Utilização do teste de tetrazólio na avaliação da germinação e do vigor de sementes de algodão (Gossypium hirsutum L.), como um teste complementar ao teste padrão de germinação

Marenco, Ricardo Antonio; Santos, Carlos Eduardo Magalhães dos; Banci, Carlos Antonio; Calil, Antonio Cezar Pereira; Silva, Roberto Ferreira da; Santos, Vera Lúcia Machado dos

doi:10.17801/0101-3122/rbs.v14n2p155-159

Cited by 5 publications

(4 citation statements)

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“…The concentration of 0,075% is also recommended to other oleaginous seeds like cotton (SANTOS et al, 1992) and Lafoensia pacari (MENDONÇA et al, 2006), Butia capitata (FERNANDES et al, 2007), and beans feijão (MUASYA et al, 2002; besides Brassica rapa, Hordeum vulgare, Brassica napus, Avena sativa, Pastinaca sativa and Pisum sativum (POULSEN et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

Tetrazolium test to evaluate the viability of oil radish seeds

Nery

Pires

2015

Biosci. J.

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ABSTRACT:The use of tetrazolium test is important in the evaluation of seeds lot quality and it has been adopted for vigor and viability identification for several species. The interest on the production of oil radish is increasing since the seeds were considered a good source of oil for biofuel production. The development of the tetrazolium test methodology for seeds of this species can improve the seed quality control process, and additionally will provide information for the characterization of remaining seeds (dead or dormant) in the germination tests. To verify the ideal conditions to tetrazolium test was conducted two experiments. At the first, oil radish seeds cultivar CATI AL-1000, lots from 2001 and 2006 were submitted to imbibition between paper in water for 6 hours. After the longitudinal cut in the longest direction, the seeds were immersed in the tetrazolium solution at the concentrations of 0,075%, 0,5% and 1% at 25°C for 3h, 12h and 18h. In the second experiment, oil radish seeds cultivar CATI AL-1000 lot from 2005 and IPR 116 cultivar, lots from 2004 and 2005 were immersed in the concentrations of ,1%; 0,2%; 0,3% and 0,4% of tetrazolium solution for 12 hours at a 25°C, 30°C, 35°C and 40°C. At the first experiment was observed the necessity of test intermediate concentrations between 0,075% and 0,5%, since with 0,075% the seeds stained weakly and with 0,5% the test results, were overestimated. In the second experiment was observed that the 0,3% concentration at 30°C can be recommended for the utilization of tetrazolium test to evaluation of oil radish seeds viability.

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Section: Methodsmentioning

confidence: 99%

Tetrazolium test to evaluate the viability of oil radish seeds

Nery

Pires

2015

Biosci. J.

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“…Porém, aqueles resultados podem ter sido mascarados pelo excessivo desenvolvimento de fungos, observado durante a condução dos testes. Outros autores também verificaram diferenças maiores que 5% entre os resultados do teste de tetrazólio e do teste padrão de germinação em sementes de soja, amendoim e algodão, quando estas se encontraram infectadas por fungos (França Neto et al, 1998;Bittencourt, 1995;Santos et al, 1992).…”

Section: Correlaçãounclassified

Avaliação da qualidade fisiológica de sementes de mamoneira (Ricinus communis L.) pelo teste de tetrazólio

Gaspar-Oliveira¹,

Martins

Nakagawa

2010

Rev. bras. sementes

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RESUMO -Objetivou-se com esse trabalho estabelecer parâmetros para a avaliação da qualidade fisiológica de sementes de mamoneira (Ricinus communis L.) pelo teste de tetrazólio. Avaliaramse cinco lotes de sementes quanto à viabilidade e ao vigor pelo teste de tetrazólio e os resultados comparados aos obtidos nos testes de germinação em areia, germinação em papel, primeira contagem da germinação em papel, emergência de plântulas em campo, índice de velocidade de emergência, classificação do vigor de plântulas, comprimento das plântulas, massa da matéria seca de plântulas e envelhecimento acelerado. O delineamento experimental empregado foi o inteiramente casualizado, e a comparação de médias realizada pelo teste de Tukey a 5% de probabilidade. Os resultados de viabilidade do teste de tetrazólio se correlacionaram com o teste de germinação em areia, com a emergência em campo e com o comprimento de plântulas, e os de vigor do teste de tetrazólio com a classificação do vigor de plântulas. Concluiu-se que os parâmetros estabelecidos são eficientes para a avaliação da viabilidade e promissores para a avaliação do vigor de sementes de mamoneira pelo teste de tetrazólio.Termos para indexação: viabilidade, vigor, mamona, metodologia. EVALUATION OF THE PHYSIOLOGICAL QUALITY OF CASTOR BEAN SEED (Ricinus communis L.) USING THE TETRAZOLIUM TESTABSTRACT -The object of this study was to establish parameters to evaluate the physiological quality of castor bean seeds (Ricinus communis L.) using the the tetrazolium test. Five seed lots were evaluated for viability and vigor by the tetrazolium test and the results compared with the germination test in sand, on paper, first count of the germination test on paper, seedling emergence in the field, emergence speed index, seedling vigor classification, seedling length and dry matter, and accelerated ageing. The statistical design was completely randomized and the comparison of means was by the Tukey test at the 0.05 level of probability. The tetrazolium test viability results were correlated with the germination test in sand, with seedling emergence in the field and with seedling length, and tetrazolium vigor was correlated with seedling vigor classification. It was concluded that the parameters established are efficient for estimating castor bean seed viability and are promising for evaluating castor bean seed vigor using the tetrazolium test.

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“…Also, it is an alternative for species whose seeds have dormancy or slow germination, especially native and forest species (Lamarca and Barbedo, 2014). It has also been used efficiently in seeds from agricultural crops such as soybean (Pereira et al, 2019), chickpeas (Paraíso et al, 2019), beans (Bhering et al, 1999), cotton (Santos et al, 1992), corn (Chamma and Novembre, 2007), coffee (Dias and Silva, 1986) and barley (Grzybowski et al, 2012). In forage species such as Brachiaria spp.…”

Section: Introductionmentioning

confidence: 99%

Adequacy of the tetrazolium test to evaluate the viability of Jatropha curcas L. seeds

et al. 2019

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The tetrazolium test has been recommended for evaluating the viability of seeds with slow and ununiform germination, especially agroforestry species. The aim of this study was to propose a methodology to evaluate the viability of J. curcas L. seeds using the tetrazolium test and to generate visual aids to support the interpretation of the test. In the first trial, methods of seed preparation and staining were evaluated. The most promising methodologies were used to conduct the second test, which consisted of the evaluation of different concentrations (0.10 and 0.25%) and times (2 and 3 h) of immersion of the seeds in the tetrazolium solution, using five lots. The seeds were analyzed individually and classified as viable or non-viable. At the same time, germination, first count, emergence and seedling emergence speed index tests were conducted. The tetrazolium test is efficient for evaluating the viability of seeds of J. curcas L. providing results correlated with germination and seedling emergence. Pre-soaked seeds should have their integument removed and then sectioned longitudinally and medially lengthwise for immersion in 0.10% solution of 2,3,5-triphenyl chloride tetrazolium for three hours at 35 °C.

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Utilização do teste de tetrazólio na avaliação da germinação e do vigor de sementes de algodão (Gossypium hirsutum L.), como um teste complementar ao teste padrão de germinação

Cited by 5 publications

References 0 publications

Tetrazolium test to evaluate the viability of oil radish seeds

Tetrazolium test to evaluate the viability of oil radish seeds

Avaliação da qualidade fisiológica de sementes de mamoneira (Ricinus communis L.) pelo teste de tetrazólio

Adequacy of the tetrazolium test to evaluate the viability of Jatropha curcas L. seeds

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