Utility of NBD-Cl as an electrophilic reagent for the determination of the two antihypertensive drugs hydrochlorothiazide and minoxidil in dosage forms and human urine samples

Utility of green chemistry for sustainable fluorescence derivatization approach for spectrofluorimetric quantification of Darolutamide as antineoplastic drug in pharmaceutical formulation and spiked human plasma

“…The fluorescence derivatization approach involves converting naturally nonfluorescent substances into fluorescent derivatives using a simple reaction with derivatizing reagents such as NBD-Cl, fluorescamine, ninhydrin, and O-phthalaldehyde [16][17][18]. The nucleophilic substitution reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl) is utilized in fluorimetric derivatization processes using amine groups (primary and secondary) to yield highly fluorogenic products in alkaline medium [19,20]. In this work, we developed a spectrofluorimetric approach for estimating DAR in pharmaceutical preparations and plasma samples that are very sensitive, simple, inexpensive, and ecologically favourable.…”