Utilization of Alcohols by Hansenula miso

Abstract

The ability of Hansenula miso IFO 0146 to utilize various alcohols and acidic salts as sole sources of carbon and the ability of resting cells to oxidize various alcohols and glucose were studied. Growing cells could utilize only ethanol, glycerol, acetate and lactate, while resting cells grown on ethanol medium could oxidize various alcohols such as 1,2-ethanediol, DL-1,2-propanediol, 1,3-propanediol, meso-2,3-butanediol, DL-1,3-butane diol, and 1,4-butanediol. From 2 g of 1,2-ethanediol and DL-1,3-butanediol, 1.3g of glycolic acid and 0.5 g of jS-hydroxybutyric acid respectively were produced. The organism formed D-arabinitol from glycerol and glucose, respectively. From 100 ml of culture in medium containing 6 ml of ethanol and 3.0 g of (NH4)2HPO4 as carbon and nitrogen sources 3.40g of dried cells were obtained.

Degradation of 1,4-Dioxane and Cyclic Ethers by an Isolated Fungus

“…There are several papers describing the degradation pathway for ethylene glycol by humans, yeasts, and bacteria (4,6,7,11,24). However, to our knowledge, no pathway has been proposed for filamentous fungi.…”

Growth of Acinetobacter calcoaceticus on Ethanol

“…There are two mechanisms for the formation of acetyl CoA from acetate. One occurs in mammalian tissue, yeast, and some bacteria and requires one enzyme, acetyl CoA synthetase (4). kinase and synthetase enzymes, and sulfur is an integral component of CoA.…”

Production of 3-hydroxypropionic acid by recombinant Klebsiella pneumoniae based on aeration and ORP controlled strategy

“…For example, Hansenula miso could transform 1,3-propandiol to 3-HP [5], Fusarium merismoides could transform 1,4-butanediol to 3-HP [6], and Candidar ugosa could transform propionic acid to 3-HP [7]. But for more large-scale biological production of 3-HP, it is desirable to use cheaper and more abundant technical substrates such as glycerol and glucose.…”