Utility of quantitative analysis of urine lipoarabinomannan in the diagnosis of tuberculosis

Agha, Mohammed A.; El-Helbawy, Rana; El-Helbawy, Nessrin G.; El-Sheak, Neveen M.

doi:10.1016/j.ejcdt.2013.06.007

Cited by 9 publications

(5 citation statements)

References 15 publications

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“…Our results are controversary with Agha et al, [7] who reported that Urine LAM test had sensitivity, specificity, PPV, NPV, and an accuracy of 81.2%, 95.7%, 97.2%, 73.8%, and 86.4% respectively. In another study martinson et al, [21] reported that sensitivity and specificity of a positive LAM for culture-confirmed tuberculosis were 65% and 86% respectively and with Youssef et al, [22] reported that quantitative urine LAM had a sensitivity of 85.5%, specificity 90%, accuracy 86.1%, positive predictive value 98.1%, and negative predictive value 50%.…”

Section: Discussioncontrasting

confidence: 99%

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Different Methods in Diagnosis of Pulmonary and Extrapulmonary Tuberculosis

Rashed¹,

El-Rahman²,

Ahmed

et al. 2018

The Medical Journal of Cairo University

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Background: Tuberculosis (TB) has been a major health problem in developing countries.Rapid diagnosis of Mycobacterium tuberculosis infection plays a critical role in controlling the spread of tuberculosis. Conventional methods may take up to several weeks or longer to produce results. This study aimed to compare different methods like culture on Bio F-M media, automated BacT/ ALERT MP bottles, and urine lipoarabinomnnan by ELISA. Subjects and Methods:This study was done on 100 patients suspected to have tuberculosis, cases divided into two groups: (Group A): 68 clinically suspected pulmonary TB cases. (Group B): 32 clinically suspected extrapulmonary TB cases. All samples from Group A and B were subjected to direct staining by Zeihl-Neelsen stain, culture of the samples on Lowenstein Jensen media, Bio FM media and automated BacT/ALERT MP bottles and detection of urine lipoarabinomannan by ELISA.Results: In suspected pulmonary tuberculosis cases the sensitivity and specificity of Bio FM were 87.2%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 74.4%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 20.5%, 96.9% respectively and in suspected extrapulmonary tuberculosis cases were the sensitivity and specificity of Bio FM were 87.5%, 100% respectively, sensitivity and specificity of BacT/ALERT MP were 50%, 100% respectively, sensitivity and specificity of urine lipoarabinomannan were 12.5%, 100% respectively. Conclusion:The sensitivity of both solid (Lowenstein Jensen) and liquid (Bio FM) media were the same and better than BacT/ALERT MP. Bio FM media and BacT/ALERT MP show shorter time than Lowenstein Jensen for detection of mycobacterial growth. Detection of urine lipoarabinomannan by ELISA is insensitive for the diagnosis of TB.

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Section: Discussioncontrasting

confidence: 99%

“…Lipoarabinomannan (LAM), a 17.5 kD glycolipid component of the outer cell wall of mycobacteria is an attractive diagnostic target [6] . LAM is released when Mycobacterium tuberculosis is lysed by the host immune system filtered by the kidneys and can be detected in the urine as a potential same day diagnostic test for tuberculosis [7] .…”

Section: Introductionmentioning

confidence: 99%

Different Methods in Diagnosis of Pulmonary and Extrapulmonary Tuberculosis

Rashed¹,

El-Rahman²,

Ahmed

et al. 2018

The Medical Journal of Cairo University

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“…7,12,13 LAM is a widely known and structurally important 17.5 kDa lipopolysaccharide found in mycobacterial cell walls. 12,[14][15][16] When M. tuberculosis is lysed by the host immune system, it is filtered by the kidneys and LAM can then be detected in the urine. 15 Since no human glycosidases are known for the degradation of LAM, an antigen with glycosidic linkages, it is a key potential biomarker in urine.…”

mentioning

confidence: 99%

“…12,[14][15][16] When M. tuberculosis is lysed by the host immune system, it is filtered by the kidneys and LAM can then be detected in the urine. 15 Since no human glycosidases are known for the degradation of LAM, an antigen with glycosidic linkages, it is a key potential biomarker in urine. 13 Several studies have confirmed the presence of LAM antigens in the urine of patients with active TB 12,17 and have found that the amount of LAM in urine is correlated with the bacterial burden metabolic activity and rate of degradation of the bacteria, and hence allows for a semiquantitative assessment of the infection.…”

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confidence: 99%

Label-Free and Real-Time Detection of Tuberculosis in Human Urine Samples Using a Nanophotonic Point-of-Care Platform

et al. 2018

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Tuberculosis (TB) is the leading global cause of death from a single infectious agent. Registered incidence rates are low, especially in low-resource countries with weak health systems, due to the disadvantages of current diagnostic techniques. A major effort is directed to develop a point-of-care (POC) platform to reduce TB deaths with a prompt and reliable low-cost technique. In the frame of the European POCKET Project, a novel POC platform for the direct and non-invasive detection of TB in human urine was developed. The photonic sensor is integrated in a disposable cartridge and is based on a highly sensitive Mach-Zehnder Interferometer (MZI) transducer combined with an on-chip spectral filter. The required elements for the read-out are integrated in an instrument prototype, which allows real-time monitoring and data processing. In this work, the novel POC platform has been employed for the direct detection of lipoarabinomannan (LAM), a lipopolysaccharide found in the mycobacterium cell wall. After the optimization of several parameters, a limit of detection of 475 pg/mL (27.14 pM) was achieved using a direct immunoassay in undiluted human urine in less than 15 minutes. A final validation of the technique was performed using twenty clinical samples from TB patients and healthy donors, allowing the detection of TB in people regardless of HIV coinfection. The results show excellent correlation to those obtained with standard techniques. These promising results demonstrate the high sensitivity, specificity and applicability of our novel POC platform, which could be used during routine checkups in developing countries.

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“…Then we went over the text, and 95 studies were eliminated for various reasons. Finally, 67 articles with a total of 164 reports and 23727 samples were included 6 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , ...…”

Section: Resultsmentioning

confidence: 99%