Utility of egg yolk medium for cultivation of Mycoplasma pneumoniae

Sasaki, Tsuguo; Shintani, Miharu; Kihara, K

doi:10.1128/jcm.18.5.1167-1173.1983

Cited by 5 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inhibitory Effect of HS on the Growth of M. pneumoniae and Some Other Mycoplasma l Species In our previous study (8), we found that the CFU of M. pneumoniae in broth medium containing HS decreased after incubation for one day. Therefore the effect of nine lots of HS on the number of CFU of M. pneumoniae strain FH after incubation for 24 hr in HS medium was investigated ( Table 1).…”

Section: Resultsmentioning

confidence: 82%

“…EY. EY was prepared as previously described (8). Briefly, yolks from fresh unfertilized chicken eggs were suspended in sterile phosphate-buffered saline without Ca2+ and Mg2+, pH 7.2, at a concentration of 20% (w/v).…”

Section: Methodsmentioning

confidence: 99%

“…After the publication of our previous paper (8) in which we mentioned the sufficient transparency of EY agar medium, we found that EY agar medium made with PPLO agar (Difco Laboratories, Detroit, Mich.) or with PPLO broth plus some agar preparations was very turbid. However, EY agar medium made with Special Agar A (Wako Pure Chemical Industries, Ltd., Osaka, prepared from Ahnfeltia plicata), Special Agar B (prepared from Gracilaria confervoides), which was employed in our previous work (8), or agar agar powder (Hachiko Agar Co., Ltd., Tokyo) was always transparent. This turbidity might be due to the formation of insoluble molecular complexes between lipoproteins in EY and some high molecular components of agar preparations (1,3,9).…”

Section: Effect Of Hs On the Metabolism-inhibition Testmentioning

confidence: 99%

“…EY medium showed good growthsupporting activity for laboratory-adapted strains and clinical isolates of M. pneumoniae. This might be due to the absence of a growth inhibitor and to the large amount of lipoproteins rich in cholesterol and phospholipids in EY (8).…”

Section: Effect Of Hs On the Metabolism-inhibition Testmentioning

confidence: 99%

“…In our previous study (8), we showed that EY, the supernatant fluid of chicken egg yolk suspension in phosphate-buffered saline, was useful as a serum substitute in mycoplasmal culture media.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Comparison of the Efficacy of Egg Yolk Extract and Horse Serum for Growth of Mycoplasma pneumoniae

Sasaki¹,

Shintani²,

Kihara³

1985

Microbiology and Immunology

View full text Add to dashboard Cite

The usefulness of chicken egg yolk extract as a substitute for horse serum in culture media for Mycoplasma pneumoniae was investigated.As a growth-supporting factor in the growth medium for M. pneumoniae and some other mycoplasmal species, the primary isolation medium for M. pneumoniae, and the metabolism-inhibition test medium for diagnosis of M. pneumoniae infection, egg yolk extract may be an excellent substitute for horse serum.The particular superiority of egg yolk extract to horse serum is that egg yolk extract, unlike horse serum, did not show any inhibitory effect on the growth of M. pneumoniae.Conventional culture media for mycoplasmas contain 20 % (v/v) unheated horse serum (HS) (2, 6). As the growth of Mycoplasma pneumoniae is often markedly affected by certain HS preparations, it is important to use HS preparations of suitable quality in the medium for diagnosis of M. pneumoniae infection. Recently SP-4 medium, which contains fetal bovine serum heated at 56 C for 1 hr (11), and modified New York City medium, which contains agamma HS (4, 5), have been introduced as excellent primary isolation media for M. pneumoniae.In our previous study (8), we showed that EY, the supernatant fluid of chicken egg yolk suspension in phosphate-buffered saline, was useful as a serum substitute in mycoplasmal culture media.In the present study, we showed the inhibitory effect of HS on the growth of M. pneumoniae, and compared EY broth medium, which consists of 90 parts of PPLO broth and 10 parts of EY, with Chanock medium (2), which contains20 parts of unheated HS, for their ability to support the growth of laboratory-adapted strains and clinical isolates of M. pneumoniae. The latter is a medium used very frequently as one of the conventional growth media for mycoplasmas. MATERIALS AND METHODSMycoplasmas. Of the ,1.5 mycoplasmal strains listed in Table 2,. M. arginini and M. hominis were obtained from Prof. M. Nakamura of Kurume University School of Medicine. The other strains were obtained from Prof. M. Ogata of Azabu University of Veterinary Medicine and have been maintained in our laboratory for 49 9

show abstract

Section: Resultsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Effect Of Hs On the Metabolism-inhibition Testmentioning

confidence: 99%

Section: Effect Of Hs On the Metabolism-inhibition Testmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Comparison of the Efficacy of Egg Yolk Extract and Horse Serum for Growth of Mycoplasma pneumoniae

Sasaki¹,

Shintani²,

Kihara³

1985

Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

Utility of Egg Yolk Medium for Cultivation of Some Mycoplasma Species

Sasaki

Kihara

1987

Microbiology and Immunology

View full text Add to dashboard Cite

Interaction of albumin and phospholipid:cholesterol liposomes in growth of Mycoplasma spp

Cluss¹,

Somerson²

1986

Appl Environ Microbiol

View full text Add to dashboard Cite

Mycoplasma spp., sterol and fatty acid auxotrophs, are conventionally grown in complex media containing high concentrations of serum. Serum supplies the required lipids, but its presence complicates studies on the metabolism and antigenicity of mycoplasmas as well as the membrane dynamics of these organisms. In the present work, fetal bovine serum was replaced with dilipidated albumin and liposomes containing high concentrations of cholesterol. The liposomes were produced from phosphatidylcholine which contained other lipid species, including phosphatidylethanolamine, phosphatidylglycerol, and cholesterol. Other liposomes containing cholesterol and one phospholipid yielded significantly less growth of Mycoplasma gallisepticum, indicating that several phospholipids are required to achieve growth levels comparable to those obtained with complex medium. The sources and concentrations of cholesterol, albumin, phosphatidylcholine, and other phospholipids and the interactions among them were important affectors of mycoplasmal growth. Optimal lipid and albumin conditions established for M. gaUlisepticum were then used to propagate five diverse Mycoplasma spp. to growth levels which equalled or surpassed those obtained with medium containing 17% fetal bovine serum.

show abstract

Utility of egg yolk medium for cultivation of Mycoplasma pneumoniae

Cited by 5 publications

References 23 publications

Comparison of the Efficacy of Egg Yolk Extract and Horse Serum for Growth of Mycoplasma pneumoniae

Comparison of the Efficacy of Egg Yolk Extract and Horse Serum for Growth of Mycoplasma pneumoniae

Utility of Egg Yolk Medium for Cultivation of Some Mycoplasma Species

Interaction of albumin and phospholipid:cholesterol liposomes in growth of Mycoplasma spp

Contact Info

Product

Resources

About