Utility of SPR technology in biotherapeutic development: Qualification for intended use

Wang, Wei; Thiemann, Sandra; Chen, Qing

doi:10.1016/j.ab.2022.114804

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…Kinetic analyses were conducted at low ligand levels (150 RU). In addition to that, the TNF-α sample solution was injected at 50 μL/min to reduce mass transport limitation on the chip surface [ 35 ]. TNF-α samples were prepared with a concentration range from 3.33 to 30 nM (3-fold dilution).…”

Section: Resultsmentioning

confidence: 99%

Repurposing Fc gamma receptor I (FcγRI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -α)

Çapkın,

Kutlu,

Yüce

2023

Heliyon

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Section: Resultsmentioning

confidence: 99%

Repurposing Fc gamma receptor I (FcγRI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -α)

Çapkın,

Kutlu,

Yüce

2023

Heliyon

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“…SPR is a well-established technique that has been utilized to investigate various biophysical interactions, especially those targeting proteins. − However, using SPR for direct analysis of interactions between small molecules, such as small peptides, and proteins has commonly been a difficulty, largely due to the low signal change caused from small molecule interactions . We, therefore, utilized a competition assay to evaluate the binding of the peptide to the SARS-CoV-2-spike protein using SPR spectroscopy, with Figure providing a schematic of this competition assay.…”

Section: Resultsmentioning

confidence: 99%

Label-Free Analysis of Binding and Inhibition of SARS-Cov-19 Spike Proteins to ACE2 Receptor with ACE2-Derived Peptides by Surface Plasmon Resonance

Abouhajar

Chaudhuri

Valiulis

et al. 2022

ACS Appl. Bio Mater.

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SARS-CoV-2 has been shown to enter and infect human cells via interactions between spike protein (S glycoprotein) and angiotensin-converting enzyme 2 (ACE2). As such, it may be possible to suppress the infection of the virus via the blocking of this binding interaction through the use of specific peptides that can mimic the human ACE 2 peptidase domain (PD) α 1-helix. Herein, we report the use of competitive assays along with surface plasmon resonance (SPR) to investigate the effect of peptide sequence and length on spike protein inhibition. The characterization of these binding interactions helps us understand the mechanisms behind peptide-based viral blockage and develop SPR methodologies to quickly screen disease inhibitors. This work not only helps further our understanding of the important biological interactions involved in viral inhibition but will also aid in future studies that focus on the development of therapeutics and drug options. Two peptides of different sequence lengths, [30−42] and [22−44], based on the α 1-helix of ACE2 PD were selected for this fundamental investigation. In addition to characterizing their inhibitory behavior, we also identified the critical amino acid residues of the RBD/ACE2-derived peptides by combining experimental results and molecular docking modeling. While both investigated peptides were found to effectively block the RBD residues known to bind to ACE2 PD, our investigation showed that the shorter peptide was able to reach a maximal inhibition at lower concentrations. These inhibition results matched with molecular docking models and indicated that peptide length and composition are key in the development of an effective peptide for inhibiting biophysical interactions. The work presented here emphasizes the importance of inhibition screening and modeling, as longer peptides are not always more effective.

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“…Meanwhile, the horizontal configuration is more suitable to perform measurements in the presence of solutions, even allowing dynamic set-ups with microfluidic cells. The vertical optical device (Figure 1b) comprises a rectangular planar support (1), three planar mirrors (2), and a biconcave lens (3). The grating (4) must be positioned vertically in the special support at the center of the plane.…”

Section: The Proposed Systemmentioning

confidence: 99%

“…Surface plasmon resonance (SPR) is a valuable optical analytic tool used as a sensing technique in a wide range of applications including studies of biomolecular interactions [1,2], drug discovery [3,4], and pathogen detection [5,6]. In recent years, there has been an evergrowing interest in optical detection techniques such as absorbance [7][8][9] and SPR [10][11][12], since they guarantee high sensitivity, immunity to external disturbance, stability, and low noise [13].…”

Section: Introductionmentioning

confidence: 99%

SPECTRA: A Novel Compact System for Surface Plasmon Resonance Measurements

Pasqualotto

Cretaio

Franchin

et al. 2023

Sensors

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Surface plasmon resonance (SPR) is a common and useful measurement technique to perform fast and sensitive optical detection. SPR instrumentations usually comprise optical systems of mirrors and lenses which are quite expensive and impractical for point-of-care applications. In this work, we presented a novel and compact SPR device called SPECTRA, designed as a spectrophotometer add-on with a grating coupling configuration. The device is conceived as a marketable solution to perform quick SPR measurements in grating configuration without the requirement of complex instrumentation. The device can be customized either in a vertical structure to reach lower incident light angles, or in a horizontal configuration, which is suitable for SPR analysis using liquid solutions. The SPECTRA performance was evaluated through SPR measurements in typical applications. The vertical SPECTRA system was employed to detect different functionalization molecules on gold 720 nm-period grating devices. Meanwhile, the horizontal SPECTRA configuration was exploited to carry out fluid-dynamic measurements using a microfluidic cell with glycerol solutions at increasing concentrations to account for different refractive indexes. The experimental tests confirmed that the SPECTRA design is suitable for SPR measurements, demonstrating its capability to detect the presence of analytes and changes in surface properties both in static and dynamic set-ups.

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Utility of SPR technology in biotherapeutic development: Qualification for intended use

Cited by 8 publications

References 28 publications

Repurposing Fc gamma receptor I (FcγRI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -α)

Repurposing Fc gamma receptor I (FcγRI, CD64) for site-oriented monoclonal antibody capture: A proof-of-concept study for real-time detection of tumor necrosis factor-alpha (TNF -α)

Label-Free Analysis of Binding and Inhibition of SARS-Cov-19 Spike Proteins to ACE2 Receptor with ACE2-Derived Peptides by Surface Plasmon Resonance

SPECTRA: A Novel Compact System for Surface Plasmon Resonance Measurements

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