organ function (32)(33)(34). In the current study, ICAM-1 was increased in plasma, and TNF-α was increased in plasma, lung, kidney, liver and myocardium samples following PHSML injection, suggesting that tissue injury was caused by ICAM-1-induced inflammation.…”

Section: Discussionmentioning

confidence: 99%

Intravenous injection of post‑hemorrhagic shock mesenteric lymph induces multiple organ injury in rats

et al. 2018

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Post-hemorrhagic shock mesenteric lymph (PHSML) has an important role in the multiple organ injuries caused by severe shock. The current study investigated whether intravenous injection of PHSML induces organ injury in normal rats. Following the establishment of hemorrhagic shock in donor rats (40±2 mmHg, 3 h), PHSML was drained during hypotension at 1-3 h and then injected to normal rats through the femoral vein within 30 min. The mean arterial pressure (MAP) was measured, and samples were obtained for analysis of histology and biochemical indices at 2.5 h post-PHSML administration. PHSML administration resulted in a significant decrease in MAP at the early and late stage of the experiment. Structural damage of the lung, kidney, heart and liver was also observed, and the levels of urea, creatinine, aspartate aminotransferase, total bile acid and creatine kinase MB isoenzyme were increased in the plasma. Additionally, PHSML injection significantly increased the levels of trypsin, tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 and receptor of advanced glycation end-products in the plasma, malondialdehyde in the lung and myocardium, and TNF-α in the lung, kidney, myocardium and liver. Intravenous injection of PHSML induced multiple organ injury in normal rats via increases in trypsin activity, inflammatory factors and free radical production. The findings indicate that PHSML return is an important contributor to organ damage following hemorrhagic shock.

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Utilization of Extracorporeal Membrane Oxygenation Alleviates Intestinal Ischemia–Reperfusion Injury in Prolonged Hemorrhagic Shock Animal Model

Cited by 18 publications

References 37 publications

Correlation between adiponectin and hemorrhagic shock in mice

Correlation between adiponectin and hemorrhagic shock in mice

Photoacoustic Imaging in Evaluating Early Intestinal Ischemia and Reperfusion Injury with Rat Models

Intravenous injection of post‑hemorrhagic shock mesenteric lymph induces multiple organ injury in rats

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