Utilization of experimental designs for optimization of Rhizopus arrhizus culture

Lhomme, Bibiane; Roux, J.-C.

doi:10.1016/0960-8524(91)90129-8

Cited by 16 publications

(7 citation statements)

References 10 publications

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“…These optimization methods involve three major steps: performing statistically designed experiments, estimating coefficients in a mathematical model to predict the response and checking the adequacy of the model [11,12]. RSM has been widely used in the optimization of the fermentation process such as media components for the production of enzyme [13,14], exopolysaccharides [15], other metabolites [16][17][18], biomass [19], and for predicting the growth rate of Bacillus cereus [20]. RSM is an effective tool for optimizing the process [21] when many factors and interactions affect the desired response.…”

Section: Introductionmentioning

confidence: 99%

Optimization of media components for enhanced arachidonic acid production by Mortierella alpina under submerged cultivation

Nisha

Rastogi

Venkateswaran

2011

Biotechnol Bioproc E

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Mortierella alpina, an oleaginous zygomycete is a potent producer of arachidonic acid, the pharmaceutically and nutraceutically important polyunsaturated fatty acid of the n-6 series. It serves a wide variety of purposes, from being a purely structural element in phospholipids to being involved in signal transduction, and as a substrate for a host of derivatives involved in second messenger function. Arachidonic acid has applications in diverse areas including infant and geriatric nutrition. In the present study, the interactive effects of four major media constituents on arachidonic acid production were investigated by applying a central composite rotatable design (CCRD) and response surface methodology (RSM). The independent variables, which were selected byconcentrations of glucose, corn solids, KH 2 PO 4 , and KNO 3 influenced the production of biomass, total lipid, and arachidonic acid by M. alpina. A second-order polynomial was fitted by multiple regression analysis of the experimental data. The optimum conditions (glucose 10.0 g/L, corn solids 5.0 g/L, KH 2 PO 4 1.0 g/L, and KNO 3 1.0 g/L) resulted in maximum production of arachidonic acid (1.39 g/L) and the corresponding biomass and total lipid concentrations were 12.49 and 5.87 g/L, respectively.

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Section: Introductionmentioning

confidence: 99%

Optimization of media components for enhanced arachidonic acid production by Mortierella alpina under submerged cultivation

Nisha

Rastogi

Venkateswaran

2011

Biotechnol Bioproc E

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“…All experiments were carried out with the optimal culture conditions determined in our previous work (Lhomme and Roux 1991): sucrose approx., 130 g. 1-1; yeast extract, 15 g.l-1; NH4NO3, 3.7 g.l-1; a 20/1 C/N ratio; 30°C, and a sufficient inoculum size of 1500-5000 spores, ml-1. No pH regulation was used.…”

Section: Methodsmentioning

confidence: 99%

“…Initial results (Lhomme and Roux 1991) showed that biomass production was 10.8 g.1-1 and 18.6 g.1-1 after 5-and 10-day cultures, respectively, in a medium containing 130 g.1-1 of sucrose, 15 g.1-1 of yeast extract and 3.7 g.1-1 of NHaNO3. The total organic carbon (TOC) decreased by about 52 and 80%, respectively.…”

Section: Introductionmentioning

confidence: 99%

Biomass production, carbon and oxygen consumption by Rhizopus arrhizus grown in submerged cultures on thin liquid films or immobilized on fibrous and particulate materials

Lhomme

Roux

1992

Appl Microbiol Biotechnol

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Oxygen consumption of Rhizopus arrhizus cultures was studied in order to understand why anaerobic-type metabolism takes place during growth, with high lactic acid synthesis. Rather than insufficient oxygen supply, pellet-type morphology was found to be responsible. Cultures with carriers and on thin liquid films were investigated with very good results for the second method. Not only was biomass production improved, but also carbon consumption was higher and lactic acid synthesis less compared with traditional submerged flask cultures.

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“…RSM is a powerful and efficient mathematical approach widely applied in the optimization of fermentation processes, e.g. medium components for enzyme production (Adinarayana and Ellaiah 2002;Bernal et al 2006;Anbu et al 2007;Cai and Zheng 2009;Sharma et al 2009), production of other metabolites (Zhang et al 1996) and biomass production (Lohomme and Roux 1991;Rucka et al 1998). The method can give information about the interaction between variables, provide information necessary for design and process optimization, reduce the number of experiments, and give multiple responses at the same time (Box and Behnken 1960;Chang et al 2002;Jain and Nian-fa 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimizing the biodegradation of two keratinous wastes through a Bacillus subtilis recombinant strain using a response surface methodology

2010

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Statistical optimization of the biodegradation of two keratinous wastes directed by Bacillus subtilis recombinant cells was carried out by means of a response surface methodology. A Box-Behnken design was employed to predict the optimal levels of three variables namely, keratin percent, incubation time and inoculum size. Analysis of variance revealed that, only keratin percent had the highest significant effect. Canonical analysis and ridge max analysis were used to get the optimal levels of the three predictors along with the optimum levels of the responses. The optimal sets of predicted and validated levels of the three variables were [7.69% (w/v) feathers, 96.58 h and 1.28% (v/v) inoculum size] and [8% (w/v) feathers, 98.45 h, 3.9% (v/v) inoculum size] to achieve the highest levels of soluble proteins (1.25-1.7 mg/ml) and NH(2)-free amino groups (245.82-270.0 μmol leucine/ml), respectively upon using three optimized feathers-based media. These values represented 83.67-100% and 100% adequacy for the models of soluble proteins and NH(2)-free amino groups, respectively. While, [8.23% (w/v) sheep wool, 5.52% (v/v) inoculum size and 46.58 h] and [8.33% (w/v) sheep wool, 5.89% (v/v) inoculum size and 63.46 h] were the optimal sets of predicted and validated levels of the above variables to achieve the highest yields of soluble proteins (3.4-4.6 mg/ml) and NH(2)-free amino groups (290.9-302.0 μmol leucine/ml), respectively upon using three optimized sheep wool-based media. These values represented 100% adequacy for the models of soluble proteins and NH(2)-free amino groups. By the end of the optimization strategy, a fold enhancement (2.14-2.43 and 1.78-2.12) in the levels of released soluble proteins and NH(2)-free amino groups, respectively was obtained upon using three optimized feathers-based media. However, a fold enhancement (4.25-5.75 and 2.42-2.5) in the levels of soluble proteins and NH(2)-free amino groups, respectively was obtained upon using three optimized sheep wool-based media. Data would encourage pilot scale optimization of the biodegradation of these wastes.

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Utilization of experimental designs for optimization of Rhizopus arrhizus culture

Cited by 16 publications

References 10 publications

Optimization of media components for enhanced arachidonic acid production by Mortierella alpina under submerged cultivation

Optimization of media components for enhanced arachidonic acid production by Mortierella alpina under submerged cultivation

Biomass production, carbon and oxygen consumption by Rhizopus arrhizus grown in submerged cultures on thin liquid films or immobilized on fibrous and particulate materials

Optimizing the biodegradation of two keratinous wastes through a Bacillus subtilis recombinant strain using a response surface methodology

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