Utilización de microorganismos de ambientes extremos y sus productos en el desarrollo biotecnológico

Oliart-Ros, Rosa María; Sánchez-Otero, María G.; Manresa-Presas, Ángeles

doi:10.29059/cienciauat.v11i1.556

Cited by 11 publications

(9 citation statements)

References 47 publications

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“…Most of the soluble enzymes are not suitable for industrial applications where harsh conditions, such as high temperatures or the presence of organic solvents, are generally required [1,2]. For this reason, immobilization techniques have emerged as an alternative to improve the catalytic properties and the operational stability of the enzymes, allowing enzymatic processes to be cost-effective compared to chemical ones.…”

Section: Introductionmentioning

confidence: 99%

Modulation of the Biocatalytic Properties of a Novel Lipase from Psychrophilic Serratia sp. (USBA-GBX-513) by Different Immobilization Strategies

et al. 2021

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In this work, the effect of different immobilization procedures on the properties of a lipase obtained from the extremophilic microorganism Serratia sp. USBA-GBX-513, which was isolated from Paramo soils of Los Nevados National Natural Park (Colombia), is reported. Different Shepharose beads were used: octyl-(OC), octyl-glyoxyl-(OC-GLX), cyanogen bromide (BrCN)-, and Q-Sepharose. The performance of the different immobilized extremophile lipase from Serratia (ESL) was compared with that of the lipase B from Candida antarctica (CALB). In all immobilization tests, hyperactivation of ESL was observed. The highest hyperactivation (10.3) was obtained by immobilization on the OC support. Subsequently, the thermal stability at pH 5, 7, and 9 and the stability in the presence of 50% (v/v) acetonitrile, 50% dioxane, and 50% tetrahydrofuran solvents at pH 7 and 40 °C were evaluated. ESL immobilized on octyl-Sepharose was the most stable biocatalyst at 90 °C and pH 9, while the most stable preparation at pH 5 was ESL immobilized on OC-GLX-Sepharose supports. Finally, in the presence of 50% (v/v) tetrahydrofuran (THF) or dioxane at 40 °C, ESL immobilized on OC-Sepharose was the most stable biocatalyst, while the immobilized preparation of ESL on Q-Sepharose was the most stable one in 40% (v/v) acetonitrile.

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Section: Introductionmentioning

confidence: 99%

Modulation of the Biocatalytic Properties of a Novel Lipase from Psychrophilic Serratia sp. (USBA-GBX-513) by Different Immobilization Strategies

et al. 2021

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“…Recent research points out the application of halophilic extremozymes in the production of biofuels. Since several halophiles are also alkaliphiles, their enzymes are of interest for the textile and detergent industries, and some have been explored as raw materials in the production of commercial enzymes, particularly proteases and amylases [5,[100][101][102][103][104][105].…”

Section: Halophile Extremozyme Applicationsmentioning

confidence: 99%

Kinetics of Halophilic Enzymes

Cira‐Chávez¹,

Guevara-Luna²,

YadiraSoto-Padilla³

et al. 2019

Kinetics of Enzymatic Synthesis

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Hypersaline environments are those with salt concentrations 9-10 times higher (30-35% of NaCl) than sea water (3.5% of NaCl). At high concentrations of soluble salts, cytoplasm-mainly of bacteria and archaea-is exposed to high ionic strength and achieves osmotic equilibrium by maintaining a cytoplasmic salt concentration similar to that of the surrounding media. Halophilic enzymes are extremozymes produced by halophilic microorganisms; they have similar characteristics to regular enzymes but different properties, mainly structural. Among these properties is a high requirement of salt for biological functions. Furthermore, the discovery of enzymes capable of degrading biopolymers offer a new perspective in the treatment of residues from oil deposits, under typically high conditions of salt and temperature, while giving valuable information on heterotrophic processes in saline environments.

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“…Las aguas mineromedicinales se distinguen por su composición química específica, en donde prevalecen las concentraciones de iones como sulfatos, fosfatos, nitratos, carbonatos, bicarbonatos, entre otras sustancias químicas, seleccionando de esta manera la existencia de especies microbianas capaces de sobrevivir en condiciones extremas de concentración de sal, pH y en algunos casos de temperaturas, lo que las hace particularmente atractivas en el estudio de microorganismos extremófilos con posibles aplicaciones biotecnológicas de interés para las industrias farmacéutica, alimenticia, química e incluso con aplicaciones en la biorremediación de compuestos nocivos para el medio ambiente (Bredholt et al, 1999;Ramírez et al, 2006;Viviano et al, 2011;Medina Ramírez et al, 2016;Oliart Ríos et al, 2016;Maraver et al, 2020). Dentro de las características que menos se han estudiado de las aguas mineromedicinales a nivel mundial, está la composición, diversidad, funciones, tamaño de la población bacteriana y los perfiles de resistencia a los antibióticos de estas.…”

Section: Introductionunclassified

Biodiversidad bacteriana en aguas de balnearios mineromedicinales de Ecuador y Venezuela

Leal

Rangel

Escudero

et al. 2023

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Las aguas mineromedicinales en Ecuador y Venezuela se utilizan desde hace siglos como medicamentos por la población de estos países. Sin embargo, la biodiversidad bacteriana es poco conocida. Por ello, el objetivo del trabajo fue conocer la biodiversidad bacteriana en el agua de balnearios mineromedicinales de Ecuador y Venezuela. Se tomaron muestras de agua en diversos punto de cada balneario. Los estudios microbiológicos se realizaron siguiendo los esquemas propuestos por MacFaddin, complementadas con las pruebas bioquímicas de las galería API y Microgen. En las aguas de los balnearios de Ecuador se identificaron un total de 284 colonias de los géneros Acidovorax, Acinetobacter, Actinomyces, Aeromonas, Alcaligenes, Bacillus, Brevibacterium, Brevundimonas, Budvicia, Burkholderia, Citrobacter, Corynebacterium, Edwardsiella, Ewingella, Flavobacterium, Kurthia, Micrococcus, Moraxella, Proteus, Pseudomonas, Psychrobacter, Ralstonia, Staphylococcus, Vibrio, Yersinia, Yokonella y Xenorhabdus. En las agua de los balnearios de Venezuela, se logró identificar 158 colonias de los géneros Aeromonas, Alcaligenes, Bacillus, Brevundimonas, Burkolderia, Chromobacterium, Citrobacter, Comamonas, Edwardsiella, Enterobacter, Klebsiella, Kluyvera, Moraxella, Plesiomonas, Proteus, Pseudomonas, Ralstonia, Shewanella, Staphylococcus, Weeksella y Vibrio. Se observo un claro predominio de las bacteria Gram negativas de la clase Gama proteobacterias en los balnearios mineromedicinales de ambos países.

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Utilización de microorganismos de ambientes extremos y sus productos en el desarrollo biotecnológico

Cited by 11 publications

References 47 publications

Modulation of the Biocatalytic Properties of a Novel Lipase from Psychrophilic Serratia sp. (USBA-GBX-513) by Different Immobilization Strategies

Modulation of the Biocatalytic Properties of a Novel Lipase from Psychrophilic Serratia sp. (USBA-GBX-513) by Different Immobilization Strategies

Kinetics of Halophilic Enzymes

Biodiversidad bacteriana en aguas de balnearios mineromedicinales de Ecuador y Venezuela

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