Utility of intraoperative squash cytology in diagnosis of paediatric central nervous system lesions

Abstract

Background Role of squash cytology in intraoperative diagnosis of central nervous system lesions has been well established. Intraoperative diagnosis is especially important in paediatric CNS lesions as decision regarding gross total resection or near total resection or subtotal resection is crucial and radiotherapy and chemotherapy are best avoided in this age group. The aim of this study was to evaluate the utility of squash cytology of CNS lesions in paediatric age group and to assess its diagnostic accuracy, sensitivity, specificity, positive predictive value and negative predictive value. Materials and methods A prospective study was conducted on 42 paediatric patients with clinico‐radiologically diagnosed CNS lesions. Intraoperative squash smears were stained with haematoxylin and eosin (H&E) stain. Diagnosis made subsequently on paraffin sections was taken as gold standard. Results The overall diagnostic accuracy of intraoperative squash cytology of CNS lesions in paediatric age group was 73.80%. The sensitivity, specificity, positive predictive value and negative predictive value of this modality were 92.31%, 87.50%, 96.00% and 77.78%, respectively. Conclusions The spectrum of CNS lesions in paediatric age group is different from that in adults. Though the sensitivity and specificity of squash cytology in paediatric tumours are less than that of adults, it is an important tool for intraoperative diagnosis that guides regarding the extent of resection.

Unveiling the potential application of intraoperative brain smear for brain tumor diagnosis in low-middle-income countries: A comprehensive systematic review

“…A total of 59 articles were included in the final analysis. [ 2 , 4 - 6 , 10 - 21 , 23 - 29 , 31 - 33 , 35 - 37 , 39 , 42 , 44 - 47 , 49 , 50 , 52 , 54 - 67 , 70 - 75 ] The majority of studies included in this review were conducted in LMICs, with single-center retrospective or prospective designs. Many of these studies were conducted in India.…”

“…A total of 59 articles were included in the final analysis. [2,[4][5][6][10][11][12][13][14][15][16][17][18][19][20][21][23][24][25][26][27][28][29][31][32][33][35][36][37]39,42,[44][45][46][47]49,50,52,[54][55][56][57][58][59][60][61][62][63][64][65][66][67][70]…”

Nöropatolojide İntraoperatif Konsültasyonda Tanısal Yaklaşımın Kurumsal Deneyimi

“…İntraoperatif tanı (İOT) ile immunhistokimyasal (İHK) ve/veya moleküler incelemelerle konulan nihai tanı icin doku yeterliliğinin belirlenmesi, cerrahi sınırların tespiti, neoplastik olgularda tümör tipinin belirlenmesiyle cerrahinin yönlendirilmesi amacļanır [4][5][6] . Pediatrik yaş grubunda doğru IȮT, total rezeksiyonu mümkün vakalarda kemoterapi ve radyoterapinin etkilerinden kacinmak açısından çok önemlidir 7,8 . Enfeksiyon veya lenfomalarda İOT ile mikrobiyolojik ya da flow sitometrik incelemeler icin doku yeterliliği değerlendirilebilir 9 .…”