Utility of the BACTEC Myco/F lytic medium for the detection of mycobacteria in blood

Martínez-Sánchez, Lucía; Ruiz-Serrano, Jesús; Bouza, Emilio; Torres, Lorenzo; Díaz, Marisol; Alcalá, Luís; Rodríguez‐Créixems, Marta

doi:10.1016/s0732-8893(00)00202-9

Cited by 8 publications

(6 citation statements)

References 15 publications

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“…The results showed that the MycoF/Lytic system was generally faster than lysis–centrifugation for both types of specimen, whether for MAC or MTBC isolates, with a decrease in median time required for detection of 33.5–22 days (decreasing average, 34%; range, 7–62%). A previous study performed with blood specimens showed a reduction in the time required for diagnosis of 61%[8]. Other studies have shown the utility of the MycoF/Lytic system for the diagnosis of DMI from blood specimens, whether for MAC infections (median time range, 10–16 days) [8,13,14] or MTBC infections (median time range, 19–26 days) [8,15].…”

Section: Discussionmentioning

confidence: 99%

“…From January 1990 to February 1997, BM and blood specimens were processed by lysis–centrifugation [8], using Lowenstein–Jensen medium (with and without pyruvate) and Middlebrook 7H9 broth as culture media. Briefly, 8–10 mL of blood was inoculated into an average of three BACTEC PLUS Aerobic/F* bottles (Becton Dickinson, Sparks, MD, USA).…”

Section: Cultures and Identificationmentioning

confidence: 99%

“…During the second period (February 1997 onwards), both specimen types were cultured in MycoF/Lytic bottles (Becton Dickinson) according to standard procedures [8].…”

Section: Cultures and Identificationmentioning

confidence: 99%

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Evaluation of bone marrow and blood cultures for the recovery of mycobacteria in the diagnosis of disseminated mycobacterial infections

Pacios

Alcalá

Ruiz‐Serrano

et al. 2004

Clinical Microbiology and Infection

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This study evaluated the validity of bone marrow (BM) and blood specimens for the diagnosis of disseminated mycobacterial infections (DMIs). From 1990 to February 1997, all specimens were processed with the lysis-centrifugation procedure; thereafter (until December 2001), they were processed with the BACTEC Myco/F Lytic system. Twenty-three paired BM-blood specimens with mycobacteria in at least one specimen were studied from 23 patients. The strains isolated were 14 Mycobacterium avium complex (MAC) and nine M. tuberculosis complex (MTBC). Blood specimens had a statistically significant greater sensitivity for the isolation of MAC than BM (100% vs. 57.1%, respectively), whereas sensitivity for the isolation of MTBC was equal for the two specimen types (66.7%). Although not statistically significant, the times required to detect mycobacteria from blood specimens were lower than those from BM in the MycoF/Lytic system. Overall, blood cultures represented a more sensitive and less invasive alternative to BM cultures for the diagnosis of disseminated mycobacteriosis caused by MAC, especially when the MycoF/Lytic system was used, but provided no advantage for the diagnosis of DMI caused by MTBC.

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Section: Discussionmentioning

confidence: 99%

Section: Cultures and Identificationmentioning

confidence: 99%

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Evaluation of bone marrow and blood cultures for the recovery of mycobacteria in the diagnosis of disseminated mycobacterial infections

Pacios

Alcalá

Ruiz‐Serrano

et al. 2004

Clinical Microbiology and Infection

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show abstract

“…The whole process is often conducted by the person who collected the specimen10. Studies have shown that Myco/F lytic system is not only rapid and simple but also safe and highly reliable diagnostic method for the detection of mycobacteria in blood111213. Up to now, only one study has evaluated the performance of Myco/F lytic system for recovery of M. tuberculosis from pleural fluid, and the results showed that the Myco/F lytic system can be an alternative to the MGIT system for diagnosing pleural TB14.…”

mentioning

confidence: 99%

Evaluation of the efficacy of Myco/F lytic system, MGIT960 system and Lowenstein-Jensen medium for recovery of Mycobacterium tuberculosis from sterile body fluids

Wang

Yang

Zhu

et al. 2016

Sci Rep

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The diagnosis of extrapulmonary tuberculosis (EPTB) is challenging due to non-specific symptoms, invasive approach for specimen collection and most importantly, the paucibacillary status. The objective of this assay was to evaluate the efficacy of Myco/F lytic system, BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system and Lowenstein-Jensen (L-J) medium for recovery of bacilli from sterile body fluids. 214 specimens (114 pleural fluid and 100 pus) from clinically diagnosed EPTB patients were collected and subjected to Ziehl-Neelsen (ZN) smear microscopy, L-J culture, MGIT 960 culture and Myco/F lytic culture.103 out of the 214 sterile body fluid samples yielded positive culture outcomes by any of the three methods. Among all the culture positive specimens, the recovery rate was 86.41% for Myco/F lytic, 75.73% for MGIT 960, and 42.72% for L-J medium. The mean time to positivity (TTP) was 27.06 ± 8.03 days for Myco/F lytic, 22.20 ± 7.84 days for MGIT960 and 42 ± 8.84 days for L-J medium. The rates of contamination were 6.54%, 3.74% and 2.80% for Myco/F lytic, MGIT960 and L-J medium respectively. Both Myco/F lytic and MGIT960 system were superior to L-J medium for recovery of bacilli from sterile body fluids. Myco/F lytic system was more favorable than MGIT960 regarding recovery rate and cost-effectiveness, thus can be considered as a promising alternative to MGIT960 system for diagnosing EPTB.

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“…Semiautomated liquid culture systems, such as the BACTEC radiometric method or MB/Bact and MGIT systems, are able to detect mycobacterial slow growers within 10 to 14 days. While several of these growth detection methods have demonstrated good performance comparable to standard methods (23,26), the necessity for a heavy and expensive apparatus limits their use in poor countries. Moreover, additional tests may be necessary to finally identify the mycobacterial species.…”

Section: Discussionmentioning

confidence: 99%

Specificities and Functions of the recA and pps1 Intein Genes of Mycobacterium tuberculosis and Application for Diagnosis of Tuberculosis

et al. 2002

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The worldwide recrudescence of tuberculosis and the widespread appearance of antibiotic resistance have strengthened the need for rapid and specific diagnostic tools. The prevailing microbiological identification of Mycobacterium tuberculosis, the causative agent of tuberculosis, which implies the use of in vitro cultures and acid-fast staining microscopy, is time-consuming. Detection of M. tuberculosis directly in clinical samples through PCR amplification of mycobacterium-specific genes, designed to shorten diagnostic delay, demonstrated reliability and high sensitivity. However, the quality of the diagnosis depends on the specificity of the target sequence for M. tuberculosis complex strains. In the present study, we demonstrated the specificity of recA and pps1 inteins for this complex and thus the feasibility of using intein-coding sequences as a new target for PCR diagnosis. Indeed, the recA and pps1 genes of 36 clinical isolates of M. tuberculosis and 10 field strains of M. bovis were found to be interrupted by an intein sequence at the RecA-a and Pps1-b sites, respectively, while a large number of nontuberculous mycobacterial species failed to demonstrate these insertions. Besides, the MtuPps1, which was cloned and expressed in Escherichia coli, was shown to possess an endonuclease activity. The intein cleaves the 40-bp sequence spanning the intein insertion site Pps1-b in the inteinless pps1 gene. In addition to the PCR amplification of recA and pps1 intein genes as a tool for diagnosis, the specific endonuclease activity could represent a new molecular approach to identify M. tuberculosis.Tuberculosis remains a major public health problem in developing countries. Despite the decline in incidence observed in the 1980s, resurgence has recently occurred. Furthermore, the recrudescence of infections with Mycobacterium tuberculosis and the emergence of multidrug-resistant strains represent a serious public health threat in developed countries, where the incidence of tuberculosis is related to immunodeficient patients, homeless people, and the advancing age of the population (World Health Organization web site at http://www .who.int/health-topics/tb.htm).This worldwide resurgence of tuberculosis has strengthened the need for rapid and specific diagnostic tools to control its spread. Delay in diagnosis results, first, in late initiation of antitubercular therapy and, second, in prolonged transmission of the infection.Until recently, the diagnosis of tuberculosis was based on clinical features and microbiological assays, radiological examinations, immunological tests, microscopy identification, or in vitro cultures (37). Acid-fast staining microscopy of specimens combined with isolation and culture of the bacilli remains the "gold standard" method to specifically identify mycobacteria. Because of the low growth rate of M. tuberculosis, this method is time-consuming, and the diagnosis can take up to 8 weeks.More recently, molecular approaches have been designed to ensure early detection of M. tuberculosis in cl...

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Utility of the BACTEC Myco/F lytic medium for the detection of mycobacteria in blood

Cited by 8 publications

References 15 publications

Evaluation of bone marrow and blood cultures for the recovery of mycobacteria in the diagnosis of disseminated mycobacterial infections

Evaluation of bone marrow and blood cultures for the recovery of mycobacteria in the diagnosis of disseminated mycobacterial infections

Evaluation of the efficacy of Myco/F lytic system, MGIT960 system and Lowenstein-Jensen medium for recovery of Mycobacterium tuberculosis from sterile body fluids

Specificities and Functions of the recA and pps1 Intein Genes of Mycobacterium tuberculosis and Application for Diagnosis of Tuberculosis

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