Utilization of agro-industrial waste for production of Transglutaminase from Streptomyces mobaraensis

Fatima, Syeda Warisul; Tiwari, Rameshwar; Khare, Sunil Kumar

doi:10.1016/j.biortech.2019.121391

Cited by 11 publications

(3 citation statements)

References 22 publications

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“…During fermentation, TGase activation can be enhanced by eliminating the zymogen region with proteases or by facilitating secretion with the inclusion of cetyltrimethylammonium bromide (CTAB) (Fatima et al, 2019). Furthermore, it has been discovered that Streptomyces strains with increased production efficiency and safety can be obtained through random mutagenesis and targeted genetic modification, making them potential candidates for industrial TGase production (Yin et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

LexA, an SOS response repressor, activates TGase synthesis in Streptomyces mobaraensis

Shi,

Yan,

Yuan

et al. 2024

Front. Microbiol.

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Transglutaminase (EC 2.3.2.13, TGase), an enzyme that catalyzes the formation of covalent cross-links between protein or peptide molecules, plays a critical role in commercial food processing, medicine, and textiles. TGase from Streptomyces is the sole commercial enzyme preparation for cross-linking proteins. In this study, we revealed that the SOS response repressor protein LexA in Streptomyces mobaraensis not only triggers morphological development but also enhances TGase synthesis. The absence of lexA significantly diminished TGase production and sporulation. Although LexA does not bind directly to the promoter region of the TGase gene, it indirectly stimulates transcription of the tga gene, which encodes TGase. Furthermore, LexA directly enhances the expression of genes associated with protein synthesis and transcription factors, thus favorably influencing TGase synthesis at both the transcriptional and posttranscriptional levels. Moreover, LexA activates four crucial genes involved in morphological differentiation, promoting spore maturation. Overall, our findings suggest that LexA plays a dual role as a master regulator of the SOS response and a significant contributor to TGase regulation and certain aspects of secondary metabolism, offering insights into the cellular functions of LexA and facilitating the strategic engineering of TGase overproducers.

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Section: Introductionmentioning

confidence: 99%

LexA, an SOS response repressor, activates TGase synthesis in Streptomyces mobaraensis

Shi,

Yan,

Yuan

et al. 2024

Front. Microbiol.

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“…Transcription analysis indicated that protease, CTAB, and MgCl 2 were proven to promote TGase expression in S. mobaraensis ( Fatima and Khare, 2021 ). To improve the economic efficiency, TGase fermentation was conducted using agricultural wastes, such as wheat bran and non-commercial potatoes ( Guerra-Rodriguez and Vazquez, 2014 ; Fatima et al, 2019 ). Compared to genetic modification, these “non-genetic” strategies are much easier to be accepted for food industries.…”

Section: Introductionmentioning

confidence: 99%

Improved Productivity of Streptomyces mobaraensis Transglutaminase by Regulating Zymogen Activation

Yin

Rao

Zhou

et al. 2022

Front. Bioeng. Biotechnol.

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Streptomyces mobaraensis transglutaminase (TGase) is extracellularly expressed as a zymogen and then activated by TGase-activating protease (TAP). In this study, we reported the strategy for improving TGase production via the regulation of TAP activity in S. mobaraensis. First, we analyzed the effects of three inorganic nitrogen sources on TGase production. With 30 mM nitrogen content, the time to the peak of TGase activity induced by (NH4)2SO4 or NH4Cl was 72 h, 12 h earlier than that of the fermentation without adding NH4+. SDS-PAGE analysis indicated that NH4+ accelerated the TGase activation in S. mobaraensis. Then, we examined the effect of NH4+ on TAP biosynthesis using a TGase-deficient S. mobaraensis strain. It showed that NH4+ enhanced the TAP activity at the early stage of the fermentation, which was dependent on the concentration and time of NH4+ addition. Last, the yield and productivity of S. mobaraensis TGase were increased by 1.18-fold and 2.1-fold, respectively, when optimal NH4+ addition (60 mM and 12 h) was used. The fermentation period was shortened from 84 to 48 h. The NH4+ addition also increased the storage stability of crude enzyme at room temperature. These findings will benefit the TGase production and its activation mechanism in S. mobaraensis.

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“…The accumulation of agro‐industrial waste has been growing every year recently. Government and other agencies are working together to reduce this trend 1 . In China, the increasing waste resulting from rapid industrial development and a huge population has also become a major problem.…”

Section: Introductionmentioning

confidence: 99%

Production of extracellular enzymes by a termite‐nest‐related Bacillus siamensisYC‐9 in solid‐state fermentation on agricultural by‐products

Wang

Zhou

Yin

et al. 2021

Biofuels Bioprod Bioref

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A Bacillus siamensis YC‐9 strain was isolated from termite nest. The strain was used to produce several important industrial enzymes by utilizing different agricultural by‐products with solid‐state fermentation (SSF). It was observed that most of the substrates that were studied were strongly positively affected by alkaline treatment for the production of enzymes. The highest yields for protease, α‐amylase, and cellulase were 82.87, 42.81, and 1.54 IU g−1, achieved on alkaline‐treated corn pericarp, rice bran, and wheat bran respectively. However, untreated soybean meal was the best choice for the secretion of pectinases (66.65 IU g−1). In general, ultrasonic pretreatment was more effective than microwave treatment and it promoted the production of proteases on soybean meal, sunflower meal, and wheat bran. The findings of the present study will provide some theoretical basis for enzyme production in the future. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd.

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Utilization of agro-industrial waste for production of Transglutaminase from Streptomyces mobaraensis

Cited by 11 publications

References 22 publications

LexA, an SOS response repressor, activates TGase synthesis in Streptomyces mobaraensis

LexA, an SOS response repressor, activates TGase synthesis in Streptomyces mobaraensis

Improved Productivity of Streptomyces mobaraensis Transglutaminase by Regulating Zymogen Activation

Production of extracellular enzymes by a termite‐nest‐related Bacillus siamensisYC‐9 in solid‐state fermentation on agricultural by‐products

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