Utilization of agro-industrial waste for biosurfactant production under submerged fermentation and its application in oil recovery from sand matrix

Das, Amar Jyoti; Kumar, Rajesh

doi:10.1016/j.biortech.2018.03.093

Cited by 103 publications

(44 citation statements)

References 44 publications

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“…Due to such unique functional properties and eco-friendly nature, these biological surfactants are expected to become multi-functional constituents of the twenty-first century. Additionally, they have more applications in industrial sectors such pharmaceutical, textile processing, agricultural, cosmetics, personal care, food industries, and environmental applications like soil remediation, hydrocarbon degradation and oil recovery [ 4 , 9 – 11 ]. Biosurfactant can be produced by numerous bacterial species.…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Phulpoto

et al. 2020

Microb Cell Fact

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Background: Biosurfactants, being highly biodegradable, ecofriendly and multifunctional compounds have wide applications in various industrial sectors including environmental bioremediation. Surfactin, a member of lipopeptide family, which is considered as one of the most powerful biosurfactants due to its excellent emulsifying activities as well as environmental and therapeutic applications. Therefore, the aim of this study was to investigate the newly isolated bacterial strain S2MT for production of surfactin-like biosurfactants and their potential applications for oilcontaminated soil remediation. Results: In this study, the strain S2MT was isolated from lake sediment and was identified as Bacillus nealsonii based on transmitted electron microscopy (TEM) and 16S rRNA ribo-typing. The strain S2MT produced biosurfactant that reduced the surface tension (34.15 ± 0.6 mN/m) and displayed excellent emulsifying potential for kerosene (55 ± 0.3%). Additionally, the maximum biosurfactant product yield of 1300 mg/L was achieved when the composition of the culture medium was optimized through response surface methodology (RSM). Results showed that 2% glycerol and 0.1% NH 4 NO 3 were the best carbon/nitrogen substrates for biosurfactant production. The parameters such as temperature (30 °C), pH (8), agitation (100 rpm), NH 4 NO 3 (0.1%) and NaCl (0.5%) displayed most significant contribution towards surface tension reduction that resulted in enhanced biosurfactant yield. Moreover, the extracted biosurfactants were found to be highly stable at environmental factors such as salinity, pH and temperature variations. The biosurfactants were characterized as cyclic lipopeptides relating to surfactin-like isoforms (C 13-C 15) using thinlayer chromatography (TLC), Ultra high performance liquid chromatography and mass spectrometry (UHPLC-MS). The crude biosurfactant product displayed up to 43.6 ± 0.08% and 46.7 ± 0.01% remediation of heavy engine-oil contaminated soil at 10 and 40 mg/L concentrations, respectively. Conclusion: Present study expands the paradigm of surfactin-like biosurfactants produced by novel isolate Bacillus nealsonii S2MT for achieving efficient and environmentally acceptable soil remediation as compared to synthetic surfactants.

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Section: Introductionmentioning

confidence: 99%

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Phulpoto

et al. 2020

Microb Cell Fact

View full text Add to dashboard Cite

show abstract

“…Due to such unique functional properties and eco-friendly nature, these biological surfactants are expected to become multi-functional constituents of the 21st century. Additionally, they have more applications in industry sectors such pharmaceutical, textile processing, agricultural, cosmetics, personal care, food industries, and environmental applications like soil remediation, hydrocarbon degradation and oil recovery [4,[9][10][11]. Biosurfactant can be produced by numerous bacterial species.…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Phulpoto

et al. 2020

Preprint

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Background Biosurfactants, being highly biodegradable, ecofriendly and multifunctional compounds have wide applications in various industrial sectors including environmental bioremediation. Surfactin a member of lipopeptide family which considered as one of the most powerful biosurfactant due to its environmental applications, emulsification activities as well as therapeutic properties. Therefore, the aim of this study to investigate the surfactin like biosurfactants produced by newly strain S2MT and their potential applications for soil remediation. Results In this study, a novel biosurfactant producing strain Bacillus nealsonii S2MT was investigated from the lake sediment that reduced the surface tension 34.15± 0.6 mN/m -1 with excellent emulsifying potential 55± 0.3% in kerosene oil. Additionally, the highest biosurfactant product 1300 mg/L -1 was achieved during which the composition of the culture medium was optimized through a response surface methodology (RSM). Results showed that 2% glycerol and 0.1% NH 4 NO 3 were the best carbon/nitrogen substrates for biosurfactant production. The most significant parameters such as temperature (30 °C), pH (8), agitation (100 rpm), NH 4 NO 3 (0.1%), yeast extract (0%) and NaCl (0.5%) contributed to the surface tension reduction and therefore enhancing the biosurfactant yield. Moreover, the obtained product was found to be highly stable at environmental factors such as salinity, pH and temperature variations. In addition, the biosurfactant product was chemically characterized as cyclic lipopeptide relating to surfactin-like isoforms (C 13 -C 15 ) with a thin-layer chromatography (TLC) and liquid chromatography and mass spectroscopy (LC/MS) respectively. The corresponding biosurfactant displayed 43.6± 0.08 and 46.7± 0.01% remediation of heavy engine-oil contaminated soil at 10 and 40 mg/L concentrations respectively. Conclusion This study, therefore, confirmed that the strain S2MT was not only a potential biosurfactant producer but also an efficient and environmentally acceptable candidate for soil remediation compared to synthetic compounds.

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“…The activity of the BS produced by NPK was similar to those reported by (Lan et al . 2015; Das and Kumar 2018; Lee et al . 2018).…”

Section: Resultsmentioning

confidence: 99%

Enhanced biodegradation of crude oil in soil by a developed bacterial consortium and indigenous plant growth promoting bacteria

et al. 2020

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Aims This study aimed to develop an efficient, cost‐effective and eco‐friendly bacterial consortium to degrade petroleum sludge. Methods and Results Four bacterial strains belonging to genera Acinetobacter and Pseudomonas were selected to constitute three different consortia based on their initial concentration. The highest degradation rate (78%) of 1% (v/v) crude oil after 4 weeks of incubation was recorded when the concentration of biosurfactant (BS) producing isolate was high. Genes, such as alkB, almA, cyp153, pah‐rhdGN, nah, phnAC and cat23 were detected using the polymerase chain reaction method and their induction levels were optimal at pH 7·0. A crude oil sludge was artificially constituted, and its bacterial composition was investigated using 16S rRNA gene amplicon sequencing. The results showed that the soil bacterial community was dominated by plant growth‐promoting bacteria (PGPB) after crude oil treatment. Conclusions Our findings indicate the decontamination of the crude oil contaminated soil was more effective in the presence of both the constituted consortium and PGPB compared to the presence of PGPB alone. Significance and Impact of the Study This study showed that the PGPB (Taibaiella) present in petroleum uncontaminated soil can promote the soil decontamination. The addition of both efficient hydrocarbon‐degrading and BS producing bacteria is also necessary to improve the decontamination.

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Utilization of agro-industrial waste for biosurfactant production under submerged fermentation and its application in oil recovery from sand matrix

Cited by 103 publications

References 44 publications

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation

Enhanced biodegradation of crude oil in soil by a developed bacterial consortium and indigenous plant growth promoting bacteria

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