Utility of Stable Isotope and Cytochrome Oxidase I Gene Sequencing Analyses in Inferring Origin and Authentication of Hairtail Fish and Shrimp

Kim, Hyung Jun; Kumar, K. Suresh; Sy, Hwang; Kang, Byung Chul; Hb, Moon; Shin, Kyung‐Hoon

doi:10.1021/acs.jafc.5b01469

Cited by 18 publications

(3 citation statements)

References 35 publications

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“…Oftentimes, fish and fish products lack external morphological features like fin position, body shape, scale size, or color pattern, which makes visually identifying one species from the other a difficult task (Teletchea, 2009). In addition, phenotypic similarities among some fish types/species further complicate the determination of origin and species identification (Kim et al., 2015). Therefore, (1) increased demand, (2) complexity of the supply chain, and (3) inability to determine species once morphological characteristics are removed make fish a prime target for fraudulent activities.…”

Section: Introductionmentioning

confidence: 99%

Twenty‐three years of PCR‐based seafood authentication assay development: What have we learned?

Singh,

Young,

Hellberg

et al. 2024

Comp Rev Food Sci Food Safe

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Seafood is a prime target for fraudulent activities due to the complexity of its supply chain, high demand, and difficult discrimination among species once morphological characteristics are removed. Instances of seafood fraud are expected to increase due to growing demand. This manuscript reviews the application of DNA‐based methods for commercial fish authentication and identification from 2000 to 2023. It explores (1) the most common types of commercial fish used in assay development, (2) the type of method used, (3) the gene region most often targeted, (4) provides a case study of currently published assays or primer‐probe pairs used for DNA amplification, for specificity, and (5) makes recommendations for ensuring standardized assay‐based reporting for future studies. A total of 313 original assays for the detection and authentication of commercial fish species from 191 primary articles published over the last 23 years were examined. The most explored DNA‐based method was real‐time polymerase chain reaction (qPCR), followed by DNA sequencing. The most targeted gene regions were cytb (cytochrome b) and COI (cytochrome c oxidase 1). Tuna was the most targeted commercial fish species. A case study of published tuna assays (n = 19) targeting the cytb region found that most assays were not species‐specific through in silico testing. This was conducted by examining the primer mismatch for each assay using multiple sequence alignment. Therefore, there is need for more standardized DNA‐based assay reporting in the literature to ensure specificity, reproducibility, and reliability of results. Factors, such as cost, sensitivity, quality of the DNA, and species, should be considered when designing assays.

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Section: Introductionmentioning

confidence: 99%

Twenty‐three years of PCR‐based seafood authentication assay development: What have we learned?

Singh,

Young,

Hellberg

et al. 2024

Comp Rev Food Sci Food Safe

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“…DNA-based techniques may overcome these shortcomings because of the unparalleled stability of DNA . Among the DNA techniques, the 12S rRNA and 16S rRNA genes and the entire tRNA Val region − together with the cytochrome oxidase I (COI) , and cytochrome b genes have been considered to be useful molecular biomarkers for crustacean species discrimination. Nevertheless, selection at the molecular level is measured by analysis of DNA sequence variations only, and analyses based on protein sequences were complements of DNA-based methods …”

Section: Introductionmentioning

confidence: 99%

Identification of Peptide Biomarkers for Discrimination of Shrimp Species through SWATH-MS-Based Proteomics and Chemometrics

Zhang

et al. 2018

J. Agric. Food Chem.

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Incorrect labeling and adulteration of shrimp occurs due to interspecies similarities and carapace removal during processing. This study attempted to identify three related commercial shrimp species of the order Decapoda: Marsupenaeus japonicus, Fenneropenaeus chinensis, and Litopenaeus vannamei. All measurable trypsin-digested peptides in the individual shrimp were detected using ultrahigh-performance liquid chromatography quadrupole time-of-flight (UPLC-Q-TOF) mass spectrometry with sequential window acquisition of all theoretical fragment ion spectra (SWATH) data-independent acquisition. Further analysis of peptide biomarkers was carried out with an orthogonal partial least-squares discriminant analysis (OPLS-DA) model. BLAST was used for species-specific analysis. Subsequently, multiple reaction monitoring (MRM) methods were developed for sensitivity and selectivity screening of the selected peptides, and 27 were identified as biomarkers allowing rapid and accurate discrimination of shrimp species without high-resolution mass spectrometry or statistical model building. These strategies could be applied in authentication of other products containing highly homologous proteomes.

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“…Mo,109 Ag/107 Ag,114 Cd/113 Cd,114 Cd/112 Cd,114 Cd/111 Cd,114 Cd/110 Cd,113 Cd/ 112 Cd,13 Cd/111 Cd,113 Cd/ 110 Cd,112 Cd/111 Cd,112 Cd/ 110 Cd,111 Cd/ 110 Cd…”

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Isotope Fingerprinting as a Backup for Modern Safety and Traceability Systems in the Animal-Derived Food Chain

et al. 2023

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In recent years, due to the globalization of food trade and certified agro-food products, the authenticity and traceability of food have received increasing attention. As a result, opportunities for fraudulent practices arise, highlighting the need to protect consumers from economic and health damages. In this regard, specific analytical techniques have been optimized and implemented to support the integrity of the food chain, such as those targeting different isotopes and their ratios. This review article explores the scientific progress of the last decade in the study of the isotopic identity card of food of animal origin, provides the reader with an overview of its application, and focuses on whether the combination of isotopes with other markers increases confidence and robustness in food authenticity testing. To this purpose, a total of 135 studies analyzing fish and seafood, meat, eggs, milk, and dairy products, and aiming to examine the relation between isotopic ratios and the geographical provenance, feeding regime, production method, and seasonality were reviewed. Current trends and major research achievements in the field were discussed and commented on in detail, pointing out advantages and drawbacks typically associated with this analytical approach and arguing future improvements and changes that need to be made to recognize it as a standard and validated method for fraud mitigation and safety control in the sector of food of animal origin.

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Utility of Stable Isotope and Cytochrome Oxidase I Gene Sequencing Analyses in Inferring Origin and Authentication of Hairtail Fish and Shrimp

Cited by 18 publications

References 35 publications

Twenty‐three years of PCR‐based seafood authentication assay development: What have we learned?

Twenty‐three years of PCR‐based seafood authentication assay development: What have we learned?

Identification of Peptide Biomarkers for Discrimination of Shrimp Species through SWATH-MS-Based Proteomics and Chemometrics

Isotope Fingerprinting as a Backup for Modern Safety and Traceability Systems in the Animal-Derived Food Chain

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