Utility of PAS and β-catenin staining in histological categorisation and prediction of prognosis of hepatoblastomas

Bera, Goutam; Das, Ram; Roy, Paromita; Ghosh, Ranajoy; Islam, Nelofar; Mishra, Prafulla Kumar; Chatterjee, Uttara

doi:10.1007/s00383-017-4115-2

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…Both absolute ␤-catenin level and nuclear localization are of prognostic significance in some HB studies, although the degrees to which these are mutually and mutationally dependent have not been determined (3,58,74). Missense and in-del mutations can profoundly affect mRNA stability by altering folding, splicing, recognition by micro-RNAs, and translational efficiency (75)(76)(77).…”

Section: ␤-Catenin Mutations and Hepatoblastoma Phenotypesmentioning

confidence: 99%

β-Catenin mutations as determinants of hepatoblastoma phenotypes in mice

Zhang

Meyfeldt

Wang

et al. 2019

Journal of Biological Chemistry

185

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Hepatoblastoma (HB) is the most common pediatric liver cancer. Although long-term survival of HB is generally favorable, it depends on clinical stage, tumor histology, and a variety of biochemical and molecular features. HB appears almost exclusively before the age of 3 years, is represented by seven histological subtypes, and is usually associated with highly heterogeneous somatic mutations in the catenin β1 (CTNNB1) gene, which encodes β-catenin, a Wnt ligand–responsive transcriptional co-factor. Numerous recurring β-catenin mutations, not previously documented in HB, have also been identified in various other pediatric and adult cancer types. Little is known about the underlying factors that determine the above HB features and behaviors or whether non-HB–associated β-catenin mutations are tumorigenic when expressed in hepatocytes. Here, we investigated the oncogenic properties of 14 different HB– and non-HB–associated β-catenin mutants encoded by Sleeping Beauty vectors following their delivery into the mouse liver by hydrodynamic tail-vein injection. We show that all β-catenin mutations, as well as WT β-catenin, are tumorigenic when co-expressed with a mutant form of yes-associated protein (YAP). However, tumor growth rates, histologies, nuclear-to-cytoplasmic partitioning, and metabolic and transcriptional landscapes were strongly influenced by the identities of the β-catenin mutations. These findings provide a context for understanding at the molecular level the notable biological diversity of HB.

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Section: ␤-Catenin Mutations and Hepatoblastoma Phenotypesmentioning

confidence: 99%

β-Catenin mutations as determinants of hepatoblastoma phenotypes in mice

Zhang

Meyfeldt

Wang

et al. 2019

Journal of Biological Chemistry

185

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“…Their study concludes that both these markers were beneficial in the pretreatment group and post-treatment specimen. 8,17 tumours were positive for Beta-catenin in both epithelial and mesenchymal components in the pretreatment group. The epithelial component of all the untreated cases showed intense cytoplasmic Glutamine synthetase staining, and malignant mesenchymal elements in all the cases were negative.…”

Section: Discussionmentioning

confidence: 89%

“…Our study was in accordance with many other studies done on staining patterns in hepatoblastoma worldwide. 17,18…”

Section: Discussionmentioning

confidence: 99%

Pattern of Different Immunohistochemical Stains in Various Types of Hepatoblastoma

Maqbool

Mushtaq²,

Hussain³

et al. 2022

PAFMJ

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Objective: To assess the utility of four immunohistochemical stains in the categorization of hepatoblastoma. Study Design: Retrospective longitudinal study. Place and Duration of Study: Histopathology Department, Shaukat Khanum Memorial Cancer Hospital, Peshawar Pakistan, from 2016 to 2018. Methodology: We retrospectively reviewed 50 cases of hepatoblastoma from 2016-2018, from our hospital database and studied the pattern of four immunohistochemical stains (Beta-catenin, Glutamine synthetase, Heppar 1 and Cyclin D1) in various subtypes. Results: Ten out of fifty cases were mixed epithelial and mesenchymal hepatoblastomas, and forty were pure epithelial. In the pure epithelial category, the fetal subtype showed Heppar 1 and Cyclin D1 positivity in twenty-eight cases, while Glutamine synthetase and Beta-catenin were positive in all the thirty cases. One case of embryonal subtype displayed negativity for Heppar 1; the rest of the immunohistochemical stains were positive. Ten tumours exhibiting mixed epithelial and mesenchymal morphology showed positivity for all the four immunohistochemical stains in the epithelial component. However, Heppar 1 and cyclin D1 were negative in the mesenchymal component in all the ten cases. Five cases (50%) showed Glutamine synthetase and Beta-catenin positivity in the mesenchymal component. Conclusion: All the four immunohistochemical stains, especially Beta-catenin and Glutamine synthetase, were efficient diagnostic tool, especially for tumours with complex or vague morphological features.

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“…CD34 shows diffuse capillarization in HB, which is not seen in benign lesions and normal hepatocytes, indicating that sinusoids are lined by endothelial cells in the tumor. Beta-catenin gives nuclear and cytoplasmic positivity in both HB and normal hepatocytes, which has diagnostic and prognostic significance in HB [11]. The SCUD subtype shows the variable expression for PanCK, CK8, CK-18, and vimentin.…”

Section: Discussionmentioning

confidence: 99%

Common and Rare Histological Variants of Hepatoblastoma in Children: A Pathological Diagnosis and Review of the Literature

Bharti

Sinha

Yadav

2021

Gastrointest Tumors

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Hepatoblastoma (HB) is a rare tumor, but it is the most common primary liver malignancy in children and comprised of approximately 1% of all pediatric malignancies. Mostly, this tumor is sporadic in nature but can show a syndrome association. Upregulation in Wnt/β-catenin pathway can be there in 70–80% cases of HB. Most often present as abdominal mass and has a raised alpha-fetoprotein levels. Distant metastasis usually occurs in the lungs. HB is classified into 2 broad categories: epithelial and mesenchymal type. The majority of HB are epithelial type. The HB must be distinguished from focal nodular hyperplasia, hepatocellular adenoma, and hepatocellular carcinoma, while small-cell undifferentiated HB from the malignant rhabdoid tumor. The histomorphology and immunohistochemistry are essential for the diagnosis of different HB. The neoadjuvant chemotherapy followed by surgery is the mainstay of the treatment. The 2 well-established prognostic factors of HB are stage and histological type. Herein, we report a case series of common and rare histological variants of HB.

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Utility of PAS and β-catenin staining in histological categorisation and prediction of prognosis of hepatoblastomas

Abstract: PAS is a simple stain and can be utilised in histological categorisation of HB and also predicting its outcome. Nuclear β-catenin staining which is significantly common in embryonal elements in HB predicts shorter survival.

Cited by 5 publications

References 22 publications

β-Catenin mutations as determinants of hepatoblastoma phenotypes in mice

β-Catenin mutations as determinants of hepatoblastoma phenotypes in mice

Pattern of Different Immunohistochemical Stains in Various Types of Hepatoblastoma

Common and Rare Histological Variants of Hepatoblastoma in Children: A Pathological Diagnosis and Review of the Literature

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