Utilization of bioremediation processes for the treatment of PAH-contaminated sediments

Hughes, Joseph B.; Beckles, Denise M.; Chandra, Swati; Ward, C. H.

doi:10.1038/sj.jim.2900308

Cited by 106 publications

(49 citation statements)

References 2 publications

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“…The cost of aeration may also be prohibitive, and it may be more practical and economical to add nitrate, which is very water-soluble, as an electron acceptor. Bioagumentation with PAH-degrading bacteria and fungi has been tried with both successes and failures (4,23), and it is still not clear why inoculation sometimes fails.…”

mentioning

confidence: 99%

Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils

Eriksson

Södersten

et al. 2003

Appl Environ Microbiol

225

108

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The potential for biodegradation of polycyclic aromatic hydrocarbons (PAHs) at low temperature and under anaerobic conditions is not well understood, but such biodegradation would be very useful for remediation of polluted sites. Biodegradation of a mixture of 11 different PAHs with two to five aromatic rings, each at a concentration of 10 g/ml, was studied in enrichment cultures inoculated with samples of four northern soils. Under aerobic conditions, low temperature severely limited PAH biodegradation. After 90 days, aerobic cultures at 20°C removed 52 to 88% of the PAHs. The most extensive PAH degradation under aerobic conditions at 7°C, 53% removal, occurred in a culture from creosote-contaminated soil. Low temperature did not substantially limit PAH biodegradation under nitrate-reducing conditions. Under nitrate-reducing conditions, naphthalene, 2-methylnaphthalene, fluorene, and phenanthrene were degraded. The most extensive PAH degradation under nitrate-reducing conditions at 7°C, 39% removal, occurred in a culture from fuel-contaminated Arctic soil. In separate transfer cultures from the above Arctic soil, incubated anaerobically at 7°C, removal of 2-methylnaphthalene and fluorene was stoichiometrically coupled to nitrate removal. Ribosomal intergenic spacer analysis suggested that enrichment resulted in a few predominant bacterial populations, including members of the genera Acidovorax, Bordetella, Pseudomonas, Sphingomonas, and Variovorax. Predominant populations from different soils often included phylotypes with nearly identical partial 16S rRNA gene sequences (i.e., same genus) but never included phylotypes with identical ribosomal intergenic spacers (i.e., different species or subspecies). The composition of the enriched communities appeared to be more affected by presence of oxygen, than by temperature or source of the inoculum.

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mentioning

confidence: 99%

Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils

Eriksson

Södersten

et al. 2003

Appl Environ Microbiol

225

108

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“…Bioremediation is a cost-effective process to degrade wastes, and techniques are reviewed elsewhere (23)(24)(25)(26). Bioremediation takes advantage of the ability of bacteria (27)(28)(29)(30), fungi (31,32), and plants (33) to degrade many toxicants.…”

mentioning

confidence: 99%

Genotoxicity of bioremediated soils from the Reilly Tar site, St. Louis Park, Minnesota.

Hughes

Claxton

Brooks

et al. 1998

Environ Health Perspect

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An in vitro approach was used to measure the genotoxicity of creosote-contaminated soil before and after four bioremediation processes. water-soluble mutagens accounted for 40% of the total mutagenic activity and they were stable at room temperature. The mutagenic activity in the bioslurry and biopile samples was due to either the processes themselves or to the added sludge/manure amendments. The in vitro approach was effective in monitoring bioremediated soils for genotoxicity and will be useful in future laboratory and in situ studies.

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“…PAHs continuously increase and accumulate in the soil and water, due to the human activities that cause the emissions of these contaminants into the environment every day, and thus appropriate treatment is required to reduce the concentration and toxicity of these . There has been an increased interest in developing and understanding the microbial degradation processes when PAHs are present in complex mixtures, because a mixture of contaminants in a bioremediation system may result in inhibition, co-metabolism or augmentation (Hughes et al, 1997). For example, both inhibition and co-metabolism have been observed in the degradation of a mixture of phenanthrene and fluorantene by a Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Certain Polycyclic Hydrocarbons with Paenbacillus alvei and Penicillum restricum

Ogunbayo¹,

Olanipekun²,

Owoade³

2018

J. Ecol. Eng.

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The biodegradation of polycyclic aromatic hydrocarbons (PAHs) such as naphthalene, 2-methylnaphthalene and anthracene was investigated using pure and mixed culture of Paenbacillus alvei (bacteria) and Penicillum restricum (fungi) (isolated from crude oil contaminated sites in Rivers state of Nigeria). The abilities of these organisms to biodegrade the PAHs were studied by growing the isolates in a mineral salt medium (MSM) with the PAHs in shake flasks placed in a shaking water bath rotating at 150 rpm at room temperature for 21 days. The samples were withdrawn every three days for analysis of the residual PAHs using SRI 8610C Gas Chromatograph (GC), while the growth of the organisms was determined by using the dry biomass method. The results showed that the concentrations of PAHs decreased with an increase in the exposure time throughout a 21-day period, thus confirming the abilities of the organisms to feed on the PAHs. The results showed that the bacteria had more affinity for naphthalene, while the fungi had more affinity for anthracene. It was, however, observed that the samples from the flask which contained mixed PAHs and mixed culture of Paenbacillus alvei and Penicillum restricum had the highest and most significant biomass growth thus suggesting a synergy between the two organisms.

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Utilization of bioremediation processes for the treatment of PAH-contaminated sediments

Cited by 106 publications

References 2 publications

Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils

Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils

Genotoxicity of bioremediated soils from the Reilly Tar site, St. Louis Park, Minnesota.

Biodegradation of Certain Polycyclic Hydrocarbons with Paenbacillus alvei and Penicillum restricum

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