Utilization of Agro-Industrial Byproducts for Bacteriocin Production Using Enterococcus spp. Strains Isolated from Patagonian Marine Invertebrates

Sosa, Franco; Parada, Romina B.; Marguet, Emilio Rogelio; Vallejo, Marisol

doi:10.1007/s00284-021-02712-5

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…Antibacterial activity is measured in arbitrary units per milliliter (AU/ml). One arbitrary unit is defined as the reciprocal of the highest dilution that exhibits a minimum detectable zone of inhibition [25] . The activity, yield, and fold purification of bacteriocin obtained after various purification steps were also calculated.…”

Section: Methodsmentioning

confidence: 99%

Characterization of novel bacteriocin PB2 and comprehensive detection of the pediocin gene ped-A1 from Pediococcus pentosaceus PB2 strain isolated from a sorghum-based fermented beverage in Nigeria

Otunba¹,

Osuntoki

Okunowo

et al. 2022

Biotechnology Reports

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Section: Methodsmentioning

confidence: 99%

Characterization of novel bacteriocin PB2 and comprehensive detection of the pediocin gene ped-A1 from Pediococcus pentosaceus PB2 strain isolated from a sorghum-based fermented beverage in Nigeria

Otunba¹,

Osuntoki

Okunowo

et al. 2022

Biotechnology Reports

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“…According to the literature, one of the mechanisms of the antagonistic action of enterococci is their ability to produce bacteriocins, which allows the use of bacteriocinogenic Enterococcus strains as protective starter cultures, as well as producers of bacteriocins that can be used as purified preparations (Ng et al, 2020). Bacteriocins of lactic acid bacteria (LAB) have been intensively studied over the past decades and there is still more interest in screening for new producer strains (Nami et al, 2019;Lauková et al, 2020;Choeisoongnern et al, 2021;Sosa et al, 2021). According to a definition, bacteriocins are ribosomally synthesized peptides that inhibit the growth of phylogenetically closely related microorganisms (Darbandi et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Most studies on the production of enterocins have been conducted using commercial laboratory media such as MRS, BHI, and M17. Commonly, after the initial screening of bacteriocinogenic LAB strains, the next stage is the optimization of culture conditions for large-scale biomass or bacteriocin production using cheaper cultural media (Metsoviti et al, 2011;Schirru et al, 2014;Sosa et al, 2021). Data concerning enterocin production by Enterococcus strains using inexpensive media are very rare and mainly concern certain strains (Coetzee et al, 2007;López et al, 2007;Bali et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Screening of bacteriocin-producing dairy Enterococcus strains using low-cost culture media

Garmasheva

Oleschenko

2023

Front. Microbiol.

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This study was carried out to select the bacteriocinogenic strains among Enterococcus strains isolated from Ukrainian traditional dairy products using a low-cost media for screening, that containing molasses and steep corn liquor. A total of 475 Enterococcus spp. strains were screened for antagonistic activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes indicator strains. The initial screening revealed that 34 Enterococcus strains during growth in low-cost medium containing corn steep liquor, peptone, yeast extract, and sucrose produced metabolites with inhibition activity against at least of the indicator strains used. Enterocin genes entA, entP, and entB were detected in 5 Enterococcus strains by PCR assay. Genes of enterocins A and P were found in E. faecalis 58 and Enterococcus sp. 226 strains, enterocins B and P – in Enterococcus sp. 423, enterocin A – in E. faecalis 888 and E. durans 248 strains. Bacteriocin-like inhibitory substances (BLIS) produced by these Enterococcus strains were thermostable and sensitive to proteolytic enzymes. To our knowledge, this is the first report on the isolation of enterocin-producing wild Enterococcus strains from traditional Ukrainian dairy products using a low-cost media for screening bacteriocinogenic strains. Strains E. faecalis 58, Enterococcus sp. 423, and Enterococcus sp. 226 are promising candidates for practical use as producers of bacteriocins with inhibitory activity against L. monocytogenes using molasses and steep corn liquor as cheap sources of carbon and nitrogen, that can significantly reduce the cost of industrial bacteriocin production. Further studies will be required to determine the dynamic of bacteriocin production, its structure, and mechanisms of antibacterial action.

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“…El litoral marino de la Argentina constituye una fuente de BAL, provenientes de distintos vertebrados e invertebrados, que exhiben propiedades biotecnológicas aplicables en la industria alimentaria, tales como actividad antimicrobiana (Marguet et al 2011;Sosa et al 2022), producción de bioemulsificantes (Gianni de Carvalho et al 2019;Lara et al 2020), características probióticas (Sequeiros et al 2010), entre otras. A lo largo de los últimos años, se ha profundizado el estudio de la capacidad antioxidante de BAL provenientes de organismos terrestres y alimentos fermentados o sin procesar; no obstante, es escasa la información sobre cepas de origen marino.…”

Section: Introductionunclassified

Capacidad antioxidante de bacterias lácticas aisladas de peces e invertebrados marinos de la provincia de Chubut, Patagonia-Argentina

Sosa,

Parada,

Sánchez-Cabrera

et al. 2023

Rev. Cien. Mar. Cost.

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La actividad antioxidante de las bacterias ácido lácticas (BAL) ha sido estudiada en profundidad en cepas aisladas de organismos terrestres, alimentos fermentados y sin procesar. Sin embargo, esta información en aislamientos de origen marino aún está siendo determinada. Tal característica presenta un interés particular, debido a su uso potencial en alimentos funcionales. En este trabajo, se estudió la capacidad antioxidante de 16 BAL bacteriocinogénicas provenientes de organismos marinos de la costa del Chubut (periodo 2010-2022), mediante ensayos in vitro e in vivo. Se evaluó la capacidad antioxidante in vitro por medio de la reducción de 2,2-difenil-1-picrihidrazilo (DPPH), actividad antioxidante reductora de iones cúpricos (CUPRAC) y barrido de radicales oxidrilos, empleando células sin lisar. Se utilizó Saccharomyces cerevisiae como modelo biológico eucariota para valorar el efecto protector in vivo. Los resultados obtenidos por secuestro de radicales DPPH exhibieron valores entre 15-37.5%; el ensayo de secuestro de radicales oxidrilos mostró valores entre 13.3-61.9% y el método CUPRAC los valores variaron entre 0.223-0.551, equivalentes a mmol de ácido ascórbico por ml de suspensión celular. El efecto protector de las BAL en la supervivencia de S. cerevisiae presentó valores entre 36-90%. No fue posible establecer relaciones entre los valores obtenidos por distintos métodos o entre miembros del mismo género o especie; por ello, la capacidad antioxidante podría considerarse una característica dependiente de la cepa. Los resultados sugieren que la capacidad antioxidante de BAL bacteriocinogénicas de origen marino podría usarse como un carácter metabólico de selección con potencial biotecnológico en el desarrollo de alimentos funcionales.

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Utilization of Agro-Industrial Byproducts for Bacteriocin Production Using Enterococcus spp. Strains Isolated from Patagonian Marine Invertebrates

Cited by 5 publications

References 42 publications

Characterization of novel bacteriocin PB2 and comprehensive detection of the pediocin gene ped-A1 from Pediococcus pentosaceus PB2 strain isolated from a sorghum-based fermented beverage in Nigeria

Characterization of novel bacteriocin PB2 and comprehensive detection of the pediocin gene ped-A1 from Pediococcus pentosaceus PB2 strain isolated from a sorghum-based fermented beverage in Nigeria

Screening of bacteriocin-producing dairy Enterococcus strains using low-cost culture media

Capacidad antioxidante de bacterias lácticas aisladas de peces e invertebrados marinos de la provincia de Chubut, Patagonia-Argentina

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