Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis

Raveendran, Reena; Wattal, Chand

doi:10.1016/j.bjid.2016.01.006

Cited by 16 publications

(14 citation statements)

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“…12 A study by Wattal et al, conducted to assess multiplex PCR using IS6110 and MPB64 for EPTB found that IS6110 had a better sensitivity (90.3%) compared to MPB64 (64.5%). 25 Negi et al and Singh et al reported better yield when IS6110 was used for tuberculous lymphadenitis. 24,26 Recent studies, including those mentioned above, report a high accuracy of multiplex PCR for different forms of EPTB like lymph node, skin, osteoarticular, and meningeal tuberculosis.…”

Section: Discussionmentioning

confidence: 98%

“…In a study to evaluate multiplex PCR using Protein b, MPB64, and IS6110 primers for diagnosis of tuberculous meningitis, sensitivity of microscopy, culture, and multiplex PCR was 1.81, 16.73, and 86.63% and specificity was 100, 100, and 100%, respectively . A study by Wattal et al, conducted to assess multiplex PCR using IS6110 and MPB64 for EPTB found that IS6110 had a better sensitivity (90.3%) compared to MPB64 (64.5%) . Negi et al and Singh et al reported better yield when IS6110 was used for tuberculous lymphadenitis …”

Section: Discussionmentioning

confidence: 99%

“…Recent studies, including those mentioned above, report a high accuracy of multiplex PCR for different forms of EPTB like lymph node, skin, osteoarticular, and meningeal tuberculosis . However, after a review of PubMed, we could trace only two studies that evaluated the role of multiplex PCR in abdominal tuberculosis, and more specifically, GITB .…”

Section: Discussionmentioning

confidence: 99%

“…24,26 Recent studies, including those mentioned above, report a high accuracy of multiplex PCR for different forms of EPTB like lymph node, skin, osteoarticular, and meningeal tuberculosis. 12,13,25,26 However, after a review of PubMed, we could trace only two studies that evaluated the role of multiplex PCR in abdominal tuberculosis, and more specifically, GITB. 14 15 The results of our study are consistent with the above two studies and highlight the highly sensitive and promising role of multiplex PCR in rapid and accurate diagnosis of GITB.…”

Section: Discussionmentioning

confidence: 99%

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Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis

et al. 2018

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Background and Aim To evaluate the role of multiplex polymerase chain reaction (PCR) for diagnosis of gastrointestinal tuberculosis (GITB). Methods This was a prospective observational study conducted from July 2015 to November 2016 at a tertiary care teaching institution in north India. Fifty individuals with clinically suspected GITB and older than 18 years of age were recruited. Patients underwent radiological investigations, esophagogastroduodenoscopy, or colonoscopy as clinically indicated. Multiple biopsies for tissue diagnosis and PCR were taken. All specimens were subjected to Ziehl Neelsen staining, histopathology, and multiplex PCR using specific primers for genes IS6110, MPB64, and Protein b. The performance of the assay was assessed using a composite reference standard for diagnosis of tuberculosis. It comprised a combination of clinical characteristics and microbiological methods, including smear, Bactenecin (BACTEC) culture, histopathology, and response to antitubercular therapy. Results A final diagnosis of tuberculosis was made in 32 cases (Duodenal‐4, Ileo‐colonic‐28). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of histopathology for the diagnosis of tuberculosis was 28.12, 100, 100, and 43.9%, respectively. The sensitivity, specificity, PPV and NPV of BACTEC Mycobacteria Growth Indicator Tube (MGIT) culture for the diagnosis of tuberculosis was 9.3, 100, 100, and 38.29%, respectively. The sensitivity, specificity, PPV, and NPV of multiplex PCR for the diagnosis of tuberculosis was 87.5, 100, 100, and 86.2%, respectively. Conclusion Multiplex PCR using specific primers for genes IS6110, MPB64, and Protein b had a higher sensitivity compared to conventional techniques for the diagnosis of GITB.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis

et al. 2018

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“…PCR methods have been promising in TB diagnosis [2,4]. Meena et al have shown that multiplex PCR works for spinal TB too, using three target genes, namely IS6110, protein b, and MPB64.…”

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confidence: 99%

Towards a timely diagnosis of spinal tuberculosis

Demetriades

2017

Acta Neurochir

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Tuberculosis (TB) remains a major global public health concern, even though it is a treatable and curable disease. Statistics from the World Health Organization (WHO) show that TB occurs all over the globe; there were 9.6 million new cases in 2014, a prevalence of 133/100,000, and 1.5 million deaths. The largest incidence was in the WHO South-East Asia and WHO Western Pacific regions, where 58% of all new global cases were encountered. Furthermore, the incidence was >10 times greater in low-income countries, and the mortality >20 times greater than in high-income countries. This reflects, in part, the large inequality of access to health services both for diagnosis and for treatment [5].In Europe, TB continues to present a major public health challenge. It is estimated that approximately 340,000 new cases and 33,000 deaths were reported in 2014, mostly from eastern and central European countries. A false sense of security might be conveyed when considering data showing that in the last 10 years there has been a decrease of 5.2% per annum in new cases of TB. In fact, notification rates have remained ∼8 times higher in high-TB-priority countries than in the rest of the WHO Europe region [1].With the ongoing globalization, migration, and mixing of populations, TB is now more prevalent in certain pockets of Europe than it has been for a very long time. The WHO Regional Office for Europe confirms it is most frequently seen amongst young adults in the eastern part of the region, and among migrants and the elderly native population in Western European countries. TB is intrinsically connected to social determinants of health (e.g., poverty, migration, social mar-Another challenge is that treatment results in Europe are not ideal; 76% treatment success in new/relapsed cases in 2013, and approximately 50% treatment success in multidrug-resistant TB (MDR-TB) in 2012. Additionally, a persisting further challenge has been the timely diagnosis.Indeed, microbiological confirmation of TB infection of the spine can be typically very slow. A relatively small but prospective study of nine patients employed multiplex PCR in obtaining the diagnosis [3]. Seven out of nine patients were positive for TB on eventual diagnosis, with multiplex PCR having one false-negative case; the authors speculate due to bacterial lysis during processing or anti-TB treatment having commenced prior to the test. Sensitivity was 85.7% and specificity was 100%. By comparison, AFB testing was only positive in 66.6% and culture was only positive in 22.2% of cases.Meta-analysis data has shown an overall pick-up rate of smear and culture as 37 and 66.2%, respectively. As such, smear has a low pick-up rate, whereas culture has a better pick-up rate, but its main disadvantage is the length of time required.Differences in time-to-diagnosis within this study were significant: <24 h for multiplex PCR vs. 45 days for culture [3].PCR methods have been promising in TB diagnosis [2,4]. Meena et al. have shown that multiplex PCR works for spinal TB too, using three targ...

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Pathology of Tuberculosis

Rammeh

2022

Imaging of Tuberculosis

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Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis

Abstract: PCR can play an important role in rapid and accurate diagnosis of extrapulmonary tuberculosis. IS6110 alone is an effective target in our part of the country.

Cited by 16 publications

References 19 publications

Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis

Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis

Towards a timely diagnosis of spinal tuberculosis

Pathology of Tuberculosis

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