Utilization of a floral organ-expressing AP1 promoter for generation of new floral traits in Torenia fournieri Lind

Sasaki, Keiko; Yamaguchi, Hiroyasu; Narumi, Takayuki; Shikat, Masahito; Oshima, Yoshimi; Nakata, Masaru; Mitsuda, Nobutaka; Ohme‐Takagi, Masaru; Ohtsubo, Norihiro

doi:10.5511/plantbiotechnology.11.0124b

Cited by 23 publications

(15 citation statements)

References 30 publications

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“…AtAP1 : MYB24 -SRDX -bearing torenias exhibited wavy petals with a characteristic confi guration; this is a good example of the utilization of a fl oral organ-specifi c promoter for creating distinct fl ower phenotypes without causing unfavorable morphological and physiological changes in other organs (Sasaki et al 2011 ).…”

Section: Flower-specifi C Promoters In Ornamentalsmentioning

confidence: 99%

Promoters for Transgenic Horticultural Plants

Smirnova

Tishchenko²,

Ermakov

et al. 2014

Abiotic Stress Biology in Horticultural Plants

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Gene engineering provides an opportunity to obtain plants with either silenced or overexpressed target genes. This approach is frequently used to study various aspects of the biochemistry, physiology, and stress tolerance of horticultural plants. Selection of appropriate promoters to govern transcription of a transgenic construct is an important step in the development of effi cient genetic models. Here we present a brief overview of available literature data on the promoters investigated with the transgenic horticultural plants and some valuable information resources in this fi led.

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Section: Flower-specifi C Promoters In Ornamentalsmentioning

confidence: 99%

Promoters for Transgenic Horticultural Plants

Smirnova

Tishchenko²,

Ermakov

et al. 2014

Abiotic Stress Biology in Horticultural Plants

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“…Usage of floral organ-specific promoters, such as floral MADS-box gene promoters (Sasaki et al 2010;Sasaki et al 2011) or pigment biosynthesis genes in combination with the CpYGFP construct, may be effective for this purpose. Nondestructive and continuous monitoring of gene expressions during floral development will provide a new spatiotemporal perspective on the regulation of floral traits.…”

Section: Further Development and Improvement Of Fluorescent Flowersmentioning

confidence: 99%

Generation of fluorescent flowers exhibiting strong fluorescence by combination of fluorescent protein from marine plankton and recent genetic tools in <i>Torenia fournieri</i> Lind.

Sasaki

Kato

Mishima

et al. 2014

Plant Biotechnology

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Florescent proteins have been popularly used for studying genes and proteins of interest in various experiments at a cellular level, such as the analysis of intracellular localization and protein-protein interaction. However, the strength of fluorescence was insufficient for macro level observations of tissues or of the whole plant, and the fluorescent flowers that have been generated so far needed high-sensitive imaging equipment for the observation. Here we generated fluorescent Torenia flowers by the combined use of a high-performance fluorescent protein and the latest protein expression technologies, leading to the production of fluorescent proteins that can be easily and clearly observed. A coding sequence of a yellowish green fluorescent protein from the marine plankton Chiridius poppei (CpYGFP) was fused to the optimized sequences of the heat shock protein terminator and the 5′-untranslated region of the alcohol dehydrogenase gene of Arabidopsis to gain massive accumulation of the fluorescent protein. Strong fluorescence of CpYGFP was apparent in every part of the transgenic plant under the simple combination of a blue LED for excitation and an orange colored transparent acrylic filter for emission, while faint autofluorescence remained in the wild-type plants. By evaluating the combination of excitation wavelengths (excitation and emission filters) we were able to eliminate this undesired fluorescence. The fluorescent flowers could be used for ornamental purposes as well as for the analysis of fluorescent transgenic plants spatiotemporally in a nondestructive manner.

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“…For example, distinctive color patterns in torenia and Chrysanthemum morifolium flowers have been created as a result of reduced anthocyanin accumulation caused by the expression of the repressor of Arabidopsis TCP3 transcription factor [46]. Petals inside the flower buds also exhibited a distinct color pattern in transgenic torenia plants as a consequence of overexpression of the Arabidopsis MYB24 repressor with a transcriptional repression domain ( MYB24-SRDX ) [47]. Of interest is that transgenic torenia plants with variously modified traits have been produced by the collective transformation of about 50 transcription factors using CRES-T [48].…”

Section: Introductionmentioning

confidence: 99%

Frontiers of torenia research: innovative ornamental traits and study of ecological interaction networks through genetic engineering

et al. 2013

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Advances in research in the past few years on the ornamental plant torenia (Torenia spps.) have made it notable as a model plant on the frontier of genetic engineering aimed at studying ornamental characteristics and pest control in horticultural ecosystems. The remarkable advantage of torenia over other ornamental plant species is the availability of an easy and high-efficiency transformation system for it. Unfortunately, most of the current torenia research is still not very widespread, because this species has not become prominent as an alternative to other successful model plants such as Arabidopsis, snapdragon and petunia. However, nowadays, a more global view using not only a few selected models but also several additional species are required for creating innovative ornamental traits and studying horticultural ecosystems. We therefore introduce and discuss recent research on torenia, the family Scrophulariaceae, for secondary metabolite bioengineering, in which global insights into horticulture, agriculture and ecology have been advanced. Floral traits, in torenia particularly floral color, have been extensively studied by manipulating the flavonoid biosynthetic pathways in flower organs. Plant aroma, including volatile terpenoids, has also been genetically modulated in order to understand the complicated nature of multi-trophic interactions that affect the behavior of predators and pollinators in the ecosystem. Torenia would accordingly be of great use for investigating both the variation in ornamental plants and the infochemical-mediated interactions with arthropods.

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Utilization of a floral organ-expressing AP1 promoter for generation of new floral traits in Torenia fournieri Lind

Cited by 23 publications

References 30 publications

Promoters for Transgenic Horticultural Plants

Promoters for Transgenic Horticultural Plants

Generation of fluorescent flowers exhibiting strong fluorescence by combination of fluorescent protein from marine plankton and recent genetic tools in <i>Torenia fournieri</i> Lind.

Frontiers of torenia research: innovative ornamental traits and study of ecological interaction networks through genetic engineering

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