Utility of the national embryo morphology data collection by the Society for Assisted Reproductive Technologies (SART): correlation between day-3 morphology grade and live-birth outcome

Vernon, Michael; Stern, Judy E.; Ball, G.D.; Wininger, David; Mayer, Jacob; Racowsky, Catherine

doi:10.1016/j.fertnstert.2011.02.008

Cited by 37 publications

(30 citation statements)

References 11 publications

(22 reference statements)

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“…There is e.g. a need for frequent observations to select the best in-vitro produced embryos for transfer, because better embryonic quality means higher chance for implantation and ultimately pregnancy [4][5][6]. In practice, the number of observations used in clinical IVF on human and animal embryos is therefore a compromise between a wish to keep the embryos undisturbed (with respect to light, temperature and pH) and to have as many observations as possible to facilitate optimal selection for transfer.…”

Section: Introductionmentioning

confidence: 99%

Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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Purpose To assess irradiance and total energy dose from different microscopes during the in-vitro embryonic developmental cycle in mouse and pig and to evaluate its effect on embryonic development and quality in pig. Method Spectral scalar irradiance (380-1050 nm) was measured by a fiber-optic microsensor in the focal plane of a dissection microscope, an inverted microscope and a timelapse incubation system. Furthermore, the effect of three different red light levels was tested in the time-lapse system on mouse zygotes for 5 days, and on porcine zona-intact and zona-free parthenogenetically activated (PA) embryos for 6 days. Results The time-lapse system used red light centered at 625 nm and with a lower irradiance level as compared to the white light irradiance levels on the dissection and inverted microscopes, which included more energetic radiation <550 nm. Even after 1000 times higher total energy dose of red light exposure in the time-lapse system, no significant difference was found neither in blastocyst development of mouse zygotes nor in blastocyst rates and total cell number of blastocysts of porcine PA embryos. Conclusions Our results indicate that red light (625 nm, 0.34 W/ m 2 ) used in the time-lapse incubation system does not decrease the development and quality of blastocysts in both mouse zygotes and porcine PA embryos (both zona-intact and zona-free).

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Section: Introductionmentioning

confidence: 99%

Effect of red light on the development and quality of mammalian embryos

Pedersen

Liu

et al. 2014

J Assist Reprod Genet

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“…This has been recently confirmed in the SART register, with live-birth rates ranging from 44.9 to 34.9 or 21.3% using two good versus two fair or two poor embryos [18] . Accordingly, selection of the best embryo(s) to transfer is one of the most important steps in the IVF procedure and considerable efforts have been made to develop more accurate methods, either invasive or, preferably, non-invasive, by which to conduct this selection process.…”

Section: Discussionmentioning

confidence: 61%

“…Indeed, embryo morphology has long been known to be strongly associated with ART success rates [15][16][17] . This has recently been confirmed in a large multicenter trial, and the association was found to be independent of maternal age [18] . Pregnancy rates were found to be twofold higher with top quality (TQ) than poor quality embryos [18] .…”

Section: Introductionmentioning

confidence: 52%

“…This has recently been confirmed in a large multicenter trial, and the association was found to be independent of maternal age [18] . Pregnancy rates were found to be twofold higher with top quality (TQ) than poor quality embryos [18] . However, pregnancy is possible even in cycles using poor quality embryos (grade 4 or 5 morphology) [19] .…”

Section: Introductionmentioning

confidence: 52%

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Congenital Malformations, Chromosomal Abnormalities and Perinatal Results in IVF/ICSI Newborns Resulting from Very Poor Quality Embryos: A Case-Control Study

Mendoza

Pérez

Santos

et al. 2014

Gynecol Obstet Invest

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Aims: To explore whether the transfer of very poor quality (VPQ) embryos is associated with an increase in congenital malformations or perinatal problems. Methods: In this retrospective case-control study, 74 children conceived by in vitro fertilization (IVF) and/or intracytoplasmic sperm injection (ICSI) resulting exclusively from the transfer of VPQ embryos were compared with 1,507 children born after the transfer of top morphological quality (TQ) embryos over the same period of time in the same centers. Results: The prevalence of birth defects in children resulting from VPQ embryos was 1.35% (1/74), similar to the 1.72% (26/1,507) when only TQ embryos were transferred; the rate of chromosomal abnormalities detected was also similar (0.0 vs. 0.4%), as was perinatal mortality. After correcting for multiplicity (higher in the TQ group), the aforementioned parameters remained similar in the two groups. Conclusion: Congenital malformations and perinatal complications do not seem to be more common in children born after transfer of VPQ embryos in IVF/ICSI cycles. Given our preliminary data, which need to be confirmed in much larger studies, when only VPQ embryos are available for transfer in IVF/ICSI cycles, we do not believe that they should be discarded with the intention of avoiding birth defects or perinatal complications.

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“…Grading of day 3 embryos comprises cell number, degree of fragmentation, cell symmetry and presence or absence of cytoplasmic pitting, and has been shown to predict ART outcomes [1][2][3]. Better morphology grade embryos have been associated with significantly improved implantation rates and live birth rates [4,5].…”

Section: Introductionmentioning

confidence: 99%

Embryo quality but not pronuclear score is associated with clinical pregnancy following IVF

Berger

Zapantis

Merhi

et al. 2014

J Assist Reprod Genet

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Purpose The association between pronuclear (PN) scoring of embryos from assisted reproductive technology (ART) and clinical pregnancy remains controversial. We hypothesized that embryos with PNs scored on the day of fertilization check offer better embryo selection on day 3 and higher CPR compared to non-PN scored embryos. Methods Patients (19-46 years) undergoing IVF/ICSI cycles at Montefiore's Institute for Reproductive Medicine and Health between January 2006 and December 2009 were included in our study. We analyzed fresh day 3 cycles only with autologous oocytes and partner's fresh sperm (n=344). A total of 1,899 embryos were included. We compared CPR from non-PN scored embryos (Group 1, n=835) with PN scored embryos (Group 2, n=1,064). Composite scores by patient were developed based on embryo disposition. We also assessed traditional embryo grading derived from cell number, fragmentation and cell symmetry. Data analysis included chi square and t test to determine if PN scoring was associated with improved CPR, and to compare the additional variables.Results CPR between Group 1 and Group 2 were not different (p=0.91). CPR was significantly associated with female age, number of mature oocytes retrieved, number of day 3 embryos and grade of embryos transferred on day 3 (p<0.05). Conclusion PN scoring was not associated with improved CPR in day 3 embryo transfers. Mean grade of transferred embryos continues to be a well-established, independent predictor of CPR. We conclude that further refinement of embryo grading by PN scoring is not beneficial.

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Utility of the national embryo morphology data collection by the Society for Assisted Reproductive Technologies (SART): correlation between day-3 morphology grade and live-birth outcome

Cited by 37 publications

References 11 publications

Effect of red light on the development and quality of mammalian embryos

Effect of red light on the development and quality of mammalian embryos

Congenital Malformations, Chromosomal Abnormalities and Perinatal Results in IVF/ICSI Newborns Resulting from Very Poor Quality Embryos: A Case-Control Study

Embryo quality but not pronuclear score is associated with clinical pregnancy following IVF

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