Utilization of a computer-controlled laboratory workstation (Biomek 1000) in routine radioimmunoassay laboratory

Hassan, Mithal; Issac, Daisy

doi:10.1016/0010-4825(90)90004-9

Cited by 3 publications

(1 citation statement)

References 1 publication

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“…However, the use of nondisposable tips can be more challenging than the adoption of disposable tips. Typically, clinical as well as forensic investigators have been cautious about equipping their robotic workstation with nondisposable tips for processing biological samples due to potential cross-contamination issues (10,(25)(26)(27)(28)(29)(31)(32)(33)(34)(35) despite the fact that nondisposable tips offer a high degree of precision when aspirating or dispensing liquids due to their specific manufacturing tolerances and engineered standards (7,(36)(37)(38). In addition, Teflon-coated stainless steel tips infrequently get plugged or damaged and therefore the tip replacement cost is minimal.…”

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confidence: 99%

Automated Processing of Forensic Casework Samples Using Robotic Workstations Equipped with Nondisposable Tips: Contamination Prevention

Frégeau

Lett

Elliott

et al. 2008

Journal of Forensic Sciences

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An automated process has been developed for the analysis of forensic casework samples using TECAN Genesis RSP 150/8 or Freedom EVO liquid handling workstations equipped exclusively with nondisposable tips. Robot tip cleaning routines have been incorporated strategically within the DNA extraction process as well as at the end of each session. Alternative options were examined for cleaning the tips and different strategies were employed to verify cross-contamination. A 2% sodium hypochlorite wash (1/5th dilution of the 10.8% commercial bleach stock) proved to be the best overall approach for preventing cross-contamination of samples processed using our automated protocol. The bleach wash steps do not adversely impact the short tandem repeat (STR) profiles developed from DNA extracted robotically and allow for major cost savings through the implementation of fixed tips. We have demonstrated that robotic workstations equipped with fixed pipette tips can be used with confidence with properly designed tip washing routines to process casework samples using an adapted magnetic bead extraction protocol.

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