Utility of real-time PCR for the diagnosis of infantile visceral leishmaniasis in Algeria

Mihoubi, Ilhem; Monbrison, Frédérique de; Frahtia, K.; Picot, Stéphane; Gassem, Nadia

doi:10.1684/mst.2012.0011

Cited by 4 publications

(5 citation statements)

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“…Since the 1980s, CL incidence has spread in North African countries, mainly in Algeria, where cases range from 10,000 to 40,000 per year [1,10], making this country among the most affected by CL. This vectorborne disease is reported in several regions from the north to south and east to west of Algeria [12,20,37,47,[67][68][69].…”

Section: Discussionmentioning

confidence: 99%

Human Cutaneous Leishmaniasis in North Africa and Its Threats to Public Health: A Statistical Study Focused on Djelfa (Algeria)

Messaoudene,

Boukraa,

Boubidi

et al. 2023

Microorganisms

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Cutaneous leishmaniasis, the most common form of leishmaniasis, causes long-term skin lesions on exposed areas of the skin. It is caused by a protozoan parasite belonging to the genus Leishmania and is transmitted via infected phlebotomine sand flies. In North Africa, particularly Algeria, the disease represents a major public health problem. This retrospective study, which focuses on the agropastoral region of Djelfa (central Algeria) during a period of 16 years, from 2006 to 2021, is part of the surveillance of cutaneous leishmaniasis to identify the key factors favouring its probable spread. The analyzed data reveal that this disease is more prevalent in male patients (53.60%) and is highly widespread in this vast area of 66,415 km2 with a total of 3864 CL cases, reaching a peak of 1407 cases in 2006. Statistically, the Pearson correlation validated by the p-value shows, in an original and sometimes unexpected way, that certain factors, such as temperature linked to climate change, are playing a significant role in the probable spread of the disease in Djelfa and its surrounding regions. The concentration of the population in some specific rural areas with limited or nonexistent access to public health services is another potential factor in disease transmission. The results were highlighted by a significant correlation coefficient (r=0.66) with a p-value less than 0.01. While there is currently no vaccine or prophylactic drug available, our research represents a preliminary approach that addresses various epidemiological aspects of the disease. This paves the way for a proactive preventive strategy involving the control of vector-borne diseases.

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Section: Discussionmentioning

confidence: 99%

Human Cutaneous Leishmaniasis in North Africa and Its Threats to Public Health: A Statistical Study Focused on Djelfa (Algeria)

Messaoudene,

Boukraa,

Boubidi

et al. 2023

Microorganisms

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“…In the second step, only the positive samples revealed by the conventional PCR were considered for qPCR quantification of Leishmania parasites load, the reactions were performed in Mic Real-Time PCR Cycler Systems using SuperScript III Platinium SYBR Green One-Step qRT-PCR Kit detection method (Invitrogen, Life Technology) using minicircle kinetoplast DNA as the molecular target ( Myskova et al, 2008 ). As reported previously, the qPCR was considered specific when the melting temperature was 84 °C for the positive controls ( Mihoubi et al, 2006 ).…”

Section: Methodsmentioning

confidence: 99%

Host competence of Algerian Gerbillus amoenus for Leishmania major

Benallal¹,

Mezai²,

Mefissel³

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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“…Furthermore, Mihoubi et al. [10] stated that leishmaniasis represents 35% of notifiable diseases in Algeria, leading to its first rank among parasitic diseases in Algeria. Dogs are one of the main reservoirs of the parasite, and Phlebotomus insects are the vectors.…”

Section: Epidemiology Of Major Zoonotic Diseases In Algeriamentioning

confidence: 99%

“…Human visceral leishmaniasis (VL) caused by Leishmania infantum (MON-1 and MON-24), conveyed by Phlebotomus perniciosus with dogs as the reservoir, is the most severe form of leishmaniasis [10]. It has been recorded in the north of Algeria in a humid bioclimatic area, but it is spreading throughout the country [12].…”

Section: Epidemiology Of Major Zoonotic Diseases In Algeriamentioning

confidence: 99%

“…It has been recorded in the north of Algeria in a humid bioclimatic area, but it is spreading throughout the country [12]. Mortality is observed in children from 0 to 4 years old, in whom the incidence is 2.72 cases per 100 000 inhabitants [10]. Moreover, data obtained from the Algerian Institute of Public Health [6] from 2010 to 2017 showed an average of 47 cases (range, 30–84 cases) of VL per year.…”

Section: Epidemiology Of Major Zoonotic Diseases In Algeriamentioning

confidence: 99%

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Epidemiology of dog-mediated zoonotic diseases in Algeria: a One Health control approach

Kardjadj

Ben-Mahdi

2019

New Microbes and New Infections

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Globally, human–dog interactions cause significant social, economic and human health costs. Public health problems linked with dogs include mainly zoonotic diseases. Recently the concept of a global and integrative approach to improve the health and well-being of people, animals and the environment has been strongly endorsed. This concept, often referred to as One Health, also reflects the collaboration in the field of surveillance and monitoring. Because humans and animals often suffer from the same pathogens and share the same environment, a cross-sector approach integrating human and animal disease surveillance information is required. The aims of the present study were to describe the incidence of dog-mediated zoonotic diseases in Algeria between 2010 and 2017, and to propose a One Health approach to control these diseases in Algeria. Rabies, leishmaniasis and echinococcosis are the major zoonoses in Algeria, with a reported average number of deaths per year, respectively, of 18, 7947 and 387. These zoonoses occur with the uncontrolled proliferation of household waste deposits, particularly in and around urban area which maintain the presence of stray dogs. The persistence of these diseases indicates the need for greater partnership and collaboration among multiple sectors, including medical doctors, veterinarians, ecologists, environmentalists and law-enforcement agents. Such partnerships permit the sharing of information, facilities and resources under a One Health approach; permit rapid communication among disciplines as well as interdisciplinary training/education opportunities and raising awareness among human population; and allow a combined effort towards disease surveillance/control, which will consequently improve the efficiency of the control programmes.

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Utility of real-time PCR for the diagnosis of infantile visceral leishmaniasis in Algeria

Cited by 4 publications

References 0 publications

Human Cutaneous Leishmaniasis in North Africa and Its Threats to Public Health: A Statistical Study Focused on Djelfa (Algeria)

Human Cutaneous Leishmaniasis in North Africa and Its Threats to Public Health: A Statistical Study Focused on Djelfa (Algeria)

Host competence of Algerian Gerbillus amoenus for Leishmania major

Epidemiology of dog-mediated zoonotic diseases in Algeria: a One Health control approach

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