Utility of Serum Neuropilin-1 and Angiopoietin-2 as Markers of Hepatocellular Carcinoma

Ghafar, Muhammad Tarek Abdel; Elkhouly, Reham Abdelkader; Elnaggar, Mohammed H.; Mabrouk, Mohamed M; Darwish, Sara; Younis, Reham Lotfy; Elkholy, Rasha A

doi:10.1136/jim-2020-001744

Cited by 23 publications

(13 citation statements)

References 42 publications

(81 reference statements)

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“…And, Muhammad et al reported that serum Metadherin mRNA expression can be used for screening and early diagnosis of CRC [ 35 ]. In addition, serum neuropilin-1 and angiopoietin-2 are potential markers for hepatocellular carcinoma diagnosis [ 36 ]. Similarly, serum high-temperature-required protein A2 (HtrA2) was reported as a potential biomarker for the diagnosis of breast cancer [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer

Xun

Wang

et al. 2021

J Transl Med

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Background Colorectal cancer is one of the most common malignancy in the world. It has been reported that cancer stem cells (CSCs) serve as the primary drivers of tumorigenesis and tumor progression. There is an urgent need to explore novel molecules that regulate CSCs or their signatures. Increasing evidence has shown that miRNAs are involved in tumorigenesis and progression. Here, we aim to explore the regulatory effect and mechanism of miR-3065-3p on the stemness of colorectal cancer. Methods The expression of miR-3065-3p in colorectal cancer and the association of miR-3065-3p expression with prognosis of patients with colorectal cancer were analyzed using TCGA dataset or clinical cases. Gain or loss of function in different models, including colorectal cancer cell lines and orthotopic xenograft or liver metastatic mouse model, were used to investigate the effects of miR-3065-3p on colorectal cancer stemness and metastasis in vitro and in vivo. Cancer stemness was analyzed by detecting the ability of migration and invasion, NANOG, OCT4, and SOX2 expression, ALDH activity and sphere formation. In addition, the interaction of miR-3065-3p and cytokine receptor-like factor 1 (CRLF1) was analyzed theoretically and identified by the luciferase reporter assay. Moreover, the correlation between CRLF1 expression and miR-3065-3p was analyzed in colorectal cancer tissues. Finally, the effect of CRLF1 on the stemness and metastasis of colorectal cancer in vitro and in vivo was assessed. Results In this report, we found that miR-3065-3p was overexpressed in colorectal cancer and that its high expression was associated with poor prognosis of patients with colorectal cancer. miR-3065-3p promotes the stemness and metastasis of colorectal cancer. Furthermore, CRLF1 was the downstream target of miR-3065-3p and inhibited the stemness of colorectal cancer. In addition, CRLF1 expression was negatively correlated with miR-3065-3p in colorectal cancer tissues. And, CRLF1 mediated the effects of miR-3065-3p on promoting stemness of colorectal cancer cells. Conclusion Our data suggest that miR-3065-3p promoted the stemness and metastasis of colorectal cancer by targeting CRLF1. miR-3065-3p might serve as a promising prognostic marker as well as a therapeutic target for colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer

Xun

Wang

et al. 2021

J Transl Med

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“…Meanwhile, the mortality rate of TNBC patients within the first 5 years after diagnosis is 40% 3 . In addition, numerous readings have reported that some molecules, such as genes and protein, play vital roles in cancer treatment, bio‐labeling, and prognosis prediction 37–41 . Thus, it is of important meaning to research the molecular mechanism of the pathogenesis of TNBC in this study.…”

Section: Discussionmentioning

confidence: 90%

Circular RNA dehydrodolichyl diphosphate synthase facilitated triple‐negative breast cancer progression via miR‐362‐3p/DDX5 axis

Cui

Zhang

et al. 2022

Environmental Toxicology

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Background: Triple-negative breast cancer (TNBC) is a common hypotype of breast cancer. Circular RNAs (circRNAs) are burgeoning serve as vital controllers in numerous tumors. Nevertheless, the expression and regulatory mode of circRNAs in TNBC are still indistinct. This paper aimed to reveal the function and molecular mechanism of circular RNA dehydrodolichyl diphosphate synthase (circDHDDS) in TNBC. Methods:The contents of circDHDDS, DHDDS mRNA, and DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5) were indicated by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. The colony formation assay and 5-ethynyl-2'-deoxyuridine (EdU) assay were executed to assess cell proliferation. The flow cytometry assay was utilized to detect cell apoptosis. The transwell assay and tube formation assay were applied to measure cell migration, invasion and angiogenesis. The targeted relationships of miR-362-3p and circDHDDS or DDX5 were forecasted and detected by dual-luciferase reporter assay. The in vivo test was implemented to confirm the effect of circDHDDS. Results:The contents of circDHDDS and DDX5 were increased, and miR-362-3p level was decreased in TNBC. CircDHDDS deficiency reserved cell proliferation, migration, invasion and angiogenesis, while facilitated cell apoptosis in TNBC cells.Furthermore, miR-362-3p was validated to exert a tumor repressive effect in TNBC cells by suppressing DDX5. Moreover, DDX5 could regulate the development of TNBC. The experimental data exposed that levels of miR-362-3p presented noteworthy negative correlation with circDHDDS and DDX5, while circDHDDS and DDX5 exhibited significant positive correlation. In mechanism, circDHDDS bound to miR-362-3p to modulate DDX5 expression. In addition, circDHDDS knock-down also attenuated tumor growth. Conclusion:CircDHDDS expedited TNBC by swelling DDX5 via adapting miR-362-3p.

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“…For instance, Metadherin (AEG-1/MTDH/LYRIC) has been closely related to the poor prognosis of colorectal cancer, and can be used as a marker for the clinical diagnosis and a potential target for the treatment of colorectal cancer [ 23 , 24 ]. Serum NRP-1 and ANG-2 levels have been used as markers of hepatocellular carcinoma as their high levels are associated with advanced tumor characteristics [ 25 ]. Furthermore, our data unraveled that UCHL3 augmented the stability of AhR protein through deubiquitination, thereby promoting PD-L1 expression.…”

Section: Discussionmentioning

confidence: 99%

LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis

Yuan

et al. 2022

J Transl Med

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Background The resistance to radiotherapy remains a major obstacle that limits the efficacy of radiotherapy in non-small cell lung cancer (NSCLC). This study aims to illustrate the molecular mechanism underlying the role of LINC00665 in the radiosensitivity of NSCLC, which involves ubiquitin C-terminal hydrolase L3 (UCHL3). Methods and results The expression of UCHL3 was determined in clinical tissue samples collected from NSCLC patients and NSCLC cell lines. We found that UCHL3 overexpression occurred in both NSCLC tissues and cells, associated with poor prognosis in NSCLC patients. Mechanistically, UCHL3 stabilized aryl hydrocarbon receptor (AhR) protein through deubiquitination, thereby promoting PD-L1 expression. UCHL3 reduced the radiosensitivity of NSCLC cells by stabilizing AhR protein. Upstream microRNAs (miRNAs) and lncRNAs of UCHL3 were predicted by microarray profiling and validated by functional experiments. LINC00665 functioned as a sponge of miR-582-5p and thus up-regulated the expression of the miR-582-5p target UCHL3. Gain- and loss- of function assays were performed to assess the effects of LINC00665, UCHL3 and miR-582-5p on the in vitro cell malignant behaviors and immune escape as well as on the in vivo tumor growth. Silencing LINC00665 or overexpressing miR-582-5p enhanced the sensitivity of NSCLC cells to radiotherapy. LINC00665 augmented the immune escape of NSCLC cells in vitro and in vivo through stabilizing AhR protein via the miR-582-5p/UCHL3 axis. Conclusions Overall, LINC00665 reduced the radiosensitivity of NSCLC cells via stabilization of AhR through the miR-582-5p/UCHL3 axis.

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Utility of Serum Neuropilin-1 and Angiopoietin-2 as Markers of Hepatocellular Carcinoma

Cited by 23 publications

References 42 publications

miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer

miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer

Circular RNA dehydrodolichyl diphosphate synthase facilitated triple‐negative breast cancer progression via miR‐362‐3p/DDX5 axis

LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis

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