Utilization of biodiesel by-product as substrate for high-production of β-farnesene via relatively balanced mevalonate pathway in Escherichia coli

You, Shengping; Yin, Qingdian; Zhang, Jianye; Zhang, Chengyu; Qi, Wei; Gao, Lan; Zhang, Tao; Su, Rongxin; He, Zhimin

doi:10.1016/j.biortech.2017.06.058

Cited by 54 publications

(38 citation statements)

References 35 publications

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“…In S. cerevisiae , > 40 g L −1 of amorphadiene (Westfall et al 2012) and 25 g L −1 of artemisinic acid (Paddon et al 2013) that can be chemically converted to artemisinin were produced. In comparison with these values, the maximum titers reported for E. coli are 8.74 g L −1 of β-farnesene (You et al 2017) and 27.4 g L −1 of amorphadiene (Tsuruta et al 2009). Similarly, for two more sesquiterpenoids that can be produced in the low g L −1 range, S. cerevisiae appears to be superior to E. coli at the moment.…”

Section: Microbial Production Of Different Terpenoid Classesmentioning

confidence: 96%

Identifying and engineering the ideal microbial terpenoid production host

Moser

Pichler

2019

Appl Microbiol Biotechnol

114

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More than 70,000 different terpenoid structures are known so far; many of them offer highly interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of these compounds from their natural sources or chemical synthesis is-in many cases-technically challenging with low or moderate yields while wasting valuable resources. Microbial production of terpenoids offers a sustainable and environment-friendly alternative starting from simple carbon sources and, frequently, safeguards high product specificity. Here, we provide an overview on employing recombinant bacteria and yeasts for heterologous de novo production of terpenoids. Currently, Escherichia coli and Saccharomyces cerevisiae are the two best-established production hosts for terpenoids. An increasing number of studies have been successful in engineering alternative microorganisms for terpenoid biosynthesis, which we intend to highlight in this review. Moreover, we discuss the specific engineering challenges as well as recent advances for microbial production of different classes of terpenoids. Rationalizing the current stages of development for different terpenoid production hosts as well as future prospects shall provide a valuable decision basis for the selection and engineering of the cell factory(ies) for industrial production of terpenoid target molecules.

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Section: Microbial Production Of Different Terpenoid Classesmentioning

confidence: 96%

Identifying and engineering the ideal microbial terpenoid production host

Moser

Pichler

2019

Appl Microbiol Biotechnol

114

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“…You et al constructed a β-farnesene producing strain of E. coli and overexpressed IDI and farnesyl diphosphate synthase (FPPS) to minimize isopentenyl diphosphate (IPP) accumulation. The titer of the final strain reached 8.74 g/L using glycerol as carbon source [28]. In S. cerevisiae , Meadows et al rewired central carbon metabolism with four non-native metabolic reactions, enabling biosynthesis of cytosolic acetyl-CoA with a reduced ATP requirement, reduced loss of carbon to CO 2 -emitting reactions, and improved pathway redox balance.…”

Section: Introductionmentioning

confidence: 99%

Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene

Liu

Xin

Cui

et al. 2019

Biotechnol Biofuels

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BackgroundYarrowia lipolytica, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineered Y. lipolytica is still low. α-Farnesene, a sesquiterpene, can be used in medicine, bioenergy and other fields, and has very high economic value. Here, we used α-farnesene as an example to explore the potential of Y. lipolytica for terpenoid production.ResultsWe constructed libraries of strains overexpressing mevalonate pathway and α-farnesene synthase genes by non-homologous end-joining (NHEJ) mediated integration into the Y. lipolytica chromosome. First, a mevalonate overproduction strain was selected by overexpressing relevant genes and changing the cofactor specificity. Based on this strain, the downstream α-farnesene synthesis pathway was overexpressed by iterative integration. Culture conditions were also optimized. A strain that produced 25.55 g/L α-farnesene was obtained. This is the highest terpenoid titer reported in Y. lipolytica.ConclusionsYarrowia lipolytica is a potentially valuable species for terpenoid production, and NHEJ-mediated modular integration is effective for expression library construction and screening of high-producer strains.

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“…Escherichia coli has been successfully engineered to produce a number of isoprenoid jet fuel precursors (Peralta-Yahya et al, 2011;Mendez-Perez et al, 2017;You et al, 2017). Although this species is capable of synthesizing the critical isoprenoid building blocks, IPP and DMAPP, via its native MEP pathway (Figure 1) yields were typically insufficient.…”

Section: E Colimentioning

confidence: 99%

“…As a major waste product of biodiesel production, crude glycerol is a promising low-cost, second generation feedstock. In a recent study by You et al (2017), the precursor to the approved jet fuel, farnesane, was produced from glycerol by an engineered E. coli strain. Although, encouraging titers of 8.7 g/L were achieved with pure glycerol, lower titers were achieved in subsequent experiments using crude glycerol.…”

Section: Glycerolmentioning

confidence: 99%

“…Although, encouraging titers of 8.7 g/L were achieved with pure glycerol, lower titers were achieved in subsequent experiments using crude glycerol. This was attributed to the presence of inhibitory compounds, indicating pre-treatment may be required to maximize productivity on crude glycerol (You et al, 2017). Glycerol was also shown to be preferred carbon source for limonene production by an engineered E. coli strain recently (Rolf et al, 2020).…”

Section: Glycerolmentioning

confidence: 99%

See 1 more Smart Citation

Sustainable Production of Microbial Isoprenoid Derived Advanced Biojet Fuels Using Different Generation Feedstocks: A Review

Walls

Rios‐Solis

2020

Front. Bioeng. Biotechnol.

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As the fastest mode of transport, the aircraft is a major driver for globalization and economic growth. The development of alternative advanced liquid fuels is critical to sustainable development within the sector. Such fuels should be compatible with existing infrastructure and derived from second generation feedstocks to avoid competition with food markets. With properties similar to petroleum based fuels, isoprenoid derived compounds such as limonene, bisabolane, farnesane, and pinene dimers are of increasing interest as "drop-in" replacement jet fuels. In this review potential isoprenoid derived jet fuels and progress toward their microbial production was discussed in detail. Although substantial advancements have been achieved, the use of first generation feedstocks remains ubiquitous. Lignocellulosic biomass is the most abundant raw material available for biofuel production, however, technological constraints associated with its pretreatment and saccharification hinder its economic feasibility for low-value commodity production. Non-conventional microbes with novel characteristics including cellulolytic bacteria and fungi capable of highly efficient lignocellulose degradation and xylose fermenting oleaginous yeast with enhanced ligninassociated inhibitor tolerance were investigated as alternatives to traditional model hosts. Finally, innovative bioprocessing methods including consolidated bioprocessing and sequential bioreactor approaches, with potential to capitalize on such unique natural capabilities were considered.

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Utilization of biodiesel by-product as substrate for high-production of β-farnesene via relatively balanced mevalonate pathway in Escherichia coli

Cited by 54 publications

References 35 publications

Identifying and engineering the ideal microbial terpenoid production host

Identifying and engineering the ideal microbial terpenoid production host

Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene

Sustainable Production of Microbial Isoprenoid Derived Advanced Biojet Fuels Using Different Generation Feedstocks: A Review

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