Utilization of betaine aldehyde by choline (chol-) mutants of Neurospora crassa

Richardson, Michael; Speed, D. J.

doi:10.1007/bf00410225

Cited by 3 publications

(2 citation statements)

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“…Growth can also be supported by the addition of betaine aldehyde but not betaine. As betaine aldehyde is an intermediate between choline and betaine, it appears that the oxidation of betaine aldehyde to betaine is irreversible (Richardson & Speed, 1969). If this is also true in F. graminearum, then betaine may act as a common intermediate in eliciting the morphological effects of choline and related analogues.…”

Section: Discussionmentioning

confidence: 99%

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Choline- and acetylcholine-induced changes in the morphology of Fusarium graminearum: evidence for the involvement of the choline transport system and acetylcholinesterase

et al. 1995

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The response of iusarium gramineanrm to choline, acetylcholine and a number of related analogues was investigated and their ability to induce a morphological response quantified. A number of mutants resistant to the alkylating agent nitrogen mustard (nim strains) were generated and found to have lost the ability to transport choline. These mutants were found to be insensitive to choline and acetylcholine but not to betaine, ethanolamine and other analogues. In addition, the nonlcompetitive inhibitor hemicholinium-3 was also found to reduce the morphological effect of choline, proving that transport of choline into the hypha is essential for the morphological response. Acetylcholinesterase inhibitors blocked the morphological response to acetylcholine but had no effect on the response to choline, suggesting the presence of a membrane-or wall-bound acetylcholinesterase that hydrolyses acetylcholine to choline which subsequently induces the morphological response. Studies on the in who chitin synthase activity revealed that addition of choline caused a transient 75% increase in chitin synthase activity within 30 s, the rate rapidly returning to that observed before the addition of choline.No such effect was observed with the nim mutants.

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Section: Discussionmentioning

confidence: 99%

“…&Speed, 1969). If this is also true in F. graminearum, then betaine may act as a common intermediate in eliciting the morphological effects of choline and related analogues.…”

mentioning

confidence: 97%