Utility of T2 mapping and dGEMRIC for evaluation of cartilage repair after allograft chondrocyte implantation in a rabbit model

Watanabe, Atsuya; Sasho, Takahisa; Yamaguchi, Satoshi; Saito, M.; Akagi, Ryuichiro; Muramatsu, Yusuke; Mukoyama, S.; Katsuragi, J.; Akatsu, Yorikazu; Fukawa, Taisuke; Okubo, Toshiyuki; Osone, Fumio; Takahashi, Kazuhisa

doi:10.1016/j.joca.2014.10.012

Cited by 16 publications

(13 citation statements)

References 44 publications

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“…Assim, a RM destacou-se como uma importante ferramenta diagnóstica, uma vez que permitiu a detecção de alterações no processo de reparação dos enxertos osteocondrais. A RM é reconhecida como um método não invasivo para examinar patologias articulares e uma referência efetiva para avaliar a reparação em cartilagens (Goebel et al 2012, Rodríguez--Merchán 2012, Bekkers et al 2013, Endo et al 2015, Obara et al 2015. Além disso, a RM também pode ser útil na identificação de lesões ósseas ocultas à radiografia convencional, incluindo as contusões ósseas e fraturas osteocondrais, que representam sítios de hiperemia, edema ou hemorragia óssea (Grossi et al 2001, Kido et al 2014.…”

Section: Discussionunclassified

“…Constitui-se um exame não radio-ionizante e não invasivo de escolha para se avaliar a lesão e os processos de reparação articulares. Permite a visualização detalhada dos tecidos intra-articulares, fornecendo contraste diferenciado entre os tecidos moles em diferentes ponderações de imagem e com capacidade multiplanar (Muglia et al 2001, Efe et al 2012, Blackman et al 2013, Endo et al 2015. A partir da utilização de técnicas específicas para a região articular, a RM fornece informações sobre as alterações de sinal do tecido cartilaginoso, da morfologia da superfície articular e do osso subcondral (Rodrigues & Camanho 2010, Kido et al 2014.…”

Section: Introductionunclassified

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Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

et al. 2017

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ABSTRACT. - This study aimed to determine the characteristics and applicability of magnetic resonance imaging (MRI) in the evaluation of autogenous osteochondral graft in intact or macerated format, with or without insulin-like growth factor type 1 (IGF-1) used in repair of cartilage lesions induced in rabbits. Nine New Zealand rabbits were used, in which 18 stifle joints underwent grafting procedure in the femoral trochlear groove. These were divided into four groups, referred as intact osteochondral graft + IGF-1 (n=5), intact osteochondral graft + saline solution (n=4), macerated osteochondral graft + IGF-1 (n=5) and macerated osteochondral graft + saline solution (n=4). Animals were euthanized 12 weeks after surgery and the joints were subjected to MRI using a high magnetic field scanner of 1.5 Tesla. In addition, samples of grafting sites were subjected to anatomopathological examination. The MRI was effective as a noninvasive method to evaluate the repair tissue in osteochondral grafts in articular cartilage of the femur of rabbits by providing complementary data to macroscopic and histological examinations. Through these images and anatomopathological examinations satisfactory results were observed in relation to the repair process of autogenous osteochondral grafts in cartilage of rabbits, regardless of its format or the addition of IGF-1. RESUMO.-Este trabalho teve o objetivo de determinar as características e a aplicabilidade do exame de ressonância magnética na avaliação de enxerto ostecondral autógeno, em formato íntegro ou macerado, associado ou não ao fator de crescimento semelhante à insulina tipo 1 (IGF-1), utilizado no reparo de lesões induzidas na cartilagem articular de coelhos. Foram utilizados 9 coelhos da linhagem Nova Zelândia, em que as 18 articulações fêmoro-tíbio-patelares foram submetidas à enxertia osteocondral autógena no sulco troclear femoral. Estas foram divididas em quatro grupos, denominados como enxerto osteocondral íntegro + IGF-1 (n=5), enxerto osteocondral íntegro + solução fisioló-gica (n=4), enxerto osteocondral macerado + IGF-1 (n=5) e enxerto osteocondral macerado + solução fisiológica (n=4). Os animais foram eutanasiados em 12 semanas após a cirurgia e as articulações foram submetidas ao exame de ressonância magnética utilizando um aparelho scanner de 1,5 Tesla de alto campo magnético. Além disso, amostras dos locais de enxertia foram submetidas aos exames anatomopatológicos. O exame de ressonância magnética mostrou--se eficaz como um método não invasivo para avaliação do tecido de reparação em enxertos osteocondrais na cartilagem articular do fêmur de coelhos, fornecendo dados complementares aos exames macroscópicos e histológicos. Por

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Section: Discussionunclassified

Section: Introductionunclassified

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

et al. 2017

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“…Articular cartilage defect caused by trauma or degenerative pathology is major challenge because of the poor intrinsic healing potential of cartilage. Many attempts including bone marrow stimulation technique, allograft or autograft transplantation, tissue engineering, and autologous chondrocyte implantation procedures have been conducted to repair cartilage defects 27 28 29 . However, no techniques in the literature have been reported to be able to regenerate functional cartilage similar to the quality of native cartilage.…”

Section: Discussionmentioning

confidence: 99%

A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration

Meng

Man

Dai

et al. 2015

Sci Rep

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Articular cartilage injury is still a significant challenge because of the poor intrinsic healing potential of cartilage. Stem cell-based tissue engineering is a promising technique for cartilage repair. As cartilage defects are usually irregular in clinical settings, scaffolds with moldability that can fill any shape of cartilage defects and closely integrate with the host cartilage are desirable. In this study, we constructed a composite scaffold combining mesenchymal stem cells (MSCs) E7 affinity peptide-modified demineralized bone matrix (DBM) particles and chitosan (CS) hydrogel for cartilage engineering. This solid-supported composite scaffold exhibited appropriate porosity, which provided a 3D microenvironment that supports cell adhesion and proliferation. Cell proliferation and DNA content analysis indicated that the DBM-E7/CS scaffold promoted better rat bone marrow-derived MSCs (BMMSCs) survival than the CS or DBM/CS groups. Meanwhile, the DBM-E7/CS scaffold increased matrix production and improved chondrogenic differentiation ability of BMMSCs in vitro. Furthermore, after implantation in vivo for four weeks, compared to those in control groups, the regenerated issue in the DBM-E7/CS group exhibited translucent and superior cartilage-like structures, as indicated by gross observation, histological examination, and assessment of matrix staining. Overall, the functional composite scaffold of DBM-E7/CS is a promising option for repairing irregularly shaped cartilage defects.

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“…Thus, MRI could be useful both during bioreactor cultivation and upon surgical implantation in a noninvasive manner . A recent study demonstrated the effectiveness of quantitative MRI for evaluating the quality of cartilage repair by a 3D scaffold implanted in osteochondral lesions, following allograft chondrocyte implantation over time . It has been used for biomechanical measurements in cartilage and related joint tissues and, in comparison with other modalities, MRI provides an extensive range of functional measures at the tissue level .…”

Section: Available Imaging Techniquesmentioning

confidence: 99%

Three‐dimensional imaging technologies: a priority for the advancement of tissue engineering and a challenge for the imaging community

Teodori

Crupi

Costa

et al. 2016

Journal of Biophotonics

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Tissue engineering/regenerative medicine (TERM) is an interdisciplinary field that applies the principle of engineering and life sciences to restore/replace damaged tissues/organs with in vitro artificially-created ones. Research on TERM quickly moves forward. Today newest technologies and discoveries, such as 3D-/bio-printing, allow in vitro fabrication of ex-novo made tissues/organs, opening the door to wide and probably never-ending application possibilities, from organ transplant to drug discovery, high content screening and replacement of laboratory animals. Imaging techniques are fundamental tools for the characterization of tissue engineering (TE) products at any stage, from biomaterial/scaffold to construct/organ analysis. Indeed, tissue engineers need versatile imaging methods capable of monitoring not only morphological but also functional and molecular features, allowing three-dimensional (3D) and time-lapse in vivo analysis, in a non-destructive, quantitative, multidimensional analysis of TE constructs, to analyze their pre-implantation quality assessment and their fate after implantation. This review focuses on the newest developments in imaging technologies and applications in the context of requirements of the different steps of the TERM field, describing strengths and weaknesses of the current imaging approaches.

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Utility of T2 mapping and dGEMRIC for evaluation of cartilage repair after allograft chondrocyte implantation in a rabbit model

Abstract: T2 mapping and dGEMRIC were both effective for evaluating tissue repair after allograft chondrocyte implantation. ΔR1 values of dGEMRIC represented good correlation with histologically and biochemically even at early stages after the implantation.

Cited by 16 publications

References 44 publications

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

Ressonância magnética na avaliação de enxerto osteocondral autógeno na cartilagem articular de coelhos

A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration

Three‐dimensional imaging technologies: a priority for the advancement of tissue engineering and a challenge for the imaging community

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